Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt roxA and pepT are separated by 48 nucleotides pepT and potA are separated by 249 nucleotides
MCAODC_22450: roxA - [50S ribosomal protein L16]-arginine 3-hydroxylase, at 1,248,644 to 1,249,765
roxA
MCAODC_22455: pepT - peptidase T, at 1,249,814 to 1,251,040
pepT
MCAODC_22460: potA - spermidine/putrescine ABC transporter ATP-binding protein PotA, at 1,251,290 to 1,252,426
potA
Position (kb)
1249
1250
1251
1252 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1248.854 kb on + strand, within roxA at 1248.874 kb on + strand, within roxA at 1248.875 kb on - strand, within roxA at 1248.875 kb on - strand, within roxA at 1248.889 kb on - strand, within roxA at 1248.952 kb on - strand, within roxA at 1249.028 kb on + strand, within roxA at 1249.039 kb on + strand, within roxA at 1249.181 kb on + strand, within roxA at 1249.182 kb on - strand, within roxA at 1249.183 kb on + strand, within roxA at 1249.183 kb on + strand, within roxA at 1249.235 kb on + strand, within roxA at 1249.236 kb on - strand, within roxA at 1249.273 kb on - strand, within roxA at 1249.322 kb on + strand, within roxA at 1249.323 kb on - strand, within roxA at 1249.323 kb on - strand, within roxA at 1249.323 kb on - strand, within roxA at 1249.325 kb on + strand, within roxA at 1249.326 kb on - strand, within roxA at 1249.456 kb on + strand, within roxA at 1249.457 kb on - strand, within roxA at 1249.524 kb on - strand, within roxA at 1249.576 kb on - strand, within roxA at 1249.598 kb on + strand, within roxA at 1249.844 kb on - strand at 1249.900 kb on + strand at 1249.901 kb on - strand at 1249.909 kb on - strand at 1249.909 kb on - strand at 1249.944 kb on - strand, within pepT at 1250.048 kb on - strand, within pepT at 1250.078 kb on - strand, within pepT at 1250.082 kb on + strand, within pepT at 1250.083 kb on - strand, within pepT at 1250.083 kb on - strand, within pepT at 1250.085 kb on + strand, within pepT at 1250.086 kb on - strand, within pepT at 1250.086 kb on - strand, within pepT at 1250.113 kb on - strand, within pepT at 1250.127 kb on - strand, within pepT at 1250.188 kb on + strand, within pepT at 1250.211 kb on + strand, within pepT at 1250.212 kb on - strand, within pepT at 1250.212 kb on - strand, within pepT at 1250.272 kb on - strand, within pepT at 1250.272 kb on - strand, within pepT at 1250.321 kb on - strand, within pepT at 1250.464 kb on - strand, within pepT at 1250.631 kb on - strand, within pepT at 1250.641 kb on + strand, within pepT at 1250.642 kb on - strand, within pepT at 1250.696 kb on - strand, within pepT at 1250.735 kb on - strand, within pepT at 1250.939 kb on + strand at 1251.042 kb on + strand at 1251.071 kb on - strand at 1251.073 kb on + strand at 1251.094 kb on + strand at 1251.131 kb on + strand at 1251.132 kb on - strand at 1251.132 kb on - strand at 1251.136 kb on - strand at 1251.144 kb on + strand at 1251.165 kb on - strand at 1251.217 kb on + strand at 1251.217 kb on + strand at 1251.229 kb on + strand at 1251.229 kb on + strand at 1251.270 kb on - strand at 1251.581 kb on + strand, within potA at 1251.581 kb on + strand, within potA at 1251.886 kb on + strand, within potA at 1251.887 kb on - strand, within potA
