Strain Fitness in Escherichia coli ECRC101 around MCAODC_00185

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntxerD and Thr_trna are separated by 14 nucleotidesThr_trna and proA are separated by 114 nucleotides MCAODC_00180: xerD - Site-specific recombinase XerD, at 13,548 to 14,522 xerD MCAODC_00185: Thr_trna - tRNA-Thr(cgt), at 14,537 to 14,612 _trna MCAODC_00190: proA - glutamate-5-semialdehyde dehydrogenase, at 14,727 to 15,980 proA Position (kb) 14 15Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 13.642 kb on + strandat 13.642 kb on + strandat 13.642 kb on + strandat 13.643 kb on - strandat 13.643 kb on - strandat 13.653 kb on - strand, within xerDat 13.653 kb on - strand, within xerDat 13.764 kb on + strand, within xerDat 13.814 kb on + strand, within xerDat 13.814 kb on + strand, within xerDat 13.829 kb on - strand, within xerDat 13.877 kb on + strand, within xerDat 13.999 kb on + strand, within xerDat 14.000 kb on - strand, within xerDat 14.000 kb on - strand, within xerDat 14.038 kb on + strand, within xerDat 14.038 kb on + strand, within xerDat 14.038 kb on + strand, within xerDat 14.038 kb on + strand, within xerDat 14.038 kb on + strand, within xerDat 14.038 kb on + strand, within xerDat 14.039 kb on - strand, within xerDat 14.039 kb on - strand, within xerDat 14.042 kb on + strand, within xerDat 14.043 kb on - strand, within xerDat 14.043 kb on - strand, within xerDat 14.043 kb on - strand, within xerDat 14.082 kb on - strand, within xerDat 14.117 kb on + strand, within xerDat 14.167 kb on + strand, within xerDat 14.168 kb on - strand, within xerDat 14.168 kb on - strand, within xerDat 14.175 kb on + strand, within xerDat 14.359 kb on + strand, within xerDat 14.359 kb on + strand, within xerDat 14.360 kb on - strand, within xerDat 14.404 kb on - strand, within xerDat 14.420 kb on + strand, within xerDat 14.421 kb on - strand, within xerDat 14.421 kb on - strand, within xerDat 14.499 kb on - strandat 14.511 kb on - strandat 14.549 kb on + strand, within Thr_trnaat 14.605 kb on - strandat 14.767 kb on + strandat 14.767 kb on + strandat 14.768 kb on - strandat 14.768 kb on - strandat 14.768 kb on - strandat 14.823 kb on + strandat 15.056 kb on - strand, within proAat 15.129 kb on + strand, within proAat 15.129 kb on + strand, within proAat 15.180 kb on - strand, within proAat 15.180 kb on - strand, within proAat 15.181 kb on + strand, within proAat 15.213 kb on + strand, within proAat 15.219 kb on - strand, within proAat 15.219 kb on - strand, within proAat 15.219 kb on - strand, within proAat 15.221 kb on + strand, within proAat 15.221 kb on + strand, within proAat 15.221 kb on + strand, within proAat 15.221 kb on + strand, within proAat 15.221 kb on + strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.222 kb on - strand, within proAat 15.259 kb on - strand, within proAat 15.298 kb on + strand, within proAat 15.302 kb on + strand, within proAat 15.302 kb on + strand, within proAat 15.303 kb on - strand, within proAat 15.310 kb on - strand, within proAat 15.403 kb on + strand, within proAat 15.403 kb on + strand, within proAat 15.404 kb on - strand, within proAat 15.404 kb on - strand, within proAat 15.404 kb on - strand, within proAat 15.414 kb on - strand, within proAat 15.450 kb on - strand, within proA

