Experiment: R2A_PIPES pH 4.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_01420 and OKGIIK_01425 are separated by 75 nucleotides OKGIIK_01425 and prfC are separated by 51 nucleotides
OKGIIK_01420: OKGIIK_01420 - Histidine kinase, at 314,667 to 318,299
_01420
OKGIIK_01425: OKGIIK_01425 - Abortive infection protein, at 318,375 to 319,274
_01425
OKGIIK_01430: prfC - peptide chain release factor 3, at 319,326 to 320,927
prfC
Position (kb) 318
319
320 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 317.474 kb on + strand, within OKGIIK_01420 at 317.475 kb on - strand, within OKGIIK_01420 at 317.496 kb on + strand, within OKGIIK_01420 at 317.496 kb on + strand, within OKGIIK_01420 at 317.496 kb on + strand, within OKGIIK_01420 at 317.497 kb on - strand, within OKGIIK_01420 at 317.497 kb on - strand, within OKGIIK_01420 at 317.497 kb on - strand, within OKGIIK_01420 at 317.497 kb on - strand, within OKGIIK_01420 at 317.628 kb on + strand, within OKGIIK_01420 at 317.629 kb on - strand, within OKGIIK_01420 at 317.629 kb on - strand, within OKGIIK_01420 at 317.629 kb on - strand, within OKGIIK_01420 at 317.818 kb on + strand, within OKGIIK_01420 at 317.818 kb on + strand, within OKGIIK_01420 at 317.870 kb on + strand, within OKGIIK_01420 at 317.870 kb on + strand, within OKGIIK_01420 at 317.871 kb on - strand, within OKGIIK_01420 at 318.139 kb on + strand at 318.139 kb on + strand at 318.139 kb on + strand at 318.139 kb on + strand at 318.139 kb on + strand at 318.139 kb on + strand at 318.139 kb on + strand at 318.139 kb on + strand at 318.139 kb on + strand at 318.140 kb on - strand at 318.140 kb on - strand at 318.140 kb on - strand at 318.140 kb on - strand at 318.140 kb on - strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.143 kb on + strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.144 kb on - strand at 318.311 kb on + strand at 318.312 kb on - strand at 318.312 kb on - strand at 318.341 kb on + strand at 318.341 kb on + strand at 318.341 kb on + strand at 318.341 kb on + strand at 318.342 kb on - strand at 318.342 kb on - strand at 318.342 kb on - strand at 318.342 kb on - strand at 318.342 kb on - strand at 318.342 kb on - strand at 318.342 kb on - strand at 318.342 kb on - strand at 318.342 kb on - strand at 318.342 kb on - strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.449 kb on + strand at 318.450 kb on - strand at 318.450 kb on - strand at 318.450 kb on - strand at 318.450 kb on - strand at 318.450 kb on - strand at 318.450 kb on - strand at 318.450 kb on - strand at 318.450 kb on - strand at 318.450 kb on - strand at 318.450 kb on - strand at 318.571 kb on + strand, within OKGIIK_01425 at 318.571 kb on + strand, within OKGIIK_01425 at 318.571 kb on + strand, within OKGIIK_01425 at 318.571 kb on + strand, within OKGIIK_01425 at 318.571 kb on + strand, within OKGIIK_01425 at 318.571 kb on + strand, within OKGIIK_01425 at 318.571 kb on + strand, within OKGIIK_01425 at 318.572 kb on - strand, within OKGIIK_01425 at 318.572 kb on - strand, within OKGIIK_01425 at 318.572 kb on - strand, within