Strain Fitness in Escherichia coli ECRC101 around MCAODC_21815

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntprfA and hemA are separated by 41 nucleotideshemA and lolB are separated by 213 nucleotideslolB and ispE overlap by 1 nucleotides MCAODC_21810: prfA - peptide chain release factor 1, at 1,143,878 to 1,144,960 prfA MCAODC_21815: hemA - glutamyl-tRNA reductase, at 1,145,002 to 1,146,258 hemA MCAODC_21820: lolB - lipoprotein insertase outer membrane protein LolB, at 1,146,472 to 1,147,095 lolB MCAODC_21825: ispE - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 1,147,095 to 1,147,946 ispE Position (kb) 1145 1146 1147Strain fitness (log2 ratio) -1 0 1at 1144.998 kb on - strandat 1144.998 kb on - strandat 1145.004 kb on - strandat 1146.287 kb on - strandat 1146.293 kb on - strandat 1146.354 kb on - strandat 1146.384 kb on + strandat 1146.385 kb on - strandat 1146.436 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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1,144,998 - -0.1
1,144,998 - +0.8
1,145,004 - -0.9
1,146,287 - -0.9
1,146,293 - +1.7
1,146,354 - -0.0
1,146,384 + -0.3
1,146,385 - +0.7
1,146,436 + +0.4

Or see this region's nucleotide sequence