Strain Fitness in Escherichia coli ECRC101 around MCAODC_16115

Experiment: EV240

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuspF and MCAODC_16110 are separated by 140 nucleotidesMCAODC_16110 and MCAODC_16115 overlap by 26 nucleotidesMCAODC_16115 and nifJ are separated by 365 nucleotides MCAODC_16105: uspF - universal stress protein UspF, at 110,271 to 110,705 uspF MCAODC_16110: MCAODC_16110 - hypothetical protein, at 110,846 to 111,613 _16110 MCAODC_16115: MCAODC_16115 - Putative outer membrane protein, at 111,588 to 111,959 _16115 MCAODC_16125: nifJ - pyruvate:ferredoxin (flavodoxin) oxidoreductase, at 112,325 to 115,849 nifJ Position (kb) 111 112Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 110.705 kb on + strandat 110.788 kb on + strandat 110.788 kb on + strandat 110.867 kb on + strandat 110.878 kb on + strandat 110.878 kb on + strandat 110.907 kb on + strandat 110.952 kb on + strand, within MCAODC_16110at 110.979 kb on + strand, within MCAODC_16110at 111.190 kb on + strand, within MCAODC_16110at 111.191 kb on - strand, within MCAODC_16110at 111.223 kb on - strand, within MCAODC_16110at 111.227 kb on - strand, within MCAODC_16110at 111.289 kb on + strand, within MCAODC_16110at 111.363 kb on + strand, within MCAODC_16110at 111.440 kb on - strand, within MCAODC_16110at 111.458 kb on - strand, within MCAODC_16110at 111.458 kb on - strand, within MCAODC_16110at 111.569 kb on - strandat 111.614 kb on - strandat 111.618 kb on - strandat 112.068 kb on - strandat 112.199 kb on + strandat 112.563 kb on - strandat 112.647 kb on + strandat 112.648 kb on - strandat 112.755 kb on + strand, within nifJat 112.755 kb on + strand, within nifJat 112.756 kb on - strand, within nifJat 112.756 kb on - strand, within nifJat 112.793 kb on - strand, within nifJat 112.951 kb on - strand, within nifJ

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Per-strain Table

Position Strand Gene LocusTag Fraction EV240
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110,705 + -1.0
110,788 + +0.5
110,788 + +1.1
110,867 + -0.1
110,878 + +2.5
110,878 + -1.9
110,907 + -3.4
110,952 + MCAODC_16110 0.14 +1.5
110,979 + MCAODC_16110 0.17 +1.5
111,190 + MCAODC_16110 0.45 -2.7
111,191 - MCAODC_16110 0.45 -1.9
111,223 - MCAODC_16110 0.49 +0.1
111,227 - MCAODC_16110 0.50 -0.8
111,289 + MCAODC_16110 0.58 -0.1
111,363 + MCAODC_16110 0.67 -2.7
111,440 - MCAODC_16110 0.77 +1.5
111,458 - MCAODC_16110 0.80 -1.3
111,458 - MCAODC_16110 0.80 -0.5
111,569 - +0.3
111,614 - +0.6
111,618 - -1.3
112,068 - +1.1
112,199 + -0.1
112,563 - -1.1
112,647 + -0.1
112,648 - -1.3
112,755 + nifJ MCAODC_16125 0.12 -0.9
112,755 + nifJ MCAODC_16125 0.12 +0.3
112,756 - nifJ MCAODC_16125 0.12 +0.5
112,756 - nifJ MCAODC_16125 0.12 +0.4
112,793 - nifJ MCAODC_16125 0.13 +0.5
112,951 - nifJ MCAODC_16125 0.18 +0.9

Or see this region's nucleotide sequence