Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt odhB and sucA are separated by 14 nucleotides sucA and sdhB are separated by 300 nucleotides
MCAODC_26140: odhB - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 1,941,413 to 1,942,630
odhB
MCAODC_26145: sucA - 2-oxoglutarate dehydrogenase E1 component, at 1,942,645 to 1,945,446
sucA
MCAODC_26155: sdhB - succinate dehydrogenase iron-sulfur subunit SdhB, at 1,945,747 to 1,946,463
sdhB
Position (kb)
1942
1943
1944
1945
1946 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1941.919 kb on - strand, within odhB at 1942.235 kb on - strand, within odhB at 1942.485 kb on - strand, within odhB at 1942.620 kb on - strand at 1942.625 kb on - strand at 1942.647 kb on - strand at 1942.705 kb on - strand at 1942.800 kb on - strand at 1942.948 kb on - strand, within sucA at 1942.948 kb on - strand, within sucA at 1942.971 kb on - strand, within sucA at 1943.532 kb on - strand, within sucA at 1943.988 kb on - strand, within sucA at 1944.027 kb on - strand, within sucA at 1944.114 kb on + strand, within sucA at 1944.223 kb on - strand, within sucA at 1944.292 kb on - strand, within sucA at 1944.292 kb on - strand, within sucA at 1944.493 kb on - strand, within sucA at 1944.730 kb on - strand, within sucA at 1944.836 kb on - strand, within sucA at 1945.543 kb on - strand at 1945.543 kb on - strand at 1945.547 kb on - strand at 1945.581 kb on - strand at 1945.644 kb on - strand at 1945.741 kb on + strand at 1945.748 kb on + strand at 1945.756 kb on - strand at 1945.756 kb on - strand at 1945.779 kb on + strand at 1945.819 kb on + strand, within sdhB at 1945.820 kb on - strand, within sdhB at 1945.820 kb on - strand, within sdhB at 1945.842 kb on + strand, within sdhB at 1945.842 kb on + strand, within sdhB at 1945.843 kb on - strand, within sdhB at 1945.843 kb on - strand, within sdhB at 1945.843 kb on - strand, within sdhB at 1946.016 kb on + strand, within sdhB at 1946.017 kb on - strand, within sdhB at 1946.017 kb on - strand, within sdhB at 1946.017 kb on - strand, within sdhB at 1946.095 kb on - strand, within sdhB at 1946.106 kb on - strand, within sdhB at 1946.239 kb on - strand, within sdhB at 1946.396 kb on + strand at 1946.397 kb on - strand at 1946.397 kb on - strand at 1946.435 kb on + strand at 1946.436 kb on - strand at 1946.441 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 1,941,919 - odhB MCAODC_26140 0.42 -2.9 1,942,235 - odhB MCAODC_26140 0.67 -0.3 1,942,485 - odhB MCAODC_26140 0.88 +2.5 1,942,620 - -0.1 1,942,625 - +1.7 1,942,647 - -1.6 1,942,705 - +1.2 1,942,800 - +1.7 1,942,948 - sucA MCAODC_26145 0.11 +1.7 1,942,948 - sucA MCAODC_26145 0.11 +2.2 1,942,971 - sucA MCAODC_26145 0.12 +0.7 1,943,532 - sucA MCAODC_26145 0.32 -0.3 1,943,988 - sucA MCAODC_26145 0.48 -1.4 1,944,027 - sucA MCAODC_26145 0.49 +2.3 1,944,114 + sucA MCAODC_26145 0.52 -0.3 1,944,223 - sucA MCAODC_26145 0.56 +1.7 1,944,292 - sucA MCAODC_26145 0.59 +1.7 1,944,292 - sucA MCAODC_26145 0.59 +1.7 1,944,493 - sucA MCAODC_26145 0.66 +1.7 1,944,730 - sucA MCAODC_26145 0.74 +0.7 1,944,836 - sucA MCAODC_26145 0.78 +1.0 1,945,543 - +2.7 1,945,543 - -0.3 1,945,547 - +0.6 1,945,581 - -0.6 1,945,644 - +0.2 1,945,741 + -0.1 1,945,748 + -0.3 1,945,756 - +0.3 1,945,756 - +0.7 1,945,779 + +0.9 1,945,819 + sdhB MCAODC_26155 0.10 +1.7 1,945,820 - sdhB MCAODC_26155 0.10 +1.7 1,945,820 - sdhB MCAODC_26155 0.10 +3.1 1,945,842 + sdhB MCAODC_26155 0.13 +0.7 1,945,842 + sdhB MCAODC_26155 0.13 +0.7 1,945,843 - sdhB MCAODC_26155 0.13 +2.3 1,945,843 - sdhB MCAODC_26155 0.13 +0.7 1,945,843 - sdhB MCAODC_26155 0.13 +0.2 1,946,016 + sdhB MCAODC_26155 0.38 -0.4 1,946,017 - sdhB MCAODC_26155 0.38 +1.7 1,946,017 - sdhB MCAODC_26155 0.38 +1.0 1,946,017 - sdhB MCAODC_26155 0.38 -0.6 1,946,095 - sdhB MCAODC_26155 0.49 -0.6 1,946,106 - sdhB MCAODC_26155 0.50 +0.7 1,946,239 - sdhB MCAODC_26155 0.69 +1.7 1,946,396 + -1.6 1,946,397 - +1.4 1,946,397 - +0.2 1,946,435 + -0.5 1,946,436 - -1.4 1,946,441 + +0.4
Or see this region's nucleotide sequence