Experiment: D-Glucose and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1 and GFF2 are separated by 212 nucleotides GFF2 and GFF3 are separated by 3 nucleotides
GFF1 - Tyrosine--tRNA ligase, at 304 to 1,503
GFF1
GFF2 - hypothetical protein, at 1,716 to 3,140
GFF2
GFF3 - Anhydro-N-acetylmuramic acid kinase, at 3,144 to 4,235
GFF3
Position (kb)
1
2
3
4 Strain fitness (log2 ratio)
-1
0
1 at 1.612 kb on - strand at 1.653 kb on - strand at 1.693 kb on + strand at 1.693 kb on + strand at 1.694 kb on - strand at 1.694 kb on - strand at 1.711 kb on + strand at 1.711 kb on + strand at 1.712 kb on - strand at 1.715 kb on + strand at 1.715 kb on + strand at 1.715 kb on + strand at 1.715 kb on + strand at 1.716 kb on - strand at 1.716 kb on - strand at 1.716 kb on - strand at 1.716 kb on - strand at 1.716 kb on - strand at 1.716 kb on - strand at 1.813 kb on + strand at 1.813 kb on + strand at 2.012 kb on - strand, within GFF2 at 2.073 kb on - strand, within GFF2 at 2.082 kb on - strand, within GFF2 at 2.082 kb on - strand, within GFF2 at 2.129 kb on - strand, within GFF2 at 2.129 kb on - strand, within GFF2 at 2.325 kb on + strand, within GFF2 at 2.325 kb on + strand, within GFF2 at 2.326 kb on - strand, within GFF2 at 2.326 kb on - strand, within GFF2 at 2.326 kb on - strand, within GFF2 at 2.345 kb on + strand, within GFF2 at 2.345 kb on + strand, within GFF2 at 2.345 kb on + strand, within GFF2 at 2.345 kb on + strand, within GFF2 at 2.346 kb on - strand, within GFF2 at 2.346 kb on - strand, within GFF2 at 2.346 kb on - strand, within GFF2 at 2.346 kb on - strand, within GFF2 at 2.346 kb on - strand, within GFF2 at 2.346 kb on - strand, within GFF2 at 2.475 kb on + strand, within GFF2 at 2.475 kb on + strand, within GFF2 at 2.475 kb on + strand, within GFF2 at 2.476 kb on - strand, within GFF2 at 2.476 kb on - strand, within GFF2 at 2.556 kb on + strand, within GFF2 at 2.556 kb on + strand, within GFF2 at 2.556 kb on + strand, within GFF2 at 2.557 kb on - strand, within GFF2 at 2.557 kb on - strand, within GFF2 at 2.598 kb on + strand, within GFF2 at 2.598 kb on + strand, within GFF2 at 2.598 kb on + strand, within GFF2 at 2.598 kb on + strand, within GFF2 at 2.598 kb on + strand, within GFF2 at 2.598 kb on + strand, within GFF2 at 2.599 kb on - strand, within GFF2 at 2.599 kb on - strand, within GFF2 at 2.599 kb on - strand, within GFF2 at 2.599 kb on - strand, within GFF2 at 2.599 kb on - strand, within GFF2 at 2.599 kb on - strand, within GFF2 at 2.599 kb on - strand, within GFF2 at 2.599 kb on - strand, within GFF2 at 2.599 kb on - strand, within GFF2 at 2.601 kb on + strand, within GFF2 at 2.601 kb on + strand, within GFF2 at 2.601 kb on + strand, within GFF2 at 2.601 kb on + strand, within GFF2 at 2.601 kb on + strand, within GFF2 at 2.602 kb on - strand, within GFF2 at 2.602 kb on - strand, within GFF2 at 2.700 kb on - strand, within GFF2 at 2.736 kb