Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00710

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmdtK and ydhQ are separated by 40 nucleotidesydhQ and Val_trna are separated by 307 nucleotidesVal_trna and Val_trna are separated by 4 nucleotidesVal_trna and ydhR are separated by 107 nucleotides NIAGMN_00705: mdtK - multidrug efflux MATE transporter MdtK, at 118,739 to 120,112 mdtK NIAGMN_00710: ydhQ - Uncharacterized protein YdhQ, at 120,153 to 121,409 ydhQ NIAGMN_00715: Val_trna - tRNA-Val(gac), at 121,717 to 121,793 _trna NIAGMN_00720: Val_trna - tRNA-Val(gac), at 121,798 to 121,874 _trna NIAGMN_00730: ydhR - monooxygenase, at 121,982 to 122,287 ydhR Position (kb) 120 121 122Strain fitness (log2 ratio) -1 0 1 2 3at 119.586 kb on + strand, within mdtKat 119.881 kb on + strand, within mdtKat 119.903 kb on + strand, within mdtKat 120.006 kb on + strandat 120.252 kb on + strandat 120.531 kb on - strand, within ydhQat 120.792 kb on - strand, within ydhQat 121.167 kb on - strand, within ydhQat 121.326 kb on - strandat 121.415 kb on - strandat 121.415 kb on - strandat 121.452 kb on - strandat 121.452 kb on - strandat 121.452 kb on - strandat 121.535 kb on - strandat 121.554 kb on - strandat 121.708 kb on + strandat 121.708 kb on + strandat 121.708 kb on + strandat 121.724 kb on + strandat 121.724 kb on + strandat 121.724 kb on + strandat 122.408 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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119,586 + mdtK NIAGMN_00705 0.62 +0.3
119,881 + mdtK NIAGMN_00705 0.83 -1.3
119,903 + mdtK NIAGMN_00705 0.85 -0.7
120,006 + -1.1
120,252 + +0.7
120,531 - ydhQ NIAGMN_00710 0.30 +3.7
120,792 - ydhQ NIAGMN_00710 0.51 +0.3
121,167 - ydhQ NIAGMN_00710 0.81 +0.3
121,326 - +0.6
121,415 - +2.7
121,415 - +1.8
121,452 - +0.7
121,452 - +2.7
121,452 - +0.4
121,535 - +1.1
121,554 - +0.1
121,708 + +0.1
121,708 + +0.3
121,708 + -0.4
121,724 + +1.7
121,724 + +1.3
121,724 + +1.8
122,408 + -0.2

Or see this region's nucleotide sequence