Strain Fitness in Escherichia coli ECRC101 around MCAODC_20490

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmanC2 and wbdQ overlap by 19 nucleotideswbdQ and wcaG are separated by 2 nucleotideswcaG and MCAODC_20500 are separated by 2 nucleotides MCAODC_20485: manC2 - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 904,724 to 906,172 manC2 MCAODC_20490: wbdQ - GDP-mannose mannosyl hydrolase WbdQ/WbhG, at 906,154 to 906,663 wbdQ MCAODC_20495: wcaG - GDP-fucose synthetase, at 906,666 to 907,631 wcaG MCAODC_20500: MCAODC_20500 - GDP-mannose 4,6-dehydratase, at 907,634 to 908,752 _20500 Position (kb) 906 907Strain fitness (log2 ratio) -2 -1 0 1at 906.176 kb on - strandat 906.350 kb on - strand, within wbdQat 906.534 kb on - strand, within wbdQat 906.991 kb on - strand, within wcaGat 907.226 kb on - strand, within wcaG

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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906,176 - -2.4
906,350 - wbdQ MCAODC_20490 0.38 -2.4
906,534 - wbdQ MCAODC_20490 0.75 +0.1
906,991 - wcaG MCAODC_20495 0.34 -1.1
907,226 - wcaG MCAODC_20495 0.58 -1.3

Or see this region's nucleotide sequence