Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpE and trpL are separated by 91 nucleotides trpL and yciV are separated by 137 nucleotides yciV and yciO overlap by 4 nucleotides yciO and yciQ are separated by 27 nucleotides
MCAODC_15610: trpE - anthranilate synthase component I, at 12,391 to 13,953
trpE
MCAODC_15615: trpL - trp operon leader peptide, at 14,045 to 14,089
trpL
MCAODC_15620: yciV - 5'-3' exoribonuclease, at 14,227 to 15,108
yciV
MCAODC_15625: yciO - L-threonylcarbamoyladenylate synthase, at 15,105 to 15,725
yciO
MCAODC_15630: yciQ - Uncharacterized protein YciQ, at 15,753 to 17,336
yciQ
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 13.449 kb on + strand, within trpE at 13.500 kb on - strand, within trpE at 13.600 kb on + strand, within trpE at 13.600 kb on + strand, within trpE at 13.664 kb on - strand, within trpE at 13.790 kb on - strand, within trpE at 13.807 kb on + strand at 13.808 kb on - strand at 13.958 kb on - strand at 14.243 kb on - strand at 14.271 kb on + strand at 14.499 kb on + strand, within yciV at 14.499 kb on + strand, within yciV at 14.592 kb on + strand, within yciV at 14.593 kb on - strand, within yciV at 14.593 kb on - strand, within yciV at 14.593 kb on - strand, within yciV at 14.593 kb on - strand, within yciV at 14.594 kb on + strand, within yciV at 14.595 kb on - strand, within yciV at 14.765 kb on + strand, within yciV at 14.775 kb on + strand, within yciV at 14.775 kb on + strand, within yciV at 14.775 kb on + strand, within yciV at 14.813 kb on + strand, within yciV at 14.934 kb on + strand, within yciV at 14.969 kb on + strand, within yciV at 15.027 kb on + strand at 15.068 kb on + strand at 15.104 kb on + strand at 15.104 kb on + strand at 15.122 kb on + strand at 15.122 kb on + strand at 15.122 kb on + strand at 15.123 kb on - strand at 15.152 kb on - strand at 15.152 kb on - strand at 15.180 kb on - strand, within yciO at 15.198 kb on + strand, within yciO at 15.336 kb on + strand, within yciO at 15.393 kb on + strand, within yciO at 15.394 kb on - strand, within yciO at 15.395 kb on + strand, within yciO at 15.464 kb on + strand, within yciO at 15.617 kb on + strand, within yciO at 15.700 kb on + strand at 15.721 kb on + strand at 15.721 kb on + strand at 15.752 kb on + strand at 15.787 kb on + strand at 15.787 kb on + strand at 15.811 kb on + strand at 15.843 kb on + strand at 15.947 kb on + strand, within yciQ at 15.947 kb on + strand, within yciQ at 15.979 kb on + strand, within yciQ at 15.979 kb on + strand, within yciQ at 15.980 kb on - strand, within yciQ at 16.017 kb on + strand, within yciQ at 16.022 kb on + strand, within yciQ at 16.022 kb on + strand, within yciQ at 16.022 kb on + strand, within yciQ at 16.023 kb on - strand, within yciQ
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 13,449 + trpE MCAODC_15610 0.68 -0.5 13,500 - trpE MCAODC_15610 0.71 +0.5 13,600 + trpE MCAODC_15610 0.77 -0.8 13,600 + trpE MCAODC_15610 0.77 -1.5 13,664 - trpE MCAODC_15610 0.81 +3.1 13,790 - trpE MCAODC_15610 0.90 +0.2 13,807 + +0.5 13,808 - +0.9 13,958 - -2.2 14,243 - -1.4 14,271 + -1.4 14,499 + yciV MCAODC_15620 0.31 +0.5 14,499 + yciV MCAODC_15620 0.31 -1.3 14,592 + yciV MCAODC_15620 0.41 -0.5 14,593 - yciV MCAODC_15620 0.41 +0.5 14,593 - yciV MCAODC_15620 0.41 +4.1 14,593 - yciV MCAODC_15620 0.41 +0.3 14,593 - yciV MCAODC_15620 0.41 -0.1 14,594 + yciV MCAODC_15620 0.42 +2.5 14,595 - yciV MCAODC_15620 0.42 -0.3 14,765 + yciV MCAODC_15620 0.61 +0.1 14,775 + yciV MCAODC_15620 0.62 +1.0 14,775 + yciV MCAODC_15620 0.62 -0.5 14,775 + yciV MCAODC_15620 0.62 +0.7 14,813 + yciV MCAODC_15620 0.66 -1.1 14,934 + yciV MCAODC_15620 0.80 -1.2 14,969 + yciV MCAODC_15620 0.84 +0.3 15,027 + +0.5 15,068 + -0.9 15,104 + +0.4 15,104 + -1.8 15,122 + -0.5 15,122 + -1.7 15,122 + -0.1 15,123 - -1.1 15,152 - +1.5 15,152 - -1.7 15,180 - yciO MCAODC_15625 0.12 -1.0 15,198 + yciO MCAODC_15625 0.15 -0.1 15,336 + yciO MCAODC_15625 0.37 -0.1 15,393 + yciO MCAODC_15625 0.46 -1.3 15,394 - yciO MCAODC_15625 0.47 -0.7 15,395 + yciO MCAODC_15625 0.47 -1.4 15,464 + yciO MCAODC_15625 0.58 +1.1 15,617 + yciO MCAODC_15625 0.82 -0.1 15,700 + -0.5 15,721 + -1.3 15,721 + -1.2 15,752 + -0.1 15,787 + -0.4 15,787 + -1.7 15,811 + -2.5 15,843 + -0.3 15,947 + yciQ MCAODC_15630 0.12 -0.8 15,947 + yciQ MCAODC_15630 0.12 +2.3 15,979 + yciQ MCAODC_15630 0.14 -0.1 15,979 + yciQ MCAODC_15630 0.14 -0.8 15,980 - yciQ MCAODC_15630 0.14 -0.7 16,017 + yciQ MCAODC_15630 0.17 +1.8 16,022 + yciQ MCAODC_15630 0.17 -0.2 16,022 + yciQ MCAODC_15630 0.17 -2.5 16,022 + yciQ MCAODC_15630 0.17 -0.5 16,023 - yciQ MCAODC_15630 0.17 +1.5
Or see this region's nucleotide sequence