Strain Fitness in Escherichia coli ECRC102 around NIAGMN_25940

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntldhA and hslJ are separated by 110 nucleotideshslJ and ydbJ overlap by 4 nucleotidesydbJ and nifJ are separated by 273 nucleotides NIAGMN_25930: ldhA - D-lactate dehydrogenase, at 5,065,556 to 5,066,545 ldhA NIAGMN_25935: hslJ - heat shock protein HslJ, at 5,066,656 to 5,067,078 hslJ NIAGMN_25940: ydbJ - Uncharacterized protein YdbJ, at 5,067,075 to 5,067,341 ydbJ NIAGMN_25945: nifJ - pyruvate:ferredoxin (flavodoxin) oxidoreductase, at 5,067,615 to 5,071,139 nifJ Position (kb) 5067 5068Strain fitness (log2 ratio) -2 -1 0 1at 5066.118 kb on - strand, within ldhAat 5066.248 kb on + strand, within ldhAat 5066.499 kb on + strandat 5066.708 kb on + strand, within hslJat 5067.047 kb on + strandat 5067.048 kb on - strandat 5067.055 kb on - strandat 5067.055 kb on - strandat 5067.385 kb on + strandat 5067.557 kb on - strandat 5067.559 kb on + strandat 5067.603 kb on + strandat 5068.172 kb on + strand, within nifJat 5068.309 kb on - strand, within nifJ

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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5,066,118 - ldhA NIAGMN_25930 0.57 -0.1
5,066,248 + ldhA NIAGMN_25930 0.70 +0.3
5,066,499 + -0.4
5,066,708 + hslJ NIAGMN_25935 0.12 +0.9
5,067,047 + +1.5
5,067,048 - -0.1
5,067,055 - -2.7
5,067,055 - +0.3
5,067,385 + -0.1
5,067,557 - -0.6
5,067,559 + +1.3
5,067,603 + +0.1
5,068,172 + nifJ NIAGMN_25945 0.16 -1.1
5,068,309 - nifJ NIAGMN_25945 0.20 +0.0

Or see this region's nucleotide sequence