Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_00275

Experiment: bur MOI1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_00273 and OHPLBJKB_00274 are separated by 127 nucleotidesOHPLBJKB_00274 and OHPLBJKB_00275 are separated by 10 nucleotidesOHPLBJKB_00275 and OHPLBJKB_00276 are separated by 25 nucleotides OHPLBJKB_00273: OHPLBJKB_00273 - Maltodextrin phosphorylase, at 297,283 to 299,730 _00273 OHPLBJKB_00274: OHPLBJKB_00274 - hypothetical protein, at 299,858 to 300,196 _00274 OHPLBJKB_00275: OHPLBJKB_00275 - hypothetical protein, at 300,207 to 301,277 _00275 OHPLBJKB_00276: OHPLBJKB_00276 - hypothetical protein, at 301,303 to 302,295 _00276 Position (kb) 300 301 302Strain fitness (log2 ratio) -2 -1 0 1 2 3at 299.259 kb on + strand, within OHPLBJKB_00273at 299.298 kb on + strand, within OHPLBJKB_00273at 299.299 kb on - strand, within OHPLBJKB_00273at 299.349 kb on + strand, within OHPLBJKB_00273at 299.349 kb on + strand, within OHPLBJKB_00273at 299.350 kb on - strand, within OHPLBJKB_00273at 299.350 kb on - strand, within OHPLBJKB_00273at 299.350 kb on - strand, within OHPLBJKB_00273at 299.355 kb on + strand, within OHPLBJKB_00273at 299.419 kb on + strand, within OHPLBJKB_00273at 299.419 kb on + strand, within OHPLBJKB_00273at 299.420 kb on - strand, within OHPLBJKB_00273at 299.422 kb on + strand, within OHPLBJKB_00273at 299.423 kb on - strand, within OHPLBJKB_00273at 299.549 kb on + strandat 299.569 kb on + strandat 299.569 kb on + strandat 299.569 kb on + strandat 299.569 kb on + strandat 299.569 kb on + strandat 299.570 kb on - strandat 299.603 kb on - strandat 299.677 kb on - strandat 299.689 kb on + strandat 299.710 kb on - strandat 299.740 kb on + strandat 299.740 kb on + strandat 299.744 kb on + strandat 299.744 kb on + strandat 299.823 kb on + strandat 299.825 kb on + strandat 299.825 kb on + strandat 299.986 kb on + strand, within OHPLBJKB_00274at 299.986 kb on + strand, within OHPLBJKB_00274at 299.987 kb on - strand, within OHPLBJKB_00274at 300.051 kb on + strand, within OHPLBJKB_00274at 300.150 kb on + strand, within OHPLBJKB_00274at 300.185 kb on - strandat 300.199 kb on - strandat 300.216 kb on - strandat 300.311 kb on - strandat 300.364 kb on + strand, within OHPLBJKB_00275at 300.365 kb on - strand, within OHPLBJKB_00275at 300.368 kb on + strand, within OHPLBJKB_00275at 300.369 kb on - strand, within OHPLBJKB_00275at 300.404 kb on + strand, within OHPLBJKB_00275at 300.554 kb on - strand, within OHPLBJKB_00275at 300.560 kb on - strand, within OHPLBJKB_00275at 300.618 kb on + strand, within OHPLBJKB_00275at 300.620 kb on + strand, within OHPLBJKB_00275at 300.717 kb on + strand, within OHPLBJKB_00275at 301.007 kb on + strand, within OHPLBJKB_00275at 301.007 kb on + strand, within OHPLBJKB_00275at 301.007 kb on + strand, within OHPLBJKB_00275at 301.021 kb on + strand, within OHPLBJKB_00275at 301.097 kb on + strand, within OHPLBJKB_00275at 301.098 kb on - strand, within OHPLBJKB_00275at 301.098 kb on - strand, within OHPLBJKB_00275at 301.137 kb on + strand, within OHPLBJKB_00275at 301.248 kb on + strandat 301.332 kb on - strandat 301.332 kb on - strandat 301.372 kb on + strandat 301.375 kb on + strandat 301.375 kb on + strandat 301.376 kb on - strandat 301.376 kb on - strandat 301.378 kb on - strandat 301.378 kb on - strandat 301.436 kb on + strand, within OHPLBJKB_00276at 301.436 kb on + strand, within OHPLBJKB_00276at 301.437 kb on - strand, within OHPLBJKB_00276at 301.474 kb on + strand, within OHPLBJKB_00276at 301.490 kb on + strand, within OHPLBJKB_00276at 301.491 kb on - strand, within OHPLBJKB_00276at 301.491 kb on - strand, within OHPLBJKB_00276at 301.492 kb on + strand, within OHPLBJKB_00276at 301.492 kb on + strand, within OHPLBJKB_00276at 301.492 kb on + strand, within OHPLBJKB_00276at 301.514 kb on - strand, within OHPLBJKB_00276at 301.514 kb on - strand, within OHPLBJKB_00276at 301.518 kb on + strand, within OHPLBJKB_00276at 301.523 kb on + strand, within OHPLBJKB_00276at 301.523 kb on + strand, within OHPLBJKB_00276at 301.523 kb on + strand, within OHPLBJKB_00276at 301.524 kb on - strand, within OHPLBJKB_00276at 301.621 kb on - strand, within OHPLBJKB_00276at 301.750 kb on - strand, within OHPLBJKB_00276at 301.845 kb on + strand, within OHPLBJKB_00276at 301.846 kb on - strand, within OHPLBJKB_00276at 301.847 kb on + strand, within OHPLBJKB_00276at 301.848 kb on - strand, within OHPLBJKB_00276at 302.029 kb on + strand, within OHPLBJKB_00276at 302.029 kb on + strand, within OHPLBJKB_00276at 302.029 kb on + strand, within OHPLBJKB_00276at 302.030 kb on - strand, within OHPLBJKB_00276at 302.040 kb on + strand, within OHPLBJKB_00276at 302.053 kb on - strand, within OHPLBJKB_00276at 302.090 kb on + strand, within OHPLBJKB_00276at 302.091 kb on - strand, within OHPLBJKB_00276at 302.155 kb on + strand, within OHPLBJKB_00276at 302.158 kb on - strand, within OHPLBJKB_00276at 302.158 kb on - strand, within OHPLBJKB_00276at 302.213 kb on + strandat 302.213 kb on + strandat 302.214 kb on - strandat 302.251 kb on + strandat 302.251 kb on + strandat 302.252 kb on - strandat 302.263 kb on + strandat 302.264 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction bur MOI1
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299,259 + OHPLBJKB_00273 0.81 -0.9
299,298 + OHPLBJKB_00273 0.82 -2.2
299,299 - OHPLBJKB_00273 0.82 -0.1
299,349 + OHPLBJKB_00273 0.84 -1.0
299,349 + OHPLBJKB_00273 0.84 +0.3
299,350 - OHPLBJKB_00273 0.84 +0.6
299,350 - OHPLBJKB_00273 0.84 -0.5
299,350 - OHPLBJKB_00273 0.84 -0.0
299,355 + OHPLBJKB_00273 0.85 +0.6
299,419 + OHPLBJKB_00273 0.87 +3.3
