Strain Fitness in Escherichia coli ECRC101 around MCAODC_20325

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_20320 and MCAODC_20325 are separated by 81 nucleotidesMCAODC_20325 and yeeS are separated by 14 nucleotidesyeeS and yeeT are separated by 62 nucleotidesyeeT and cbtA are separated by 73 nucleotides MCAODC_20320: MCAODC_20320 - DUF932 domain-containing protein, at 875,278 to 876,099 _20320 MCAODC_20325: MCAODC_20325 - restriction endonuclease, at 876,181 to 876,660 _20325 MCAODC_20330: yeeS - UPF0758 protein YeeS, at 876,675 to 877,151 yeeS MCAODC_20335: yeeT - Uncharacterized protein YeeT, at 877,214 to 877,435 yeeT MCAODC_20340: cbtA - type IV toxin-antitoxin system toxin CbtA, at 877,509 to 877,877 cbtA Position (kb) 876 877Strain fitness (log2 ratio) -1 0 1at 875.303 kb on - strandat 875.318 kb on + strandat 875.363 kb on + strand, within MCAODC_20320at 875.364 kb on - strand, within MCAODC_20320at 875.416 kb on + strand, within MCAODC_20320at 875.416 kb on + strand, within MCAODC_20320at 875.430 kb on + strand, within MCAODC_20320at 875.555 kb on + strand, within MCAODC_20320at 875.615 kb on - strand, within MCAODC_20320at 875.615 kb on - strand, within MCAODC_20320at 875.633 kb on - strand, within MCAODC_20320at 875.959 kb on - strand, within MCAODC_20320at 876.222 kb on + strandat 876.468 kb on - strand, within MCAODC_20325at 876.544 kb on + strand, within MCAODC_20325at 876.544 kb on + strand, within MCAODC_20325at 876.574 kb on + strand, within MCAODC_20325at 876.575 kb on - strand, within MCAODC_20325at 876.592 kb on + strand, within MCAODC_20325at 877.186 kb on + strandat 877.404 kb on - strand, within yeeTat 877.404 kb on - strand, within yeeTat 877.469 kb on - strandat 877.469 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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875,303 - +1.6
875,318 + -0.4
875,363 + MCAODC_20320 0.10 -0.8
875,364 - MCAODC_20320 0.10 -1.7
875,416 + MCAODC_20320 0.17 -0.3
875,416 + MCAODC_20320 0.17 +0.8
875,430 + MCAODC_20320 0.18 -0.5
875,555 + MCAODC_20320 0.34 +0.4
875,615 - MCAODC_20320 0.41 +0.2
875,615 - MCAODC_20320 0.41 -0.9
875,633 - MCAODC_20320 0.43 -0.7
875,959 - MCAODC_20320 0.83 +1.1
876,222 + +1.2
876,468 - MCAODC_20325 0.60 +1.0
876,544 + MCAODC_20325 0.76 -0.8
876,544 + MCAODC_20325 0.76 -0.3
876,574 + MCAODC_20325 0.82 -1.5
876,575 - MCAODC_20325 0.82 -0.2
876,592 + MCAODC_20325 0.86 +1.7
877,186 + -0.7
877,404 - yeeT MCAODC_20335 0.86 -1.2
877,404 - yeeT MCAODC_20335 0.86 +1.2
877,469 - +0.7
877,469 - +0.5

Or see this region's nucleotide sequence