on + strand, within GFF2 at 2.737 kb on - strand, within GFF2 at 2.847 kb on + strand, within GFF2 at 2.847 kb on + strand, within GFF2 at 2.847 kb on + strand, within GFF2 at 2.848 kb on - strand, within GFF2 at 2.848 kb on - strand, within GFF2 at 2.852 kb on + strand, within GFF2 at 2.853 kb on - strand, within GFF2 at 2.853 kb on - strand, within GFF2 at 2.853 kb on - strand, within GFF2 at 2.853 kb on - strand, within GFF2 at 2.859 kb on + strand, within GFF2 at 2.859 kb on + strand, within GFF2 at 2.859 kb on + strand, within GFF2 at 2.860 kb on - strand, within GFF2 at 2.860 kb on - strand, within GFF2 at 2.867 kb on + strand, within GFF2 at 2.867 kb on + strand, within GFF2 at 2.867 kb on + strand, within GFF2 at 2.867 kb on + strand, within GFF2 at 2.867 kb on + strand, within GFF2 at 2.931 kb on + strand, within GFF2 at 2.932 kb on - strand, within GFF2 at 2.934 kb on - strand, within GFF2 at 2.939 kb on + strand, within GFF2 at 2.939 kb on + strand, within GFF2 at 2.939 kb on + strand, within GFF2 at 2.939 kb on + strand, within GFF2 at 2.940 kb on - strand, within GFF2 at 2.940 kb on - strand, within GFF2 at 2.974 kb on - strand, within GFF2 at 2.974 kb on - strand, within GFF2 at 2.974 kb on - strand, within GFF2 at 2.974 kb on - strand, within GFF2 at 3.153 kb on + strand at 3.153 kb on + strand at 3.153 kb on + strand at 3.153 kb on + strand at 3.153 kb on + strand at 3.153 kb on + strand at 3.153 kb on + strand at 3.154 kb on - strand at 3.154 kb on - strand at 3.154 kb on - strand at 3.154 kb on - strand at 3.154 kb on - strand at 3.154 kb on - strand at 3.154 kb on - strand at 3.243 kb on + strand at 3.244 kb on - strand at 3.461 kb on + strand, within GFF3 at 3.476 kb on + strand, within GFF3 at 3.507 kb on - strand, within GFF3 at 3.507 kb on - strand, within GFF3 at 3.509 kb on + strand, within GFF3 at 3.571 kb on + strand, within GFF3 at 3.606 kb on - strand, within GFF3 at 3.606 kb on - strand, within GFF3 at 3.606 kb on - strand, within GFF3 at 3.606 kb on - strand, within GFF3 at 3.606 kb on - strand, within GFF3 at 3.612 kb on - strand, within GFF3 at 3.676 kb on + strand, within GFF3 at 3.747 kb on + strand, within GFF3 at 3.747 kb on + strand, within GFF3 at 3.747 kb on + strand, within GFF3 at 3.747 kb on + strand, within GFF3 at 3.747 kb on + strand, within GFF3 at 3.748 kb on - strand, within GFF3 at 3.973 kb on + strand, within GFF3 at 4.071 kb on - strand, within GFF3 at 4.071 kb on - strand, within GFF3 at 4.117 kb on + strand, within GFF3 at 4.118 kb on - strand, within GFF3 at 4.118 kb on - strand, within GFF3
