Experiment: R2A_PIPES pH 4.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moeB and OKGIIK_05250 are separated by 192 nucleotides OKGIIK_05250 and OKGIIK_05255 are separated by 4 nucleotides OKGIIK_05255 and moaA are separated by 247 nucleotides
OKGIIK_05245: moeB - molybdopterin-synthase adenylyltransferase MoeB, at 1,146,383 to 1,147,579
moeB
OKGIIK_05250: OKGIIK_05250 - hypothetical protein, at 1,147,772 to 1,148,080
_05250
OKGIIK_05255: OKGIIK_05255 - Ribonucleotide reductase subunit alpha, at 1,148,085 to 1,148,489
_05255
OKGIIK_05260: moaA - GTP 3',8-cyclase MoaA, at 1,148,737 to 1,149,717
moaA
Position (kb)
1147
1148
1149 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1146.786 kb on + strand, within moeB at 1146.786 kb on + strand, within moeB at 1146.787 kb on - strand, within moeB at 1147.047 kb on + strand, within moeB at 1147.048 kb on - strand, within moeB at 1147.048 kb on - strand, within moeB at 1147.067 kb on + strand, within moeB at 1147.067 kb on + strand, within moeB at 1147.068 kb on - strand, within moeB at 1147.085 kb on + strand, within moeB at 1147.086 kb on - strand, within moeB at 1147.107 kb on - strand, within moeB at 1147.107 kb on - strand, within moeB at 1147.158 kb on + strand, within moeB at 1147.198 kb on + strand, within moeB at 1147.247 kb on + strand, within moeB at 1147.247 kb on + strand, within moeB at 1147.247 kb on + strand, within moeB at 1147.248 kb on - strand, within moeB at 1147.248 kb on - strand, within moeB at 1147.248 kb on - strand, within moeB at 1147.313 kb on + strand, within moeB at 1147.313 kb on + strand, within moeB at 1147.313 kb on + strand, within moeB at 1147.313 kb on + strand, within moeB at 1147.313 kb on + strand, within moeB at 1147.313 kb on + strand, within moeB at 1147.314 kb on - strand, within moeB at 1147.314 kb on - strand, within moeB at 1147.314 kb on - strand, within moeB at 1147.314 kb on - strand, within moeB at 1147.314 kb on - strand, within moeB at 1147.314 kb on - strand, within moeB at 1147.314 kb on - strand, within moeB at 1147.314 kb on - strand, within moeB at 1147.433 kb on + strand, within moeB at 1147.496 kb on + strand at 1147.496 kb on + strand at 1147.617 kb on + strand at 1147.617 kb on + strand at 1147.618 kb on - strand at 1147.618 kb on - strand at 1147.663 kb on + strand at 1147.692 kb on - strand at 1147.712 kb on + strand at 1147.838 kb on + strand, within OKGIIK_05250 at 1147.838 kb on + strand, within OKGIIK_05250 at 1147.943 kb on + strand, within OKGIIK_05250 at 1147.943 kb on + strand, within OKGIIK_05250 at 1147.943 kb on + strand, within OKGIIK_05250 at 1147.943 kb on + strand, within OKGIIK_05250 at 1147.944 kb on - strand, within OKGIIK_05250 at 1148.047 kb on + strand, within OKGIIK_05250 at 1148.047 kb on + strand, within OKGIIK_05250 at 1148.047 kb on + strand, within OKGIIK_05250 at 1148.048 kb on - strand, within OKGIIK_05250 at 1148.048 kb on - strand, within OKGIIK_05250 at 1148.048 kb on - strand, within OKGIIK_05250 at 1148.078 kb on + strand at 1148.079 kb on - strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.100 kb on + strand at 1148.101 kb on - strand at 1148.101 kb on - strand at 1148.101 kb on - strand at 1148.101 kb on - strand at 1148.101 kb on - strand at 1148.101 kb on - strand at 1148.101 kb on - strand at 1148.262 kb on - strand, within OKGIIK_05255 at 1148.265 kb on + strand, within OKGIIK_05255 at 1148.266 kb on - strand, within OKGIIK_05255 at 1148.369 kb on + strand, within OKGIIK_05255 at 1148.370 kb on - strand, within OKGIIK_05255 at 1148.402 kb on - strand, within OKGIIK_05255 at 1148.421 kb on + strand, within OKGIIK_05255 at 1148.422 kb on - strand, within OKGIIK_05255 at 1148.482 kb on - strand at 1148.525 kb on + strand at 1148.525 kb on + strand