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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13,642 + +2.7
13,642 + -0.3
13,642 + +0.5
13,643 - +0.2
13,643 - -1.4
13,653 - xerD MCAODC_00180 0.11 +0.6
13,653 - xerD MCAODC_00180 0.11 +0.4
13,764 + xerD MCAODC_00180 0.22 +0.2
13,814 + xerD MCAODC_00180 0.27 -1.5
13,814 + xerD MCAODC_00180 0.27 -0.8
13,829 - xerD MCAODC_00180 0.29 +0.2
13,877 + xerD MCAODC_00180 0.34 -0.7
13,999 + xerD MCAODC_00180 0.46 -2.8
14,000 - xerD MCAODC_00180 0.46 +0.2
14,000 - xerD MCAODC_00180 0.46 +2.2
14,038 + xerD MCAODC_00180 0.50 +0.3
14,038 + xerD MCAODC_00180 0.50 +0.7
14,038 + xerD MCAODC_00180 0.50 -0.8
14,038 + xerD MCAODC_00180 0.50 -1.4
14,038 + xerD MCAODC_00180 0.50 -0.4
14,038 + xerD MCAODC_00180 0.50 +0.2
14,039 - xerD MCAODC_00180 0.50 -0.2
14,039 - xerD MCAODC_00180 0.50 +1.3
14,042 + xerD MCAODC_00180 0.51 -1.7
14,043 - xerD MCAODC_00180 0.51 -1.8
14,043 - xerD MCAODC_00180 0.51 +0.5
14,043 - xerD MCAODC_00180 0.51 -0.3
14,082 - xerD MCAODC_00180 0.55 -1.1
14,117 + xerD MCAODC_00180 0.58 +0.9
14,167 + xerD MCAODC_00180 0.63 -1.4
14,168 - xerD MCAODC_00180 0.64 -0.4
14,168 - xerD MCAODC_00180 0.64 -0.8
14,175 + xerD MCAODC_00180 0.64 -0.1
14,359 + xerD MCAODC_00180 0.83 -0.6
14,359 + xerD MCAODC_00180 0.83 +0.7
14,360 - xerD MCAODC_00180 0.83 +3.1
14,404 - xerD MCAODC_00180 0.88 -0.3
14,420 + xerD MCAODC_00180 0.89 +1.0
14,421 - xerD MCAODC_00180 0.90 -0.3
14,421 - xerD MCAODC_00180 0.90 +0.7
14,499 - -0.6
14,511 - -0.3
14,549 + Thr_trna MCAODC_00185 0.16 -1.7
14,605 - -0.5
14,767 + -0.2
14,767 + -2.0
14,768 - -0.1
14,768 - -1.3
14,768 - -0.3
14,823 + -0.3
15,056 - proA MCAODC_00190 0.26 +0.3
15,129 + proA MCAODC_00190 0.32 -2.2
15,129 + proA MCAODC_00190 0.32 +2.7
15,180 - proA MCAODC_00190 0.36 -1.7
15,180 - proA MCAODC_00190 0.36 -0.1
15,181 + proA MCAODC_00190 0.36 +0.5
15,213 + proA MCAODC_00190 0.39 -1.1
15,219 - proA MCAODC_00190 0.39 -0.4
15,219 - proA MCAODC_00190 0.39 +0.5
15,219 - proA MCAODC_00190 0.39 +1.7
15,221 + proA MCAODC_00190 0.39 -1.8
15,221 + proA MCAODC_00190 0.39 -1.7
15,221 + proA MCAODC_00190 0.39 -0.1
15,221 + proA MCAODC_00190 0.39 +1.1
15,221 + proA MCAODC_00190 0.39 +2.3
15,222 - proA MCAODC_00190 0.39 +1.3
15,222 - proA MCAODC_00190 0.39 +2.7
15,222 - proA MCAODC_00190 0.39 -0.3
15,222 - proA MCAODC_00190 0.39 -0.2
15,222 - proA MCAODC_00190 0.39 -0.1
15,222 - proA MCAODC_00190 0.39 +1.7
15,222 - proA MCAODC_00190 0.39 +0.7
15,222 - proA MCAODC_00190 0.39 -0.4
15,222 - proA MCAODC_00190 0.39 -0.5
15,222 - proA MCAODC_00190 0.39 -1.7
15,259 - proA MCAODC_00190 0.42 +1.2
15,298 + proA MCAODC_00190 0.46 -1.6
15,302 + proA MCAODC_00190 0.46 +0.2
15,302 + proA MCAODC_00190 0.46 -0.8
15,303 - proA MCAODC_00190 0.46 -0.1
15,310 - proA MCAODC_00190 0.46 +0.3
15,403 + proA MCAODC_00190 0.54 -1.3
15,403 + proA MCAODC_00190 0.54 -1.4
15,404 - proA MCAODC_00190 0.54 -1.7
15,404 - proA MCAODC_00190 0.54 +0.1
15,404 - proA MCAODC_00190 0.54 -0.3
15,414 - proA MCAODC_00190 0.55 -0.3
15,450 - proA MCAODC_00190 0.58 -1.1

Or see this region's nucleotide sequence