Strain Fitness in Escherichia coli ECRC101 around MCAODC_22455

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntroxA and pepT are separated by 48 nucleotidespepT and potA are separated by 249 nucleotides MCAODC_22450: roxA - [50S ribosomal protein L16]-arginine 3-hydroxylase, at 1,248,644 to 1,249,765 roxA MCAODC_22455: pepT - peptidase T, at 1,249,814 to 1,251,040 pepT MCAODC_22460: potA - spermidine/putrescine ABC transporter ATP-binding protein PotA, at 1,251,290 to 1,252,426 potA Position (kb) 1249 1250 1251 1252Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1248.854 kb on + strand, within roxAat 1248.874 kb on + strand, within roxAat 1248.875 kb on - strand, within roxAat 1248.875 kb on - strand, within roxAat 1248.889 kb on - strand, within roxAat 1248.952 kb on - strand, within roxAat 1249.028 kb on + strand, within roxAat 1249.039 kb on + strand, within roxAat 1249.181 kb on + strand, within roxAat 1249.182 kb on - strand, within roxAat 1249.183 kb on + strand, within roxAat 1249.183 kb on + strand, within roxAat 1249.235 kb on + strand, within roxAat 1249.236 kb on - strand, within roxAat 1249.273 kb on - strand, within roxAat 1249.322 kb on + strand, within roxAat 1249.323 kb on - strand, within roxAat 1249.323 kb on - strand, within roxAat 1249.323 kb on - strand, within roxAat 1249.325 kb on + strand, within roxAat 1249.326 kb on - strand, within roxAat 1249.456 kb on + strand, within roxAat 1249.457 kb on - strand, within roxAat 1249.524 kb on - strand, within roxAat 1249.576 kb on - strand, within roxAat 1249.598 kb on + strand, within roxAat 1249.844 kb on - strandat 1249.900 kb on + strandat 1249.901 kb on - strandat 1249.909 kb on - strandat 1249.909 kb on - strandat 1249.944 kb on - strand, within pepTat 1250.048 kb on - strand, within pepTat 1250.078 kb on - strand, within pepTat 1250.082 kb on + strand, within pepTat 1250.083 kb on - strand, within pepTat 1250.083 kb on - strand, within pepTat 1250.085 kb on + strand, within pepTat 1250.086 kb on - strand, within pepTat 1250.086 kb on - strand, within pepTat 1250.113 kb on - strand, within pepTat 1250.127 kb on - strand, within pepTat 1250.188 kb on + strand, within pepTat 1250.211 kb on + strand, within pepTat 1250.212 kb on - strand, within pepTat 1250.212 kb on - strand, within pepTat 1250.272 kb on - strand, within pepTat 1250.272 kb on - strand, within pepTat 1250.321 kb on - strand, within pepTat 1250.464 kb on - strand, within pepTat 1250.631 kb on - strand, within pepTat 1250.641 kb on + strand, within pepTat 1250.642 kb on - strand, within pepTat 1250.696 kb on - strand, within pepTat 1250.735 kb on - strand, within pepTat 1250.939 kb on + strandat 1251.042 kb on + strandat 1251.071 kb on - strandat 1251.073 kb on + strandat 1251.094 kb on + strandat 1251.131 kb on + strandat 1251.132 kb on - strandat 1251.132 kb on - strandat 1251.136 kb on - strandat 1251.144 kb on + strandat 1251.165 kb on - strandat 1251.217 kb on + strandat 1251.217 kb on + strandat 1251.229 kb on + strandat 1251.229 kb on + strandat 1251.270 kb on - strandat 1251.581 kb on + strand, within potAat 1251.581 kb on + strand, within potAat 1251.886 kb on + strand, within potAat 1251.887 kb on - strand, within potA

