Strain Fitness in Escherichia coli ECRC101 around MCAODC_21500

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_21490 and MCAODC_21495 overlap by 4 nucleotidesMCAODC_21495 and MCAODC_21500 are separated by 186 nucleotidesMCAODC_21500 and eCs1768 are separated by 31 nucleotides MCAODC_21490: MCAODC_21490 - hypothetical protein, at 1,088,249 to 1,088,413 _21490 MCAODC_21495: MCAODC_21495 - DNA-binding protein, at 1,088,410 to 1,088,991 _21495 MCAODC_21500: MCAODC_21500 - replication protein, at 1,089,178 to 1,089,600 _21500 MCAODC_21505: eCs1768 - DNA-binding protein, at 1,089,632 to 1,090,672 eCs1768 Position (kb) 1089 1090Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1088.188 kb on - strandat 1088.188 kb on - strandat 1088.191 kb on + strandat 1088.191 kb on + strandat 1088.192 kb on - strandat 1088.192 kb on - strandat 1088.367 kb on - strand, within MCAODC_21490at 1088.379 kb on - strand, within MCAODC_21490at 1088.379 kb on - strand, within MCAODC_21490at 1088.382 kb on - strand, within MCAODC_21490at 1088.625 kb on + strand, within MCAODC_21495at 1088.740 kb on - strand, within MCAODC_21495at 1089.002 kb on + strandat 1089.210 kb on - strandat 1089.210 kb on - strandat 1089.301 kb on - strand, within MCAODC_21500at 1089.304 kb on - strand, within MCAODC_21500at 1089.304 kb on - strand, within MCAODC_21500at 1089.304 kb on - strand, within MCAODC_21500at 1089.434 kb on + strand, within MCAODC_21500at 1089.439 kb on - strand, within MCAODC_21500at 1089.439 kb on - strand, within MCAODC_21500at 1089.890 kb on + strand, within eCs1768at 1089.891 kb on - strand, within eCs1768at 1089.891 kb on - strand, within eCs1768at 1089.955 kb on - strand, within eCs1768at 1089.986 kb on - strand, within eCs1768at 1090.107 kb on - strand, within eCs1768at 1090.172 kb on - strand, within eCs1768at 1090.185 kb on - strand, within eCs1768at 1090.409 kb on + strand, within eCs1768at 1090.554 kb on - strand, within eCs1768at 1090.554 kb on - strand, within eCs1768at 1090.588 kb on + strandat 1090.589 kb on - strandat 1090.589 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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1,088,188 - +1.7
1,088,188 - +0.3
1,088,191 + -1.9
1,088,191 + +3.2
1,088,192 - +0.4
1,088,192 - +0.1
1,088,367 - MCAODC_21490 0.72 +0.2
1,088,379 - MCAODC_21490 0.79 +0.2
1,088,379 - MCAODC_21490 0.79 +0.7
1,088,382 - MCAODC_21490 0.81 -0.3
1,088,625 + MCAODC_21495 0.37 -0.2
1,088,740 - MCAODC_21495 0.57 +0.1
1,089,002 + -1.3
1,089,210 - +1.7
1,089,210 - +0.1
1,089,301 - MCAODC_21500 0.29 -0.5
1,089,304 - MCAODC_21500 0.30 +0.7
1,089,304 - MCAODC_21500 0.30 +0.2
1,089,304 - MCAODC_21500 0.30 -0.9
1,089,434 + MCAODC_21500 0.61 -0.9
1,089,439 - MCAODC_21500 0.62 +1.3
1,089,439 - MCAODC_21500 0.62 +1.7
1,089,890 + eCs1768 MCAODC_21505 0.25 +1.7
1,089,891 - eCs1768 MCAODC_21505 0.25 +1.2
1,089,891 - eCs1768 MCAODC_21505 0.25 +0.2
1,089,955 - eCs1768 MCAODC_21505 0.31 -0.3
1,089,986 - eCs1768 MCAODC_21505 0.34 +1.5
1,090,107 - eCs1768 MCAODC_21505 0.46 +0.7
1,090,172 - eCs1768 MCAODC_21505 0.52 -0.2
1,090,185 - eCs1768 MCAODC_21505 0.53 -0.0
1,090,409 + eCs1768 MCAODC_21505 0.75 -0.3
1,090,554 - eCs1768 MCAODC_21505 0.89 -1.0
1,090,554 - eCs1768 MCAODC_21505 0.89 -2.0
1,090,588 + +0.7
1,090,589 - +0.7
1,090,589 - +0.4

Or see this region's nucleotide sequence