Experiment: vio MOI10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_04408 and OHPLBJKB_04409 overlap by 14 nucleotides OHPLBJKB_04409 and OHPLBJKB_04410 are separated by 21 nucleotides OHPLBJKB_04410 and OHPLBJKB_04411 are separated by 14 nucleotides OHPLBJKB_04411 and OHPLBJKB_04412 are separated by 32 nucleotides OHPLBJKB_04412 and OHPLBJKB_04413 are separated by 98 nucleotides
OHPLBJKB_04408: OHPLBJKB_04408 - hypothetical protein, at 63,849 to 64,577
_04408
OHPLBJKB_04409: OHPLBJKB_04409 - hypothetical protein, at 64,564 to 65,130
_04409
OHPLBJKB_04410: OHPLBJKB_04410 - hypothetical protein, at 65,152 to 65,463
_04410
OHPLBJKB_04411: OHPLBJKB_04411 - Pilin, at 65,478 to 65,843
_04411
OHPLBJKB_04412: OHPLBJKB_04412 - Relaxosome protein TraY, at 65,876 to 66,271
_04412
OHPLBJKB_04413: OHPLBJKB_04413 - Protein TraJ, at 66,370 to 67,059
_04413
Position (kb) 65
66 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7 at 64.152 kb on + strand, within OHPLBJKB_04408 at 64.153 kb on - strand, within OHPLBJKB_04408 at 64.310 kb on + strand, within OHPLBJKB_04408 at 64.310 kb on + strand, within OHPLBJKB_04408 at 64.311 kb on - strand, within OHPLBJKB_04408 at 64.311 kb on - strand, within OHPLBJKB_04408 at 64.458 kb on - strand, within OHPLBJKB_04408 at 64.554 kb on + strand at 64.640 kb on + strand, within OHPLBJKB_04409 at 64.640 kb on + strand, within OHPLBJKB_04409 at 64.640 kb on + strand, within OHPLBJKB_04409 at 64.687 kb on - strand, within OHPLBJKB_04409 at 64.899 kb on + strand, within OHPLBJKB_04409 at 64.921 kb on + strand, within OHPLBJKB_04409 at 64.968 kb on + strand, within OHPLBJKB_04409 at 64.968 kb on + strand, within OHPLBJKB_04409 at 65.020 kb on - strand, within OHPLBJKB_04409 at 65.224 kb on - strand, within OHPLBJKB_04410 at 65.226 kb on - strand, within OHPLBJKB_04410 at 65.324 kb on + strand, within OHPLBJKB_04410 at 65.445 kb on + strand at 65.469 kb on + strand at 65.510 kb on + strand at 65.511 kb on - strand at 65.514 kb on + strand at 65.566 kb on + strand, within OHPLBJKB_04411 at 65.567 kb on - strand, within OHPLBJKB_04411 at 65.567 kb on - strand, within OHPLBJKB_04411 at 65.567 kb on - strand, within OHPLBJKB_04411 at 65.567 kb on - strand, within OHPLBJKB_04411 at 65.609 kb on + strand, within OHPLBJKB_04411 at 65.609 kb on + strand, within OHPLBJKB_04411 at 65.609 kb on + strand, within OHPLBJKB_04411 at 65.610 kb on - strand, within OHPLBJKB_04411 at 65.610 kb on - strand, within OHPLBJKB_04411 at 65.610 kb on - strand, within OHPLBJKB_04411 at 65.763 kb on - strand, within OHPLBJKB_04411 at 65.763 kb on - strand, within OHPLBJKB_04411 at 65.843 kb on + strand at 65.844 kb on - strand at 65.878 kb on - strand