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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1,248,854 + roxA MCAODC_22450 0.19 -1.7
1,248,874 + roxA MCAODC_22450 0.20 -0.7
1,248,875 - roxA MCAODC_22450 0.21 +1.6
1,248,875 - roxA MCAODC_22450 0.21 +0.0
1,248,889 - roxA MCAODC_22450 0.22 -0.4
1,248,952 - roxA MCAODC_22450 0.27 +0.6
1,249,028 + roxA MCAODC_22450 0.34 -1.2
1,249,039 + roxA MCAODC_22450 0.35 +1.8
1,249,181 + roxA MCAODC_22450 0.48 -0.4
1,249,182 - roxA MCAODC_22450 0.48 +0.3
1,249,183 + roxA MCAODC_22450 0.48 -1.1
1,249,183 + roxA MCAODC_22450 0.48 +0.8
1,249,235 + roxA MCAODC_22450 0.53 +0.6
1,249,236 - roxA MCAODC_22450 0.53 -0.5
1,249,273 - roxA MCAODC_22450 0.56 +2.0
1,249,322 + roxA MCAODC_22450 0.60 +0.4
1,249,323 - roxA MCAODC_22450 0.61 -0.4
1,249,323 - roxA MCAODC_22450 0.61 +1.6
1,249,323 - roxA MCAODC_22450 0.61 -0.4
1,249,325 + roxA MCAODC_22450 0.61 +1.6
1,249,326 - roxA MCAODC_22450 0.61 +0.0
1,249,456 + roxA MCAODC_22450 0.72 -0.4
1,249,457 - roxA MCAODC_22450 0.72 -0.7
1,249,524 - roxA MCAODC_22450 0.78 +0.4
1,249,576 - roxA MCAODC_22450 0.83 -0.3
1,249,598 + roxA MCAODC_22450 0.85 -1.9
1,249,844 - -0.2
1,249,900 + +1.2
1,249,901 - +0.0
1,249,909 - +0.7
1,249,909 - -0.1
1,249,944 - pepT MCAODC_22455 0.11 +1.2
1,250,048 - pepT MCAODC_22455 0.19 -2.9
1,250,078 - pepT MCAODC_22455 0.22 +1.0
1,250,082 + pepT MCAODC_22455 0.22 -0.4
1,250,083 - pepT MCAODC_22455 0.22 +0.2
1,250,083 - pepT MCAODC_22455 0.22 +0.3
1,250,085 + pepT MCAODC_22455 0.22 -0.7
1,250,086 - pepT MCAODC_22455 0.22 -1.5
1,250,086 - pepT MCAODC_22455 0.22 +0.8
1,250,113 - pepT MCAODC_22455 0.24 -0.5
1,250,127 - pepT MCAODC_22455 0.26 +0.2
1,250,188 + pepT MCAODC_22455 0.30 -1.0
1,250,211 + pepT MCAODC_22455 0.32 +0.0
1,250,212 - pepT MCAODC_22455 0.32 +2.6
1,250,212 - pepT MCAODC_22455 0.32 -1.4
1,250,272 - pepT MCAODC_22455 0.37 +1.0
1,250,272 - pepT MCAODC_22455 0.37 +1.3
1,250,321 - pepT MCAODC_22455 0.41 +0.6
1,250,464 - pepT MCAODC_22455 0.53 +0.0
1,250,631 - pepT MCAODC_22455 0.67 -0.9
1,250,641 + pepT MCAODC_22455 0.67 +1.6
1,250,642 - pepT MCAODC_22455 0.67 +0.1
1,250,696 - pepT MCAODC_22455 0.72 +0.6
1,250,735 - pepT MCAODC_22455 0.75 +1.7
1,250,939 + -1.0
1,251,042 + +0.6
1,251,071 - +0.0
1,251,073 + -2.2
1,251,094 + -0.7
1,251,131 + +1.6
1,251,132 - -0.1
1,251,132 - -0.7
1,251,136 - +0.4
1,251,144 + -2.9
1,251,165 - -0.4
1,251,217 + +1.6
1,251,217 + -1.1
1,251,229 + -1.6
1,251,229 + +1.6
1,251,270 - -2.0
1,251,581 + potA MCAODC_22460 0.26 -0.4
1,251,581 + potA MCAODC_22460 0.26 -0.5
1,251,886 + potA MCAODC_22460 0.52 -0.8
1,251,887 - potA MCAODC_22460 0.53 -1.0

Or see this region's nucleotide sequence