OKGIIK_01425 at 318.572 kb on - strand, within OKGIIK_01425 at 318.572 kb on - strand, within OKGIIK_01425 at 318.572 kb on - strand, within OKGIIK_01425 at 318.572 kb on - strand, within OKGIIK_01425 at 318.572 kb on - strand, within OKGIIK_01425 at 318.572 kb on - strand, within OKGIIK_01425 at 318.643 kb on + strand, within OKGIIK_01425 at 318.643 kb on + strand, within OKGIIK_01425 at 318.643 kb on + strand, within OKGIIK_01425 at 318.643 kb on + strand, within OKGIIK_01425 at 318.643 kb on + strand, within OKGIIK_01425 at 318.644 kb on - strand, within OKGIIK_01425 at 318.644 kb on - strand, within OKGIIK_01425 at 318.898 kb on + strand, within OKGIIK_01425 at 318.898 kb on + strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.899 kb on - strand, within OKGIIK_01425 at 318.913 kb on + strand, within OKGIIK_01425 at 318.914 kb on - strand, within OKGIIK_01425 at 318.914 kb on - strand, within OKGIIK_01425 at 318.914 kb on - strand, within OKGIIK_01425 at 318.914 kb on - strand, within OKGIIK_01425 at 318.914 kb on - strand, within OKGIIK_01425 at 318.914 kb on - strand, within OKGIIK_01425 at 318.914 kb on - strand, within OKGIIK_01425 at 318.914 kb on - strand, within OKGIIK_01425 at 318.914 kb on - strand, within OKGIIK_01425 at 319.029 kb on + strand, within OKGIIK_01425 at 319.042 kb on + strand, within OKGIIK_01425 at 319.042 kb on + strand, within OKGIIK_01425 at 319.042 kb on + strand, within OKGIIK_01425 at 319.042 kb on + strand, within OKGIIK_01425 at 319.042 kb on + strand, within OKGIIK_01425 at 319.042 kb on + strand, within OKGIIK_01425 at 319.042 kb on + strand, within OKGIIK_01425 at 319.042 kb on + strand, within OKGIIK_01425 at 319.305 kb on - strand at 319.305 kb on - strand at 319.327 kb on + strand at 319.327 kb on + strand at 319.328 kb on - strand at 319.328 kb on - strand at 319.328 kb on - strand at 319.328 kb on - strand at 319.328 kb on - strand at 319.328 kb on - strand at 319.328 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.5 remove 317,474 + OKGIIK_01420 0.77 -1.0 317,475 - OKGIIK_01420 0.77 -2.0 317,496 + OKGIIK_01420 0.78 -0.9 317,496 + OKGIIK_01420 0.78 +0.8 317,496 + OKGIIK_01420 0.78 +0.9 317,497 - OKGIIK_01420 0.78 +0.5 317,497 - OKGIIK_01420 0.78 +1.3 317,497 - OKGIIK_01420 0.78 -1.7 317,497 - OKGIIK_01420 0.78 -0.3 317,628 + OKGIIK_01420 0.82 +0.1 317,629 - OKGIIK_01420 0.82 +0.4 317,629 - OKGIIK_01420 0.82 -0.5 317,629 - OKGIIK_01420 0.82 -2.2 317,818 + OKGIIK_01420 0.87 +1.2 317,818 + OKGIIK_01420 0.87 -0.6 317,870 + OKGIIK_01420 0.88 +1.0 317,870 + OKGIIK_01420 0.88 -1.0 317,871 - OKGIIK_01420 0.88 -0.3 318,139 + +0.9 318,139 + +0.3 318,139 + +0.3 318,139 + -2.0 318,139 + -0.2 318,139 + -2.0 318,139 + -1.2 318,139 + -0.5 318,139 + +0.4 318,140 - +0.3 318,140 - -0.0 318,140 - +0.1 318,140 - +0.7 318,140 - +0.0 318,143 + -0.1 318,143 + +1.1 318,143 + -0.4 318,143 + -3.0 318,143 + +0.9 318,143 + +0.3 318,143 + -0.3 318,143 + -1.5 318,143 + +1.0 318,143 + +0.5 318,143 + -0.5 318,143 + +0.1 318,143 + +0.4 318,143 + -0.6 318,143 + -0.4 318,143 + +0.7 318,143 + -0.0 318,143 + -2.3 318,143 + -0.3 318,144 - +0.5 318,144 - +0.1 318,144 - -0.3 318,144 - -0.7 318,144 - -0.6 318,144 - +0.2 318,144 - +1.3 318,144 - +0.1 318,144 - -0.4 318,144 - +1.0 318,144 - -2.6 318,144 - -1.6 318,144 - -1.1 318,144 - +0.5 318,144 - -1.0 318,144 - +1.0 318,144 - -0.7 318,144 - -0.9 318,311 + -0.1 318,312 - +0.7 318,312 - +0.4 318,341 + +0.1 318,341 + +1.1 318,341 + -0.3 318,341 + -0.5 318,342 - -0.9 318,342 - -1.7 318,342 - -1.3 318,342 - -1.3 318,342 - -1.4 318,342 - -1.4 318,342 - +1.3 318,342 - -0.0 318,342 - +0.4 318,342 - -1.3 318,449 + +0.3 318,449 + +0.3 318,449 + -2.6 318,449 + -0.7 318,449 + -0.1 318,449 + +0.5 318,449 + -0.7 318,449 + -0.6 318,449 + -0.0 318,449 + +0.2 318,449 + -0.4 318,450 - -0.9 318,450 - +0.9 318,450 - +1.1 318,450 - -0.4 318,450 - +0.3 318,450 - -1.1 318,450 - -0.6 318,450 - -0.9 318,450 - -0.9 318,450 - -1.4 318,571 + OKGIIK_01425 0.22 -1.9 318,571 + OKGIIK_01425 0.22 -1.6 318,571 + OKGIIK_01425 0.22 -1.7 318,571 + OKGIIK_01425 0.22 -1.1 318,571 + OKGIIK_01425 0.22 +1.1 318,571 + OKGIIK_01425 0.22 -1.1 318,571 + OKGIIK_01425 0.22 +1.0 318,572 - OKGIIK_01425 0.22 -1.5 318,572 - OKGIIK_01425 0.22 +0.6 318,572 - OKGIIK_01425 0.22 -0.9 318,572 - OKGIIK_01425 0.22 +0.3 318,572 - OKGIIK_01425 0.22 +0.5 318,572 - OKGIIK_01425 0.22 -1.6 318,572 - OKGIIK_01425 0.22 +0.2 318,572 - OKGIIK_01425 0.22 +0.9 318,572 - OKGIIK_01425 0.22 +0.1 318,643 + OKGIIK_01425 0.30 +0.4 318,643 + OKGIIK_01425 0.30 -0.1 318,643 + OKGIIK_01425 0.30 -2.2 318,643 + OKGIIK_01425 0.30 -1.7 318,643 + OKGIIK_01425 0.30 -0.2 318,644 - OKGIIK_01425 0.30 +0.1 318,644 - OKGIIK_01425 0.30 +0.5 318,898 + OKGIIK_01425 0.58 -1.0 318,898 + OKGIIK_01425 0.58 +1.1 318,899 - OKGIIK_01425 0.58 -1.0 318,899 - OKGIIK_01425 0.58 +0.2 318,899 - OKGIIK_01425 0.58 +1.0 318,899 - OKGIIK_01425 0.58 -0.9 318,899 - OKGIIK_01425 0.58 -0.4 318,899 - OKGIIK_01425 0.58 +0.2 318,899 - OKGIIK_01425 0.58 -2.0 318,899 - OKGIIK_01425 0.58 +0.3 318,899 - OKGIIK_01425 0.58 +0.8 318,899 - OKGIIK_01425 0.58 -0.0 318,899 - OKGIIK_01425 0.58 -0.6 318,899 - OKGIIK_01425 0.58 -0.1 318,899 - OKGIIK_01425 0.58 -1.7 318,899 - OKGIIK_01425 0.58 -0.3 318,899 - OKGIIK_01425 0.58 -1.6 318,899 - OKGIIK_01425 0.58 -0.7 318,899 - OKGIIK_01425 0.58 -0.0 318,913 + OKGIIK_01425 0.60 -0.0 318,914 - OKGIIK_01425 0.60 +1.6 318,914 - OKGIIK_01425 0.60 +0.9 318,914 - OKGIIK_01425 0.60 +0.3 318,914 - OKGIIK_01425 0.60 -0.4 318,914 - OKGIIK_01425 0.60 -0.1 318,914 - OKGIIK_01425 0.60 +0.3 318,914 - OKGIIK_01425 0.60 -1.2 318,914 - OKGIIK_01425 0.60 +0.7 318,914 - OKGIIK_01425 0.60 -0.5 319,029 + OKGIIK_01425 0.73 -0.2 319,042 + OKGIIK_01425 0.74 -0.0 319,042 + OKGIIK_01425 0.74 -1.0 319,042 + OKGIIK_01425 0.74 +0.4 319,042 + OKGIIK_01425 0.74 +0.3 319,042 + OKGIIK_01425 0.74 -0.1 319,042 + OKGIIK_01425 0.74 +1.3 319,042 + OKGIIK_01425 0.74 -0.7 319,042 + OKGIIK_01425 0.74 +0.5 319,305 - +1.0 319,305 - -0.3 319,327 + -0.1 319,327 + +2.1 319,328 - -0.3 319,328 - +0.2 319,328 - +0.3 319,328 - +1.5 319,328 - -1.7 319,328 - +0.6 319,328 - -1.4
Or see this region's nucleotide sequence