Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdsA and sirB1 are separated by 35 nucleotides sirB1 and sirB2 are separated by 3 nucleotides sirB2 and prmC overlap by 4 nucleotides prmC and prfA overlap by 1 nucleotides
MCAODC_21790: kdsA - 3-deoxy-8-phosphooctulonate synthase, at 1,140,953 to 1,141,807
kdsA
MCAODC_21795: sirB1 - invasion regulator SirB1, at 1,141,843 to 1,142,652
sirB1
MCAODC_21800: sirB2 - invasion regulator SirB2, at 1,142,656 to 1,143,048
sirB2
MCAODC_21805: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 1,143,045 to 1,143,878
prmC
MCAODC_21810: prfA - peptide chain release factor 1, at 1,143,878 to 1,144,960
prfA
Position (kb)
1142
1143
1144 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1141.845 kb on - strand at 1141.858 kb on - strand at 1141.876 kb on - strand at 1141.876 kb on - strand at 1141.876 kb on - strand at 1141.940 kb on - strand, within sirB1 at 1141.940 kb on - strand, within sirB1 at 1141.940 kb on - strand, within sirB1 at 1142.033 kb on - strand, within sirB1 at 1142.117 kb on - strand, within sirB1 at 1142.151 kb on - strand, within sirB1 at 1142.192 kb on - strand, within sirB1 at 1142.203 kb on - strand, within sirB1 at 1142.350 kb on + strand, within sirB1 at 1142.351 kb on - strand, within sirB1 at 1142.351 kb on - strand, within sirB1 at 1142.692 kb on - strand at 1142.692 kb on - strand at 1142.702 kb on + strand, within sirB2 at 1142.703 kb on - strand, within sirB2 at 1142.703 kb on - strand, within sirB2 at 1142.779 kb on - strand, within sirB2 at 1142.796 kb on - strand, within sirB2 at 1142.796 kb on - strand, within sirB2 at 1142.803 kb on - strand, within sirB2 at 1142.893 kb on + strand, within sirB2 at 1142.894 kb on - strand, within sirB2 at 1142.966 kb on - strand, within sirB2 at 1142.978 kb on - strand, within sirB2 at 1142.984 kb on - strand, within sirB2 at 1142.984 kb on - strand, within sirB2 at 1143.012 kb on + strand at 1143.013 kb on - strand at 1143.013 kb on - strand at 1143.013 kb on - strand at 1143.013 kb on - strand at 1143.013 kb on - strand at 1143.013 kb on - strand at 1143.426 kb on - strand, within prmC at 1143.667 kb on - strand, within prmC at 1143.765 kb on - strand, within prmC at 1143.929 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 1,141,845 - +0.5 1,141,858 - +1.5 1,141,876 - -1.2 1,141,876 - -0.7 1,141,876 - -0.2 1,141,940 - sirB1 MCAODC_21795 0.12 +0.0 1,141,940 - sirB1 MCAODC_21795 0.12 -0.7 1,141,940 - sirB1 MCAODC_21795 0.12 +0.5 1,142,033 - sirB1 MCAODC_21795 0.23 -1.0 1,142,117 - sirB1 MCAODC_21795 0.34 -0.1 1,142,151 - sirB1 MCAODC_21795 0.38 +1.6 1,142,192 - sirB1 MCAODC_21795 0.43 +0.6 1,142,203 - sirB1 MCAODC_21795 0.44 +0.8 1,142,350 + sirB1 MCAODC_21795 0.63 +1.0 1,142,351 - sirB1 MCAODC_21795 0.63 +0.0 1,142,351 - sirB1 MCAODC_21795 0.63 +1.6 1,142,692 - +0.6 1,142,692 - +0.3 1,142,702 + sirB2 MCAODC_21800 0.12 +0.9 1,142,703 - sirB2 MCAODC_21800 0.12 +1.0 1,142,703 - sirB2 MCAODC_21800 0.12 -0.4 1,142,779 - sirB2 MCAODC_21800 0.31 +2.6 1,142,796 - sirB2 MCAODC_21800 0.36 -1.2 1,142,796 - sirB2 MCAODC_21800 0.36 +2.4 1,142,803 - sirB2 MCAODC_21800 0.37 +1.0 1,142,893 + sirB2 MCAODC_21800 0.60 +0.0 1,142,894 - sirB2 MCAODC_21800 0.61 -1.0 1,142,966 - sirB2 MCAODC_21800 0.79 +2.6 1,142,978 - sirB2 MCAODC_21800 0.82 -1.0 1,142,984 - sirB2 MCAODC_21800 0.83 +1.6 1,142,984 - sirB2 MCAODC_21800 0.83 -1.0 1,143,012 + -0.6 1,143,013 - +1.0 1,143,013 - +1.0 1,143,013 - -1.4 1,143,013 - -2.4 1,143,013 - +0.6 1,143,013 - +4.0 1,143,426 - prmC MCAODC_21805 0.46 +1.2 1,143,667 - prmC MCAODC_21805 0.75 +1.6 1,143,765 - prmC MCAODC_21805 0.86 +1.6 1,143,929 - -0.7
Or see this region's nucleotide sequence