Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00355

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynfM and asr are separated by 423 nucleotidesasr and ydgU are separated by 92 nucleotidesydgU and ydgD are separated by 99 nucleotidesydgD and mdtI are separated by 38 nucleotides NIAGMN_00345: ynfM - Inner membrane transport protein YnfM, at 47,778 to 49,031 ynfM NIAGMN_00350: asr - acid resistance repetitive basic protein Asr, at 49,455 to 49,763 asr NIAGMN_00355: ydgU - Uncharacterized protein YdgU, at 49,856 to 49,939 ydgU NIAGMN_00360: ydgD - Uncharacterized serine protease YdgD, at 50,039 to 50,860 ydgD NIAGMN_00365: mdtI - multidrug/spermidine efflux SMR transporter subunit MdtI, at 50,899 to 51,228 mdtI Position (kb) 49 50Strain fitness (log2 ratio) -1 0 1 2 3at 48.889 kb on - strand, within ynfMat 48.935 kb on + strandat 48.935 kb on + strandat 48.935 kb on + strandat 49.071 kb on - strandat 49.096 kb on - strandat 49.158 kb on + strandat 49.160 kb on + strandat 49.160 kb on + strandat 49.161 kb on - strandat 49.165 kb on - strandat 49.165 kb on - strandat 49.302 kb on - strandat 49.338 kb on + strandat 49.396 kb on + strandat 49.397 kb on - strandat 49.465 kb on + strandat 49.985 kb on + strandat 50.045 kb on - strandat 50.449 kb on - strand, within ydgDat 50.494 kb on + strand, within ydgDat 50.495 kb on - strand, within ydgDat 50.624 kb on + strand, within ydgDat 50.624 kb on + strand, within ydgD

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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48,889 - ynfM NIAGMN_00345 0.89 -0.1
48,935 + +0.1
48,935 + +0.2
48,935 + -0.9
49,071 - +1.1
49,096 - -0.9
49,158 + -0.2
49,160 + +1.3
49,160 + -0.6
49,161 - -0.3
49,165 - +0.7
49,165 - -0.9
49,302 - +0.7
49,338 + +0.1
49,396 + -0.5
49,397 - +0.2
49,465 + -0.6
49,985 + +3.0
50,045 - +0.5
50,449 - ydgD NIAGMN_00360 0.50 -0.6
50,494 + ydgD NIAGMN_00360 0.55 +0.9
50,495 - ydgD NIAGMN_00360 0.55 -0.2
50,624 + ydgD NIAGMN_00360 0.71 +0.5
50,624 + ydgD NIAGMN_00360 0.71 +0.1

Or see this region's nucleotide sequence