at 65.887 kb on + strand at 65.887 kb on + strand at 65.896 kb on + strand at 65.897 kb on - strand at 66.025 kb on + strand, within OHPLBJKB_04412 at 66.025 kb on + strand, within OHPLBJKB_04412 at 66.067 kb on - strand, within OHPLBJKB_04412 at 66.082 kb on + strand, within OHPLBJKB_04412 at 66.087 kb on + strand, within OHPLBJKB_04412 at 66.087 kb on + strand, within OHPLBJKB_04412 at 66.092 kb on - strand, within OHPLBJKB_04412 at 66.092 kb on - strand, within OHPLBJKB_04412 at 66.136 kb on + strand, within OHPLBJKB_04412 at 66.137 kb on - strand, within OHPLBJKB_04412 at 66.137 kb on - strand, within OHPLBJKB_04412 at 66.197 kb on + strand, within OHPLBJKB_04412 at 66.213 kb on - strand, within OHPLBJKB_04412 at 66.231 kb on - strand, within OHPLBJKB_04412 at 66.238 kb on + strand at 66.307 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction vio MOI10 remove 64,152 + OHPLBJKB_04408 0.42 +5.3 64,153 - OHPLBJKB_04408 0.42 +4.8 64,310 + OHPLBJKB_04408 0.63 +5.6 64,310 + OHPLBJKB_04408 0.63 +5.1 64,311 - OHPLBJKB_04408 0.63 +6.1 64,311 - OHPLBJKB_04408 0.63 +5.8 64,458 - OHPLBJKB_04408 0.84 +5.6 64,554 + +4.9 64,640 + OHPLBJKB_04409 0.13 +4.8 64,640 + OHPLBJKB_04409 0.13 +7.2 64,640 + OHPLBJKB_04409 0.13 +5.9 64,687 - OHPLBJKB_04409 0.22 +5.4 64,899 + OHPLBJKB_04409 0.59 +4.8 64,921 + OHPLBJKB_04409 0.63 +6.6 64,968 + OHPLBJKB_04409 0.71 +6.3 64,968 + OHPLBJKB_04409 0.71 +5.5 65,020 - OHPLBJKB_04409 0.80 +5.9 65,224 - OHPLBJKB_04410 0.23 +4.8 65,226 - OHPLBJKB_04410 0.24 +5.7 65,324 + OHPLBJKB_04410 0.55 +5.9 65,445 + +6.7 65,469 + +6.4 65,510 + +6.5 65,511 - +6.3 65,514 + +5.2 65,566 + OHPLBJKB_04411 0.24 +5.7 65,567 - OHPLBJKB_04411 0.24 +6.2 65,567 - OHPLBJKB_04411 0.24 +6.0 65,567 - OHPLBJKB_04411 0.24 +6.2 65,567 - OHPLBJKB_04411 0.24 +6.5 65,609 + OHPLBJKB_04411 0.36 +4.8 65,609 + OHPLBJKB_04411 0.36 +5.3 65,609 + OHPLBJKB_04411 0.36 +5.8 65,610 - OHPLBJKB_04411 0.36 +5.9 65,610 - OHPLBJKB_04411 0.36 +5.8 65,610 - OHPLBJKB_04411 0.36 +5.9 65,763 - OHPLBJKB_04411 0.78 +5.0 65,763 - OHPLBJKB_04411 0.78 +5.6 65,843 + +5.8 65,844 - +6.9 65,878 - +4.3 65,887 + +6.0 65,887 + +5.3 65,896 + +5.0 65,897 - +4.0 66,025 + OHPLBJKB_04412 0.38 +6.1 66,025 + OHPLBJKB_04412 0.38 +5.6 66,067 - OHPLBJKB_04412 0.48 -1.0 66,082 + OHPLBJKB_04412 0.52 +6.1 66,087 + OHPLBJKB_04412 0.53 +5.9 66,087 + OHPLBJKB_04412 0.53 +5.0 66,092 - OHPLBJKB_04412 0.55 +1.6 66,092 - OHPLBJKB_04412 0.55 +1.6 66,136 + OHPLBJKB_04412 0.66 +6.7 66,137 - OHPLBJKB_04412 0.66 +0.7 66,137 - OHPLBJKB_04412 0.66 +0.9 66,197 + OHPLBJKB_04412 0.81 +5.8 66,213 - OHPLBJKB_04412 0.85 +0.3 66,231 - OHPLBJKB_04412 0.90 +1.3 66,238 + +6.0 66,307 + +7.0
Or see this region's nucleotide sequence