299,419 + OHPLBJKB_00273 0.87 +2.5
299,420 - OHPLBJKB_00273 0.87 +1.1
299,422 + OHPLBJKB_00273 0.87 +1.4
299,423 - OHPLBJKB_00273 0.87 +0.1
299,549 + -2.1
299,569 + +1.7
299,569 + +0.9
299,569 + -0.8
299,569 + +0.5
299,569 + +0.3
299,570 - -1.3
299,603 - -0.1
299,677 - -0.3
299,689 + -2.4
299,710 - -0.8
299,740 + -2.7
299,740 + +0.5
299,744 + -2.0
299,744 + -1.3
299,823 + -0.3
299,825 + +0.1
299,825 + +1.1
299,986 + OHPLBJKB_00274 0.38 -1.6
299,986 + OHPLBJKB_00274 0.38 +0.9
299,987 - OHPLBJKB_00274 0.38 -0.5
300,051 + OHPLBJKB_00274 0.57 -1.1
300,150 + OHPLBJKB_00274 0.86 -2.4
300,185 - +0.9
300,199 - -1.2
300,216 - +0.5
300,311 - -0.4
300,364 + OHPLBJKB_00275 0.15 +0.8
300,365 - OHPLBJKB_00275 0.15 -1.0
300,368 + OHPLBJKB_00275 0.15 -2.4
300,369 - OHPLBJKB_00275 0.15 +2.1
300,404 + OHPLBJKB_00275 0.18 -0.8
300,554 - OHPLBJKB_00275 0.32 -0.8
300,560 - OHPLBJKB_00275 0.33 -1.3
300,618 + OHPLBJKB_00275 0.38 -1.3
300,620 + OHPLBJKB_00275 0.39 +0.2
300,717 + OHPLBJKB_00275 0.48 -0.5
301,007 + OHPLBJKB_00275 0.75 -0.1
301,007 + OHPLBJKB_00275 0.75 +0.2
301,007 + OHPLBJKB_00275 0.75 +2.3
301,021 + OHPLBJKB_00275 0.76 -1.0
301,097 + OHPLBJKB_00275 0.83 -0.3
301,098 - OHPLBJKB_00275 0.83 -0.7
301,098 - OHPLBJKB_00275 0.83 +1.2
301,137 + OHPLBJKB_00275 0.87 -0.8
301,248 + +1.0
301,332 - +0.5
301,332 - -2.0
301,372 + -1.8
301,375 + -0.5
301,375 + +2.3
301,376 - +0.7
301,376 - +0.3
301,378 - +0.6
301,378 - +0.0
301,436 + OHPLBJKB_00276 0.13 +0.3
301,436 + OHPLBJKB_00276 0.13 -1.3
301,437 - OHPLBJKB_00276 0.13 +0.1
301,474 + OHPLBJKB_00276 0.17 +0.9
301,490 + OHPLBJKB_00276 0.19 +0.5
301,491 - OHPLBJKB_00276 0.19 -1.3
301,491 - OHPLBJKB_00276 0.19 +1.7
301,492 + OHPLBJKB_00276 0.19 -1.8
301,492 + OHPLBJKB_00276 0.19 -0.4
301,492 + OHPLBJKB_00276 0.19 -0.1
301,514 - OHPLBJKB_00276 0.21 -1.8
301,514 - OHPLBJKB_00276 0.21 -0.5
301,518 + OHPLBJKB_00276 0.22 +0.9
301,523 + OHPLBJKB_00276 0.22 +0.2
301,523 + OHPLBJKB_00276 0.22 +0.2
301,523 + OHPLBJKB_00276 0.22 +0.5
301,524 - OHPLBJKB_00276 0.22 +2.5
301,621 - OHPLBJKB_00276 0.32 +1.1
301,750 - OHPLBJKB_00276 0.45 +0.9
301,845 + OHPLBJKB_00276 0.55 -1.8
301,846 - OHPLBJKB_00276 0.55 +0.1
301,847 + OHPLBJKB_00276 0.55 -2.5
301,848 - OHPLBJKB_00276 0.55 -0.2
302,029 + OHPLBJKB_00276 0.73 -0.7
302,029 + OHPLBJKB_00276 0.73 -0.9
302,029 + OHPLBJKB_00276 0.73 -0.4
302,030 - OHPLBJKB_00276 0.73 -0.1
302,040 + OHPLBJKB_00276 0.74 -1.1
302,053 - OHPLBJKB_00276 0.76 +1.2
302,090 + OHPLBJKB_00276 0.79 +0.3
302,091 - OHPLBJKB_00276 0.79 +0.3
302,155 + OHPLBJKB_00276 0.86 -0.8
302,158 - OHPLBJKB_00276 0.86 -0.6
302,158 - OHPLBJKB_00276 0.86 -1.3
302,213 + +0.8
302,213 + -0.6
302,214 - +1.2
302,251 + +0.1
302,251 + +1.2
302,252 - +1.5
302,263 + -2.4
302,264 - -0.5

Or see this region's nucleotide sequence