Experiment: EV240
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaA and yaaX are separated by 152 nucleotides yaaX and thrC are separated by 213 nucleotides thrC and thrB are separated by 0 nucleotides thrB and thrA are separated by 1 nucleotides
MCAODC_01605: yaaA - peroxide stress protein YaaA, at 310,714 to 311,490
yaaA
MCAODC_01610: yaaX - Uncharacterized protein YaaX, at 311,643 to 311,939
yaaX
MCAODC_01615: thrC - threonine synthase, at 312,153 to 313,439
thrC
MCAODC_01620: thrB - homoserine kinase, at 313,440 to 314,372
thrB
MCAODC_01625: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 314,374 to 316,836
thrA
Position (kb) 312
313
314 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 311.160 kb on + strand, within yaaA at 311.236 kb on + strand, within yaaA at 311.236 kb on + strand, within yaaA at 311.236 kb on + strand, within yaaA at 311.242 kb on - strand, within yaaA at 311.321 kb on + strand, within yaaA at 311.321 kb on + strand, within yaaA at 311.321 kb on + strand, within yaaA at 311.332 kb on + strand, within yaaA at 311.332 kb on + strand, within yaaA at 311.332 kb on + strand, within yaaA at 311.332 kb on + strand, within yaaA at 311.333 kb on - strand, within yaaA at 311.412 kb on + strand, within yaaA at 311.412 kb on + strand, within yaaA at 311.425 kb on + strand at 311.426 kb on - strand at 311.496 kb on + strand at 311.497 kb on - strand at 311.497 kb on - strand at 311.556 kb on + strand at 311.557 kb on - strand at 311.557 kb on - strand at 311.557 kb on - strand at 311.558 kb on + strand at 311.699 kb on + strand, within yaaX at 311.699 kb on + strand, within yaaX at 311.758 kb on + strand, within yaaX at 311.758 kb on + strand, within yaaX at 311.758 kb on + strand, within yaaX at 311.759 kb on - strand, within yaaX at 311.759 kb on - strand, within yaaX at 311.806 kb on + strand, within yaaX at 311.810 kb on - strand, within yaaX at 311.810 kb on - strand, within yaaX at 311.839 kb on - strand, within yaaX at 311.839 kb on - strand, within yaaX at 311.861 kb on + strand, within yaaX at 311.861 kb on + strand, within yaaX at 311.862 kb on - strand, within yaaX at 311.921 kb on + strand at 311.921 kb on + strand at 311.922 kb on - strand at 311.953 kb on + strand at 311.954 kb on - strand at 311.954 kb on - strand at 311.954 kb on - strand at 311.954 kb on - strand at 312.114 kb on + strand at 312.115 kb on - strand at 312.147 kb on + strand at 312.148 kb on - strand at 312.148 kb on - strand at 312.148 kb on - strand at 312.154 kb on + strand at 312.154 kb on + strand at 312.155 kb on - strand at 312.155 kb on - strand at 312.206 kb on + strand at 312.222 kb on + strand at 312.294 kb on - strand, within thrC at 312.397 kb on + strand, within thrC at 312.457 kb on + strand, within thrC at 312.632 kb on + strand, within thrC at 312.632 kb on + strand, within thrC at 312.632 kb on + strand, within thrC at 312.798 kb on - strand, within thrC at 312.806 kb on + strand, within thrC at 312.807 kb on - strand, within thrC at 312.926 kb on + strand, within thrC at 312.927 kb on - strand, within thrC at 312.927 kb on - strand, within thrC at 313.003 kb on + strand, within thrC at 313.004 kb on - strand, within thrC at 313.079 kb on + strand, within thrC at 313.121 kb on + strand, within thrC at 313.121 kb on + strand, within thrC at 313.121 kb on + strand, within thrC at 313.151 kb on - strand, within thrC at 313.253 kb on + strand, within thrC at 313.421 kb on + strand at 313.422 kb on - strand at 313.422 kb on - strand at 313.422 kb on - strand at 313.429 kb on + strand at 313.441 kb on + strand at 313.452 kb on + strand at 313.484 kb on + strand at 313.484 kb on + strand at 313.484 kb on + strand at 313.513 kb on + strand at 313.513 kb on + strand at 313.513 kb on + strand at 313.514 kb on - strand at 313.520 kb on + strand at 313.599 kb on - strand, within thrB at 313.685 kb on + strand, within thrB at 313.729 kb on + strand, within thrB at 313.730 kb on - strand, within thrB at 313.832 kb on + strand, within thrB at 313.922 kb on + strand, within thrB at 313.922 kb on + strand, within thrB at 313.923 kb on - strand, within thrB at 314.018 kb on + strand, within thrB at 314.085 kb on + strand, within thrB at 314.176 kb on + strand, within thrB at 314.268 kb on + strand, within thrB at 314.268 kb on + strand, within thrB at 314.268 kb on + strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.269 kb on - strand, within thrB at 314.336 kb on + strand at 314.337 kb on - strand at 314.384 kb on - strand at 314.384 kb on - strand at 314.401 kb on - strand at 314.401 kb on - strand at 314.401 kb on - strand at 314.404 kb on + strand at 314.404 kb on + strand at 314.405 kb on - strand at 314.405 kb on - strand at 314.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV240 remove 311,160 + yaaA MCAODC_01605 0.57 -0.1 311,236 + yaaA MCAODC_01605 0.67 -1.0 311,236 + yaaA MCAODC_01605 0.67 +0.2 311,236 + yaaA MCAODC_01605 0.67 +1.8 311,242 - yaaA MCAODC_01605 0.68 +0.3 311,321 + yaaA MCAODC_01605 0.78 +0.1 311,321 + yaaA MCAODC_01605 0.78 +0.5 311,321 + yaaA MCAODC_01605 0.78 +0.8 311,332 + yaaA MCAODC_01605 0.80 -0.0 311,332 + yaaA MCAODC_01605 0.80 -0.6 311,332 + yaaA MCAODC_01605 0.80 -0.2 311,332 + yaaA MCAODC_01605 0.80 -0.2 311,333 - yaaA MCAODC_01605 0.80 -0.2 311,412 + yaaA MCAODC_01605 0.90 +1.0 311,412 + yaaA MCAODC_01605 0.90 +1.2 311,425 + -0.5 311,426 - -0.5 311,496 + -0.3 311,497 - -1.0 311,497 - -1.9 311,556 + +0.1 311,557 - +0.1 311,557 - +0.3 311,557 - +0.5 311,558 + +0.6 311,699 + yaaX MCAODC_01610 0.19 +0.1 311,699 + yaaX MCAODC_01610 0.19 -0.1 311,758 + yaaX MCAODC_01610 0.39 +0.4 311,758 + yaaX MCAODC_01610 0.39 +1.0 