at 1148.525 kb on + strand at 1148.615 kb on + strand at 1148.616 kb on - strand at 1148.621 kb on - strand at 1148.776 kb on + strand at 1148.776 kb on + strand at 1148.810 kb on - strand at 1148.810 kb on - strand at 1148.810 kb on - strand at 1148.821 kb on + strand at 1148.821 kb on + strand at 1148.821 kb on + strand at 1148.821 kb on + strand at 1148.821 kb on + strand at 1148.821 kb on + strand at 1148.821 kb on + strand at 1148.822 kb on - strand at 1148.822 kb on - strand at 1148.822 kb on - strand at 1148.822 kb on - strand at 1148.822 kb on - strand at 1148.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.5 remove 1,146,786 + moeB OKGIIK_05245 0.34 +1.3 1,146,786 + moeB OKGIIK_05245 0.34 -3.0 1,146,787 - moeB OKGIIK_05245 0.34 +0.6 1,147,047 + moeB OKGIIK_05245 0.55 +0.4 1,147,048 - moeB OKGIIK_05245 0.56 +0.7 1,147,048 - moeB OKGIIK_05245 0.56 -1.6 1,147,067 + moeB OKGIIK_05245 0.57 -1.0 1,147,067 + moeB OKGIIK_05245 0.57 -1.0 1,147,068 - moeB OKGIIK_05245 0.57 -0.6 1,147,085 + moeB OKGIIK_05245 0.59 +0.1 1,147,086 - moeB OKGIIK_05245 0.59 -0.7 1,147,107 - moeB OKGIIK_05245 0.60 +0.9 1,147,107 - moeB OKGIIK_05245 0.60 -1.1 1,147,158 + moeB OKGIIK_05245 0.65 +0.6 1,147,198 + moeB OKGIIK_05245 0.68 -1.0 1,147,247 + moeB OKGIIK_05245 0.72 -1.0 1,147,247 + moeB OKGIIK_05245 0.72 +1.0 1,147,247 + moeB OKGIIK_05245 0.72 -0.6 1,147,248 - moeB OKGIIK_05245 0.72 +0.5 1,147,248 - moeB OKGIIK_05245 0.72 -0.6 1,147,248 - moeB OKGIIK_05245 0.72 -0.5 1,147,313 + moeB OKGIIK_05245 0.78 +0.4 1,147,313 + moeB OKGIIK_05245 0.78 -0.1 1,147,313 + moeB OKGIIK_05245 0.78 -1.3 1,147,313 + moeB OKGIIK_05245 0.78 -0.4 1,147,313 + moeB OKGIIK_05245 0.78 +0.1 1,147,313 + moeB OKGIIK_05245 0.78 -0.4 1,147,314 - moeB OKGIIK_05245 0.78 -0.2 1,147,314 - moeB OKGIIK_05245 0.78 +0.1 1,147,314 - moeB OKGIIK_05245 0.78 +1.4 1,147,314 - moeB OKGIIK_05245 0.78 +0.2 1,147,314 - moeB OKGIIK_05245 0.78 +0.4 1,147,314 - moeB OKGIIK_05245 0.78 -0.4 1,147,314 - moeB OKGIIK_05245 0.78 +0.1 1,147,314 - moeB OKGIIK_05245 0.78 -2.7 1,147,433 + moeB OKGIIK_05245 0.88 +1.0 1,147,496 + -1.6 1,147,496 + -0.4 1,147,617 + +0.4 1,147,617 + -1.0 1,147,618 - -1.0 1,147,618 - -0.1 1,147,663 + -0.8 1,147,692 - +0.8 1,147,712 + -0.4 1,147,838 + OKGIIK_05250 0.21 -1.4 1,147,838 + OKGIIK_05250 0.21 +0.2 1,147,943 + OKGIIK_05250 0.55 +1.2 1,147,943 + OKGIIK_05250 0.55 -0.9 1,147,943 + OKGIIK_05250 0.55 -0.9 1,147,943 + OKGIIK_05250 0.55 +0.4 1,147,944 - OKGIIK_05250 0.56 -0.9 1,148,047 + OKGIIK_05250 0.89 -1.9 1,148,047 + OKGIIK_05250 0.89 -0.2 1,148,047 + OKGIIK_05250 0.89 -1.4 1,148,048 - OKGIIK_05250 0.89 +0.0 1,148,048 - OKGIIK_05250 0.89 -1.1 1,148,048 - OKGIIK_05250 0.89 -2.5 1,148,078 + +0.1 1,148,079 - -2.1 1,148,100 + -1.6 1,148,100 + -1.4 1,148,100 + +1.4 1,148,100 + -0.6 1,148,100 + +0.1 1,148,100 + +0.4 1,148,100 + -1.1 1,148,100 + -3.1 1,148,100 + +0.1 1,148,100 + -1.4 1,148,100 + -0.6 1,148,101 - +0.2 1,148,101 - +0.6 1,148,101 - -2.4 1,148,101 - -0.3 1,148,101 - -1.3 1,148,101 - -1.1 1,148,101 - -0.3 1,148,262 - OKGIIK_05255 0.44 +0.0 1,148,265 + OKGIIK_05255 0.44 +0.6 1,148,266 - OKGIIK_05255 0.45 -1.7 1,148,369 + OKGIIK_05255 0.70 -0.6 1,148,370 - OKGIIK_05255 0.70 -1.1 1,148,402 - OKGIIK_05255 0.78 -0.4 1,148,421 + OKGIIK_05255 0.83 -0.6 1,148,422 - OKGIIK_05255 0.83 +0.3 1,148,482 - +0.9 1,148,525 + -0.4 1,148,525 + -1.3 1,148,525 + +0.2 1,148,615 + +0.5 1,148,616 - +0.1 1,148,621 - +2.0 1,148,776 + -0.9 1,148,776 + -1.6 1,148,810 - +1.4 1,148,810 - -0.2 1,148,810 - -0.6 1,148,821 + -0.7 1,148,821 + -1.2 1,148,821 + -0.3 1,148,821 + -0.2 1,148,821 + -1.2 1,148,821 + +0.1 1,148,821 + -1.3 1,148,822 - -0.6 1,148,822 - -0.4 1,148,822 - -0.4 1,148,822 - -1.6 1,148,822 - -0.7 1,148,822 - -0.7
Or see this region's nucleotide sequence