Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00410

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfolM and ydgC overlap by 4 nucleotidesydgC and rstA are separated by 128 nucleotidesrstA and rstB are separated by 3 nucleotides NIAGMN_00400: folM - dihydromonapterin reductase, at 59,055 to 59,777 folM NIAGMN_00405: ydgC - Inner membrane protein YdgC, at 59,774 to 60,109 ydgC NIAGMN_00410: rstA - two-component system response regulator RstA, at 60,238 to 60,957 rstA NIAGMN_00415: rstB - two-component system sensor histidine kinase RstB, at 60,961 to 62,262 rstB Position (kb) 60 61Strain fitness (log2 ratio) -1 0 1at 59.357 kb on - strand, within folMat 59.423 kb on - strand, within folMat 59.537 kb on + strand, within folMat 59.588 kb on + strand, within folMat 59.713 kb on + strandat 59.797 kb on + strandat 59.798 kb on - strandat 59.878 kb on - strand, within ydgCat 60.170 kb on - strandat 60.312 kb on + strand, within rstAat 60.315 kb on - strand, within rstAat 60.315 kb on - strand, within rstAat 60.755 kb on + strand, within rstAat 61.450 kb on - strand, within rstBat 61.507 kb on - strand, within rstBat 61.507 kb on - strand, within rstBat 61.848 kb on - strand, within rstBat 61.894 kb on + strand, within rstB

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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59,357 - folM NIAGMN_00400 0.42 +1.1
59,423 - folM NIAGMN_00400 0.51 -1.5
59,537 + folM NIAGMN_00400 0.67 +0.7
59,588 + folM NIAGMN_00400 0.74 +0.1
59,713 + +1.2
59,797 + +0.4
59,798 - -0.7
59,878 - ydgC NIAGMN_00405 0.31 -0.7
60,170 - +1.7
60,312 + rstA NIAGMN_00410 0.10 +0.3
60,315 - rstA NIAGMN_00410 0.11 -0.1
60,315 - rstA NIAGMN_00410 0.11 -0.5
60,755 + rstA NIAGMN_00410 0.72 -0.9
61,450 - rstB NIAGMN_00415 0.38 +1.7
61,507 - rstB NIAGMN_00415 0.42 +1.1
61,507 - rstB NIAGMN_00415 0.42 -0.3
61,848 - rstB NIAGMN_00415 0.68 -1.3
61,894 + rstB NIAGMN_00415 0.72 +1.7

Or see this region's nucleotide sequence