311,758 + yaaX MCAODC_01610 0.39 -0.7 311,759 - yaaX MCAODC_01610 0.39 -1.7 311,759 - yaaX MCAODC_01610 0.39 -1.8 311,806 + yaaX MCAODC_01610 0.55 +0.4 311,810 - yaaX MCAODC_01610 0.56 +4.1 311,810 - yaaX MCAODC_01610 0.56 +0.0 311,839 - yaaX MCAODC_01610 0.66 +2.4 311,839 - yaaX MCAODC_01610 0.66 -0.0 311,861 + yaaX MCAODC_01610 0.73 -0.9 311,861 + yaaX MCAODC_01610 0.73 +0.3 311,862 - yaaX MCAODC_01610 0.74 -0.5 311,921 + +1.0 311,921 + -0.1 311,922 - -0.1 311,953 + -0.2 311,954 - -0.2 311,954 - +0.1 311,954 - -1.2 311,954 - +0.3 312,114 + +0.4 312,115 - -0.9 312,147 + +1.3 312,148 - +1.8 312,148 - +0.6 312,148 - -0.4 312,154 + -0.6 312,154 + +0.5 312,155 - -0.5 312,155 - -0.5 312,206 + -1.0 312,222 + +0.3 312,294 - thrC MCAODC_01615 0.11 +1.5 312,397 + thrC MCAODC_01615 0.19 -1.6 312,457 + thrC MCAODC_01615 0.24 -0.1 312,632 + thrC MCAODC_01615 0.37 +0.4 312,632 + thrC MCAODC_01615 0.37 +0.8 312,632 + thrC MCAODC_01615 0.37 -1.1 312,798 - thrC MCAODC_01615 0.50 -0.2 312,806 + thrC MCAODC_01615 0.51 +0.8 312,807 - thrC MCAODC_01615 0.51 +2.8 312,926 + thrC MCAODC_01615 0.60 +2.2 312,927 - thrC MCAODC_01615 0.60 +1.0 312,927 - thrC MCAODC_01615 0.60 +0.6 313,003 + thrC MCAODC_01615 0.66 -0.1 313,004 - thrC MCAODC_01615 0.66 -0.4 313,079 + thrC MCAODC_01615 0.72 -0.8 313,121 + thrC MCAODC_01615 0.75 -1.2 313,121 + thrC MCAODC_01615 0.75 -0.2 313,121 + thrC MCAODC_01615 0.75 -0.8 313,151 - thrC MCAODC_01615 0.78 -0.9 313,253 + thrC MCAODC_01615 0.85 -1.6 313,421 + +0.8 313,422 - -1.7 313,422 - -1.0 313,422 - -0.5 313,429 + -0.2 313,441 + -0.1 313,452 + -2.0 313,484 + +0.9 313,484 + +0.5 313,484 + -1.1 313,513 + -1.6 313,513 + +0.8 313,513 + +2.8 313,514 - +0.5 313,520 + -0.3 313,599 - thrB MCAODC_01620 0.17 -1.3 313,685 + thrB MCAODC_01620 0.26 +0.3 313,729 + thrB MCAODC_01620 0.31 +1.3 313,730 - thrB MCAODC_01620 0.31 -0.6 313,832 + thrB MCAODC_01620 0.42 +2.8 313,922 + thrB MCAODC_01620 0.52 +1.2 313,922 + thrB MCAODC_01620 0.52 +1.0 313,923 - thrB MCAODC_01620 0.52 -1.4 314,018 + thrB MCAODC_01620 0.62 +0.5 314,085 + thrB MCAODC_01620 0.69 -0.6 314,176 + thrB MCAODC_01620 0.79 -0.5 314,268 + thrB MCAODC_01620 0.89 +0.6 314,268 + thrB MCAODC_01620 0.89 -0.4 314,268 + thrB MCAODC_01620 0.89 +0.3 314,269 - thrB MCAODC_01620 0.89 -1.5 314,269 - thrB MCAODC_01620 0.89 +1.8 314,269 - thrB MCAODC_01620 0.89 -0.2 314,269 - thrB MCAODC_01620 0.89 -1.3 314,269 - thrB MCAODC_01620 0.89 +0.9 314,269 - thrB MCAODC_01620 0.89 +0.8 314,269 - thrB MCAODC_01620 0.89 +0.2 314,269 - thrB MCAODC_01620 0.89 -0.6 314,269 - thrB MCAODC_01620 0.89 -2.2 314,269 - thrB MCAODC_01620 0.89 -1.1 314,336 + +0.2 314,337 - -0.4 314,384 - -1.9 314,384 - -0.2 314,401 - -1.1 314,401 - -0.2 314,401 - +0.2 314,404 + +0.1 314,404 + -1.9 314,405 - +2.0 314,405 - +1.8 314,405 - +0.1
Or see this region's nucleotide sequence