Strain Fitness in Escherichia coli ECRC101 around MCAODC_20865

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_20855 and MCAODC_20860 overlap by 4 nucleotidesMCAODC_20860 and MCAODC_20865 are separated by 66 nucleotidesMCAODC_20865 and MCAODC_20870 overlap by 8 nucleotidesMCAODC_20870 and lysC overlap by 38 nucleotideslysC and lysB overlap by 14 nucleotideslysB and lysA overlap by 13 nucleotides MCAODC_20855: MCAODC_20855 - baseplate assembly protein, at 982,194 to 982,541 _20855 MCAODC_20860: MCAODC_20860 - Spike protein, at 982,538 to 983,173 _20860 MCAODC_20865: MCAODC_20865 - phage virion morphogenesis protein, at 983,240 to 983,692 _20865 MCAODC_20870: MCAODC_20870 - Tail completion protein R, at 983,685 to 984,152 _20870 MCAODC_20875: lysC - Rz1-like lysis system protein LysC, at 984,115 to 984,273 lysC MCAODC_20880: lysB - Rz-like lysis system protein LysB, at 984,260 to 984,685 lysB MCAODC_20885: lysA - Putative antiholin, at 984,673 to 985,098 lysA Position (kb) 983 984Strain fitness (log2 ratio) -1 0 1 2at 982.340 kb on - strand, within MCAODC_20855at 982.395 kb on + strand, within MCAODC_20855at 982.449 kb on - strand, within MCAODC_20855at 982.449 kb on - strand, within MCAODC_20855at 982.449 kb on - strand, within MCAODC_20855at 982.478 kb on + strand, within MCAODC_20855at 982.478 kb on + strand, within MCAODC_20855at 982.595 kb on + strandat 982.596 kb on - strandat 982.596 kb on - strandat 982.600 kb on + strandat 982.801 kb on + strand, within MCAODC_20860at 982.839 kb on + strand, within MCAODC_20860at 982.917 kb on + strand, within MCAODC_20860at 983.241 kb on + strandat 983.242 kb on - strandat 983.242 kb on - strandat 983.263 kb on + strandat 983.322 kb on + strand, within MCAODC_20865at 983.462 kb on - strand, within MCAODC_20865at 983.465 kb on - strand, within MCAODC_20865at 983.720 kb on + strandat 983.721 kb on - strandat 983.721 kb on - strandat 983.771 kb on + strand, within MCAODC_20870at 983.844 kb on - strand, within MCAODC_20870at 984.010 kb on + strand, within MCAODC_20870at 984.054 kb on - strand, within MCAODC_20870at 984.054 kb on - strand, within MCAODC_20870at 984.072 kb on + strand, within MCAODC_20870at 984.084 kb on - strand, within MCAODC_20870at 984.174 kb on + strand, within lysCat 984.174 kb on + strand, within lysCat 984.174 kb on + strand, within lysCat 984.175 kb on - strand, within lysCat 984.184 kb on + strand, within lysCat 984.239 kb on - strand, within lysCat 984.410 kb on + strand, within lysB

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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982,340 - MCAODC_20855 0.42 +0.1
982,395 + MCAODC_20855 0.58 -0.8
982,449 - MCAODC_20855 0.73 +1.1
982,449 - MCAODC_20855 0.73 +0.4
982,449 - MCAODC_20855 0.73 -0.6
982,478 + MCAODC_20855 0.82 +0.1
982,478 + MCAODC_20855 0.82 -0.3
982,595 + -0.6
982,596 - +0.5
982,596 - -0.1
982,600 + -0.4
982,801 + MCAODC_20860 0.41 +0.4
982,839 + MCAODC_20860 0.47 -0.4
982,917 + MCAODC_20860 0.60 +1.1
983,241 + -0.4
983,242 - -1.5
983,242 - +0.9
983,263 + +1.7
983,322 + MCAODC_20865 0.18 -1.2
983,462 - MCAODC_20865 0.49 +1.7
983,465 - MCAODC_20865 0.50 -0.5
983,720 + -0.1
983,721 - -0.6
983,721 - +1.0
983,771 + MCAODC_20870 0.18 -0.2
983,844 - MCAODC_20870 0.34 -0.3
984,010 + MCAODC_20870 0.69 +0.7
984,054 - MCAODC_20870 0.79 +0.1
984,054 - MCAODC_20870 0.79 +1.5
984,072 + MCAODC_20870 0.83 +2.7
984,084 - MCAODC_20870 0.85 +0.2
984,174 + lysC MCAODC_20875 0.37 +0.4
984,174 + lysC MCAODC_20875 0.37 -0.4
984,174 + lysC MCAODC_20875 0.37 +0.3
984,175 - lysC MCAODC_20875 0.38 +0.4
984,184 + lysC MCAODC_20875 0.43 +0.4
984,239 - lysC MCAODC_20875 0.78 -0.3
984,410 + lysB MCAODC_20880 0.35 -0.1

Or see this region's nucleotide sequence