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 1,248,854 + roxA MCAODC_22450 0.19 -1.7 1,248,874 + roxA MCAODC_22450 0.20 -0.7 1,248,875 - roxA MCAODC_22450 0.21 +1.6 1,248,875 - roxA MCAODC_22450 0.21 +0.0 1,248,889 - roxA MCAODC_22450 0.22 -0.4 1,248,952 - roxA MCAODC_22450 0.27 +0.6 1,249,028 + roxA MCAODC_22450 0.34 -1.2 1,249,039 + roxA MCAODC_22450 0.35 +1.8 1,249,181 + roxA MCAODC_22450 0.48 -0.4 1,249,182 - roxA MCAODC_22450 0.48 +0.3 1,249,183 + roxA MCAODC_22450 0.48 -1.1 1,249,183 + roxA MCAODC_22450 0.48 +0.8 1,249,235 + roxA MCAODC_22450 0.53 +0.6 1,249,236 - roxA MCAODC_22450 0.53 -0.5 1,249,273 - roxA MCAODC_22450 0.56 +2.0 1,249,322 + roxA MCAODC_22450 0.60 +0.4 1,249,323 - roxA MCAODC_22450 0.61 -0.4 1,249,323 - roxA MCAODC_22450 0.61 +1.6 1,249,323 - roxA MCAODC_22450 0.61 -0.4 1,249,325 + roxA MCAODC_22450 0.61 +1.6 1,249,326 - roxA MCAODC_22450 0.61 +0.0 1,249,456 + roxA MCAODC_22450 0.72 -0.4 1,249,457 - roxA MCAODC_22450 0.72 -0.7 1,249,524 - roxA MCAODC_22450 0.78 +0.4 1,249,576 - roxA MCAODC_22450 0.83 -0.3 1,249,598 + roxA MCAODC_22450 0.85 -1.9 1,249,844 - -0.2 1,249,900 + +1.2 1,249,901 - +0.0 1,249,909 - +0.7 1,249,909 - -0.1 1,249,944 - pepT MCAODC_22455 0.11 +1.2 1,250,048 - pepT MCAODC_22455 0.19 -2.9 1,250,078 - pepT MCAODC_22455 0.22 +1.0 1,250,082 + pepT MCAODC_22455 0.22 -0.4 1,250,083 - pepT MCAODC_22455 0.22 +0.2 1,250,083 - pepT MCAODC_22455 0.22 +0.3 1,250,085 + pepT MCAODC_22455 0.22 -0.7 1,250,086 - pepT MCAODC_22455 0.22 -1.5 1,250,086 - pepT MCAODC_22455 0.22 +0.8 1,250,113 - pepT MCAODC_22455 0.24 -0.5 1,250,127 - pepT MCAODC_22455 0.26 +0.2 1,250,188 + pepT MCAODC_22455 0.30 -1.0 1,250,211 + pepT MCAODC_22455 0.32 +0.0 1,250,212 - pepT MCAODC_22455 0.32 +2.6 1,250,212 - pepT MCAODC_22455 0.32 -1.4 1,250,272 - pepT MCAODC_22455 0.37 +1.0 1,250,272 - pepT MCAODC_22455 0.37 +1.3 1,250,321 - pepT MCAODC_22455 0.41 +0.6 1,250,464 - pepT MCAODC_22455 0.53 +0.0 1,250,631 - pepT MCAODC_22455 0.67 -0.9 1,250,641 + pepT MCAODC_22455 0.67 +1.6 1,250,642 - pepT MCAODC_22455 0.67 +0.1 1,250,696 - pepT MCAODC_22455 0.72 +0.6 1,250,735 - pepT MCAODC_22455 0.75 +1.7 1,250,939 + -1.0 1,251,042 + +0.6 1,251,071 - +0.0 1,251,073 + -2.2 1,251,094 + -0.7 1,251,131 + +1.6 1,251,132 - -0.1 1,251,132 - -0.7 1,251,136 - +0.4 1,251,144 + -2.9 1,251,165 - -0.4 1,251,217 + +1.6 1,251,217 + -1.1 1,251,229 + -1.6 1,251,229 + +1.6 1,251,270 - -2.0 1,251,581 + potA MCAODC_22460 0.26 -0.4 1,251,581 + potA MCAODC_22460 0.26 -0.5 1,251,886 + potA MCAODC_22460 0.52 -0.8 1,251,887 - potA MCAODC_22460 0.53 -1.0
Or see this region's nucleotide sequence