Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fnr and ogt are separated by 194 nucleotides ogt and abgT are separated by 10 nucleotides abgT and abgB are separated by 36 nucleotides
MCAODC_16030: fnr - fumarate/nitrate reduction transcriptional regulator Fnr, at 96,921 to 97,673
fnr
MCAODC_16035: ogt - methylated-DNA--[protein]-cysteine S-methyltransferase, at 97,868 to 98,383
ogt
MCAODC_16040: abgT - p-aminobenzoyl-glutamate transporter, at 98,394 to 99,920
abgT
MCAODC_16045: abgB - p-aminobenzoyl-glutamate hydrolase subunit AbgB, at 99,957 to 101,402
abgB
Position (kb) 98
99
100 Strain fitness (log2 ratio) -2
-1
0
1
2 at 97.439 kb on - strand, within fnr at 97.439 kb on - strand, within fnr at 97.439 kb on - strand, within fnr at 97.479 kb on - strand, within fnr at 97.545 kb on - strand, within fnr at 97.545 kb on - strand, within fnr at 97.622 kb on + strand at 97.673 kb on + strand at 97.674 kb on - strand at 97.674 kb on - strand at 97.691 kb on - strand at 97.732 kb on + strand at 97.733 kb on - strand at 97.782 kb on - strand at 97.782 kb on - strand at 97.784 kb on - strand at 97.784 kb on - strand at 97.784 kb on - strand at 97.863 kb on - strand at 97.929 kb on + strand, within ogt at 98.069 kb on + strand, within ogt at 98.233 kb on - strand, within ogt at 98.284 kb on - strand, within ogt at 98.410 kb on + strand at 98.444 kb on - strand at 98.470 kb on - strand at 98.512 kb on + strand at 98.512 kb on + strand at 98.513 kb on - strand at 98.513 kb on - strand at 98.540 kb on - strand at 98.599 kb on - strand, within abgT at 98.616 kb on - strand, within abgT at 98.655 kb on + strand, within abgT at 98.661 kb on - strand, within abgT at 98.840 kb on + strand, within abgT at 99.011 kb on + strand, within abgT at 99.129 kb on + strand, within abgT at 99.129 kb on + strand, within abgT at 99.278 kb on - strand, within abgT at 99.285 kb on - strand, within abgT at 99.292 kb on - strand, within abgT at 99.376 kb on - strand, within abgT at 99.524 kb on + strand, within abgT at 99.525 kb on - strand, within abgT at 99.537 kb on - strand, within abgT at 99.537 kb on - strand, within abgT at 99.537 kb on - strand, within abgT at 99.537 kb on - strand, within abgT at 99.538 kb on + strand, within abgT at 99.539 kb on - strand, within abgT at 99.558 kb on - strand, within abgT at 99.582 kb on + strand, within abgT at 99.677 kb on + strand, within abgT at 99.678 kb on - strand, within abgT at 99.678 kb on - strand, within abgT at 99.805 kb on - strand at 99.817 kb on + strand at 99.821 kb on + strand at 99.875 kb on - strand at 99.914 kb on + strand at 99.914 kb on + strand at 99.992 kb on + strand at 100.003 kb on + strand at 100.003 kb on + strand at 100.019 kb on + strand at 100.054 kb on - strand at 100.130 kb on + strand, within abgB at 100.130 kb on + strand, within abgB at 100.131 kb on - strand, within abgB at 100.163 kb on + strand, within abgB at 100.163 kb on + strand, within abgB at 100.163 kb on + strand, within abgB at 100.163 kb on + strand, within abgB at 100.163 kb on + strand, within abgB at 100.163 kb on + strand, within abgB at 100.163 kb on + strand, within abgB at 100.163 kb on + strand, within abgB at 100.163 kb on + strand, within abgB at 100.164 kb on - strand, within abgB at 100.164 kb on - strand, within abgB at 100.167 kb on + strand, within abgB at 100.175 kb on + strand, within abgB at 100.277 kb on - strand, within abgB at 100.317 kb on + strand, within abgB at 100.318 kb on - strand, within abgB at 100.392 kb on - strand, within abgB at 100.471 kb on + strand, within abgB at 100.471 kb on + strand, within abgB at 100.471 kb on + strand, within abgB at 100.472 kb on - strand, within abgB at 100.591 kb on + strand, within abgB at 100.591 kb on + strand, within abgB at 100.633 kb on + strand, within abgB at 100.682 kb on + strand, within abgB at 100.696 kb on + strand, within abgB at 100.696 kb on + strand, within abgB at 100.697 kb on - strand, within abgB at 100.702 kb on - strand, within abgB at 100.721 kb on + strand, within abgB at 100.722 kb on - strand, within abgB at 100.722 kb on - strand, within abgB at 100.722 kb on - strand, within abgB