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose and casaminos (C) remove 1,612 - +0.6 1,653 - +0.1 1,693 + +0.0 1,693 + -0.0 1,694 - -0.4 1,694 - -0.3 1,711 + +0.9 1,711 + +0.1 1,712 - +0.4 1,715 + +1.5 1,715 + -0.7 1,715 + +0.1 1,715 + +0.2 1,716 - -0.7 1,716 - -0.5 1,716 - +1.1 1,716 - -0.9 1,716 - +0.5 1,716 - -0.9 1,813 + +0.0 1,813 + +0.2 2,012 - GFF2 0.21 -0.2 2,073 - GFF2 0.25 +0.2 2,082 - GFF2 0.26 -0.4 2,082 - GFF2 0.26 -0.3 2,129 - GFF2 0.29 +1.3 2,129 - GFF2 0.29 +0.6 2,325 + GFF2 0.43 +0.0 2,325 + GFF2 0.43 -0.3 2,326 - GFF2 0.43 -0.8 2,326 - GFF2 0.43 +0.2 2,326 - GFF2 0.43 -0.3 2,345 + GFF2 0.44 -0.2 2,345 + GFF2 0.44 +0.1 2,345 + GFF2 0.44 -0.6 2,345 + GFF2 0.44 -0.1 2,346 - GFF2 0.44 +0.3 2,346 - GFF2 0.44 -0.5 2,346 - GFF2 0.44 -0.4 2,346 - GFF2 0.44 +1.4 2,346 - GFF2 0.44 -1.1 2,346 - GFF2 0.44 -0.6 2,475 + GFF2 0.53 -0.2 2,475 + GFF2 0.53 -0.5 2,475 + GFF2 0.53 +0.2 2,476 - GFF2 0.53 +0.1 2,476 - GFF2 0.53 +0.7 2,556 + GFF2 0.59 -0.2 2,556 + GFF2 0.59 +0.2 2,556 + GFF2 0.59 -0.6 2,557 - GFF2 0.59 -0.2 2,557 - GFF2 0.59 +0.0 2,598 + GFF2 0.62 -0.3 2,598 + GFF2 0.62 -1.1 2,598 + GFF2 0.62 +0.6 2,598 + GFF2 0.62 +0.3 2,598 + GFF2 0.62 -0.7 2,598 + GFF2 0.62 +0.4 2,599 - GFF2 0.62 +0.5 2,599 - GFF2 0.62 +0.6 2,599 - GFF2 0.62 +0.5 2,599 - GFF2 0.62 +0.4 2,599 - GFF2 0.62 -0.6 2,599 - GFF2 0.62 +0.5 2,599 - GFF2 0.62 -0.1 2,599 - GFF2 0.62 -0.9 2,599 - GFF2 0.62 +0.4 2,601 + GFF2 0.62 -0.3 2,601 + GFF2 0.62 +0.2 2,601 + GFF2 0.62 +0.0 2,601 + GFF2 0.62 +0.5 2,601 + GFF2 0.62 -0.2 2,602 - GFF2 0.62 -0.3 2,602 - GFF2 0.62 +0.1 2,700 - GFF2 0.69 +0.1 2,736 + GFF2 0.72 +0.9 2,737 - GFF2 0.72 -0.2 2,847 + GFF2 0.79 -0.4 2,847 + GFF2 0.79 +1.0 2,847 + GFF2 0.79 -0.0 2,848 - GFF2 0.79 +1.0 2,848 - GFF2 0.79 +0.3 2,852 + GFF2 0.80 -0.8 2,853 - GFF2 0.80 -0.7 2,853 - GFF2 0.80 +0.7 2,853 - GFF2 0.80 +0.3 2,853 - GFF2 0.80 +0.2 2,859 + GFF2 0.80 +0.3 2,859 + GFF2 0.80 -0.6 2,859 + GFF2 0.80 +0.6 2,860 - GFF2 0.80 -0.1 2,860 - GFF2 0.80 +0.9 2,867 + GFF2 0.81 -0.4 2,867 + GFF2 0.81 -1.5 2,867 + GFF2 0.81 -1.4 2,867 + GFF2 0.81 +0.0 2,867 + GFF2 0.81 +1.2 2,931 + GFF2 0.85 +1.2 2,932 - GFF2 0.85 +0.7 2,934 - GFF2 0.85 -0.8 2,939 + GFF2 0.86 -0.6 2,939 + GFF2 0.86 +0.4 2,939 + GFF2 0.86 +1.0 2,939 + GFF2 0.86 +0.0 2,940 - GFF2 0.86 +0.0 2,940 - GFF2 0.86 +0.2 2,974 - GFF2 0.88 +0.6 2,974 - GFF2 0.88 +0.8 2,974 - GFF2 0.88 +0.3 2,974 - GFF2 0.88 +0.0 3,153 + -0.4 3,153 + +0.0 3,153 + +0.2 3,153 + +0.0 3,153 + +0.8 3,153 + -0.2 3,153 + +0.9 3,154 - -1.6 3,154 - -0.0 3,154 - -0.1 3,154 - +0.4 3,154 - -0.4 3,154 - +0.5 3,154 - -0.7 3,243 + +1.1 3,244 - +0.1 3,461 + GFF3 0.29 +0.9 3,476 + GFF3 0.30 +0.3 3,507 - GFF3 0.33 +0.6 3,507 - GFF3 0.33 -0.6 3,509 + GFF3 0.33 +0.1 3,571 + GFF3 0.39 +0.8 3,606 - GFF3 0.42 +0.0 3,606 - GFF3 0.42 +0.4 3,606 - GFF3 0.42 -0.4 3,606 - GFF3 0.42 -0.6 3,606 - GFF3 0.42 -1.0 3,612 - GFF3 0.43 +0.9 3,676 + GFF3 0.49 +0.2 3,747 + GFF3 0.55 +0.6 3,747 + GFF3 0.55 -0.4 3,747 + GFF3 0.55 +0.8 3,747 + GFF3 0.55 -1.7 3,747 + GFF3 0.55 +0.2 3,748 - GFF3 0.55 -0.3 3,973 + GFF3 0.76 +0.3 4,071 - GFF3 0.85 -1.1 4,071 - GFF3 0.85 -0.7 4,117 + GFF3 0.89 +0.1 4,118 - GFF3 0.89 -0.4 4,118 - GFF3 0.89 +0.1
Or see this region's nucleotide sequence