Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pspF and pspA are separated by 166 nucleotides pspA and pspB are separated by 53 nucleotides pspB and pspC overlap by 1 nucleotides pspC and pspD are separated by 8 nucleotides pspD and pspE are separated by 74 nucleotides
MCAODC_15855: pspF - phage shock protein operon transcriptional activator, at 64,742 to 65,719
pspF
MCAODC_15860: pspA - phage shock protein PspA, at 65,886 to 66,554
pspA
MCAODC_15865: pspB - envelope stress response membrane protein PspB, at 66,608 to 66,832
pspB
MCAODC_15870: pspC - envelope stress response membrane protein PspC, at 66,832 to 67,191
pspC
MCAODC_15875: pspD - phage shock protein PspD, at 67,200 to 67,421
pspD
MCAODC_15880: pspE - thiosulfate sulfurtransferase PspE, at 67,496 to 67,810
pspE
Position (kb) 65
66
67 Strain fitness (log2 ratio) -2
-1
0
1
2 at 64.967 kb on - strand, within pspF at 65.161 kb on + strand, within pspF at 65.162 kb on - strand, within pspF at 65.162 kb on - strand, within pspF at 65.344 kb on - strand, within pspF at 65.344 kb on - strand, within pspF at 65.409 kb on - strand, within pspF at 65.409 kb on - strand, within pspF at 65.539 kb on + strand, within pspF at 65.568 kb on + strand, within pspF at 65.701 kb on + strand at 65.885 kb on + strand at 65.891 kb on + strand at 65.941 kb on + strand at 65.999 kb on + strand, within pspA at 65.999 kb on + strand, within pspA at 65.999 kb on + strand, within pspA at 65.999 kb on + strand, within pspA at 66.000 kb on - strand, within pspA at 66.013 kb on + strand, within pspA at 66.065 kb on + strand, within pspA at 66.407 kb on + strand, within pspA at 66.555 kb on + strand at 66.555 kb on + strand at 66.556 kb on - strand at 66.596 kb on + strand at 66.608 kb on - strand at 66.793 kb on + strand, within pspB at 66.843 kb on + strand at 66.916 kb on + strand, within pspC at 67.000 kb on + strand, within pspC at 67.000 kb on + strand, within pspC at 67.001 kb on - strand, within pspC at 67.141 kb on + strand, within pspC at 67.141 kb on + strand, within pspC at 67.142 kb on - strand, within pspC at 67.379 kb on - strand, within pspD at 67.456 kb on + strand at 67.459 kb on - strand at 67.481 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 64,967 - pspF MCAODC_15855 0.23 -0.5 65,161 + pspF MCAODC_15855 0.43 +0.5 65,162 - pspF MCAODC_15855 0.43 +1.5 65,162 - pspF MCAODC_15855 0.43 -1.7 65,344 - pspF MCAODC_15855 0.62 +0.9 65,344 - pspF MCAODC_15855 0.62 -0.1 65,409 - pspF MCAODC_15855 0.68 +0.4 65,409 - pspF MCAODC_15855 0.68 +0.1 65,539 + pspF MCAODC_15855 0.81 -0.5 65,568 + pspF MCAODC_15855 0.84 +0.4 65,701 + +0.5 65,885 + +1.2 65,891 + -1.5 65,941 + -1.5 65,999 + pspA MCAODC_15860 0.17 +0.3 65,999 + pspA MCAODC_15860 0.17 +0.5 65,999 + pspA MCAODC_15860 0.17 +0.6 65,999 + pspA MCAODC_15860 0.17 +2.5 66,000 - pspA MCAODC_15860 0.17 -2.1 66,013 + pspA MCAODC_15860 0.19 -0.8 66,065 + pspA MCAODC_15860 0.27 -0.5 66,407 + pspA MCAODC_15860 0.78 +0.7 66,555 + -0.8 66,555 + -0.5 66,556 - +0.5 66,596 + -1.3 66,608 - -0.1 66,793 + pspB MCAODC_15865 0.82 -0.7 66,843 + -2.4 66,916 + pspC MCAODC_15870 0.23 -1.8 67,000 + pspC MCAODC_15870 0.47 -0.3 67,000 + pspC MCAODC_15870 0.47 -0.1 67,001 - pspC MCAODC_15870 0.47 +0.1 67,141 + pspC MCAODC_15870 0.86 +1.5 67,141 + pspC MCAODC_15870 0.86 -0.2 67,142 - pspC MCAODC_15870 0.86 +0.9 67,379 - pspD MCAODC_15875 0.81 -0.2 67,456 + +2.2 67,459 - -0.7 67,481 + -2.7
Or see this region's nucleotide sequence