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 97,439 - fnr MCAODC_16030 0.69 -1.2 97,439 - fnr MCAODC_16030 0.69 -1.4 97,439 - fnr MCAODC_16030 0.69 -1.2 97,479 - fnr MCAODC_16030 0.74 -1.8 97,545 - fnr MCAODC_16030 0.83 -0.8 97,545 - fnr MCAODC_16030 0.83 +0.0 97,622 + -0.7 97,673 + -1.8 97,674 - -1.4 97,674 - +0.2 97,691 - -0.4 97,732 + +0.6 97,733 - -0.4 97,782 - +0.0 97,782 - -0.1 97,784 - +1.0 97,784 - +1.6 97,784 - +0.6 97,863 - +0.0 97,929 + ogt MCAODC_16035 0.12 +0.3 98,069 + ogt MCAODC_16035 0.39 +0.6 98,233 - ogt MCAODC_16035 0.71 -0.4 98,284 - ogt MCAODC_16035 0.81 -0.4 98,410 + +0.0 98,444 - +1.6 98,470 - +1.4 98,512 + +1.0 98,512 + -1.4 98,513 - +0.6 98,513 - +2.5 98,540 - +0.9 98,599 - abgT MCAODC_16040 0.13 -1.0 98,616 - abgT MCAODC_16040 0.15 -0.8 98,655 + abgT MCAODC_16040 0.17 +0.6 98,661 - abgT MCAODC_16040 0.17 +0.1 98,840 + abgT MCAODC_16040 0.29 -0.3 99,011 + abgT MCAODC_16040 0.40 -0.5 99,129 + abgT MCAODC_16040 0.48 +1.6 99,129 + abgT MCAODC_16040 0.48 +1.6 99,278 - abgT MCAODC_16040 0.58 -0.2 99,285 - abgT MCAODC_16040 0.58 -0.1 99,292 - abgT MCAODC_16040 0.59 +0.3 99,376 - abgT MCAODC_16040 0.64 -1.8 99,524 + abgT MCAODC_16040 0.74 +0.0 99,525 - abgT MCAODC_16040 0.74 -0.7 99,537 - abgT MCAODC_16040 0.75 +2.2 99,537 - abgT MCAODC_16040 0.75 +0.6 99,537 - abgT MCAODC_16040 0.75 -1.0 99,537 - abgT MCAODC_16040 0.75 +1.6 99,538 + abgT MCAODC_16040 0.75 -1.4 99,539 - abgT MCAODC_16040 0.75 +0.0 99,558 - abgT MCAODC_16040 0.76 -1.0 99,582 + abgT MCAODC_16040 0.78 +0.0 99,677 + abgT MCAODC_16040 0.84 -1.0 99,678 - abgT MCAODC_16040 0.84 +1.1 99,678 - abgT MCAODC_16040 0.84 -0.1 99,805 - -0.9 99,817 + +1.6 99,821 + -1.2 99,875 - +1.6 99,914 + +0.9 99,914 + +0.8 99,992 + -0.1 100,003 + +0.6 100,003 + -0.7 100,019 + -0.4 100,054 - +0.0 100,130 + abgB MCAODC_16045 0.12 -0.1 100,130 + abgB MCAODC_16045 0.12 +0.7 100,131 - abgB MCAODC_16045 0.12 +0.2 100,163 + abgB MCAODC_16045 0.14 +0.0 100,163 + abgB MCAODC_16045 0.14 -0.1 100,163 + abgB MCAODC_16045 0.14 -2.2 100,163 + abgB MCAODC_16045 0.14 -0.7 100,163 + abgB MCAODC_16045 0.14 +0.5 100,163 + abgB MCAODC_16045 0.14 -1.5 100,163 + abgB MCAODC_16045 0.14 +0.3 100,163 + abgB MCAODC_16045 0.14 +1.2 100,163 + abgB MCAODC_16045 0.14 +0.6 100,164 - abgB MCAODC_16045 0.14 +0.6 100,164 - abgB MCAODC_16045 0.14 -0.7 100,167 + abgB MCAODC_16045 0.15 +0.6 100,175 + abgB MCAODC_16045 0.15 +0.5 100,277 - abgB MCAODC_16045 0.22 +0.5 100,317 + abgB MCAODC_16045 0.25 -0.1 100,318 - abgB MCAODC_16045 0.25 +1.0 100,392 - abgB MCAODC_16045 0.30 -1.1 100,471 + abgB MCAODC_16045 0.36 +1.0 100,471 + abgB MCAODC_16045 0.36 +0.6 100,471 + abgB MCAODC_16045 0.36 -1.2 100,472 - abgB MCAODC_16045 0.36 -0.4 100,591 + abgB MCAODC_16045 0.44 -1.5 100,591 + abgB MCAODC_16045 0.44 +1.6 100,633 + abgB MCAODC_16045 0.47 +0.6 100,682 + abgB MCAODC_16045 0.50 -0.4 100,696 + abgB MCAODC_16045 0.51 +0.6 100,696 + abgB MCAODC_16045 0.51 +0.0 100,697 - abgB MCAODC_16045 0.51 +0.6 100,702 - abgB MCAODC_16045 0.52 +0.3 100,721 + abgB MCAODC_16045 0.53 -1.8 100,722 - abgB MCAODC_16045 0.53 -0.5 100,722 - abgB MCAODC_16045 0.53 +0.9 100,722 - abgB MCAODC_16045 0.53 +0.6
Or see this region's nucleotide sequence