Strain Fitness in Escherichia coli ECRC102 around NIAGMN_25895

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynbB and ynbA overlap by 1 nucleotidesynbA and ydbD are separated by 170 nucleotidesydbD and pdxI are separated by 63 nucleotides NIAGMN_25885: ynbB - Uncharacterized protein YnbB, at 5,050,946 to 5,051,842 ynbB NIAGMN_25890: ynbA - Inner membrane protein YnbA, at 5,051,842 to 5,052,447 ynbA NIAGMN_25895: ydbD - Uncharacterized protein YdbD, at 5,052,618 to 5,054,924 ydbD NIAGMN_25900: pdxI - pyridoxine 4-dehydrogenase, at 5,054,988 to 5,055,848 pdxI Position (kb) 5052 5053 5054 5055Strain fitness (log2 ratio) -1 0 1at 5051.738 kb on - strand, within ynbBat 5052.505 kb on - strandat 5052.936 kb on + strand, within ydbDat 5053.543 kb on + strand, within ydbDat 5053.544 kb on - strand, within ydbDat 5053.852 kb on - strand, within ydbDat 5054.004 kb on - strand, within ydbDat 5054.624 kb on + strand, within ydbDat 5054.924 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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5,051,738 - ynbB NIAGMN_25885 0.88 +0.9
5,052,505 - +0.2
5,052,936 + ydbD NIAGMN_25895 0.14 -0.7
5,053,543 + ydbD NIAGMN_25895 0.40 -0.7
5,053,544 - ydbD NIAGMN_25895 0.40 -0.1
5,053,852 - ydbD NIAGMN_25895 0.53 -0.1
5,054,004 - ydbD NIAGMN_25895 0.60 +0.1
5,054,624 + ydbD NIAGMN_25895 0.87 -0.8
5,054,924 + -1.6

Or see this region's nucleotide sequence