Strain Fitness in Escherichia coli ECRC102 around NIAGMN_24900

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmnmA and nudJ are separated by 53 nucleotidesnudJ and rluE are separated by 9 nucleotidesrluE and icd are separated by 171 nucleotides NIAGMN_24890: mnmA - tRNA 2-thiouridine(34) synthase MnmA, at 4,848,137 to 4,849,243 mnmA NIAGMN_24895: nudJ - phosphatase NudJ, at 4,849,297 to 4,849,758 nudJ NIAGMN_24900: rluE - 23S rRNA pseudouridine(2457) synthase RluE, at 4,849,768 to 4,850,421 rluE NIAGMN_24905: icd - NADP-dependent isocitrate dehydrogenase, at 4,850,593 to 4,851,843 icd Position (kb) 4849 4850 4851Strain fitness (log2 ratio) -2 -1 0 1 2at 4849.371 kb on - strand, within nudJat 4849.678 kb on - strand, within nudJat 4849.727 kb on - strandat 4849.770 kb on - strandat 4849.770 kb on - strandat 4849.894 kb on - strand, within rluEat 4849.894 kb on - strand, within rluEat 4849.904 kb on + strand, within rluEat 4849.905 kb on - strand, within rluEat 4849.905 kb on - strand, within rluEat 4849.905 kb on - strand, within rluEat 4849.905 kb on - strand, within rluEat 4849.907 kb on - strand, within rluEat 4849.907 kb on - strand, within rluEat 4849.907 kb on - strand, within rluEat 4850.036 kb on - strand, within rluEat 4850.279 kb on + strand, within rluEat 4850.394 kb on + strandat 4850.424 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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4,849,371 - nudJ NIAGMN_24895 0.16 -0.9
4,849,678 - nudJ NIAGMN_24895 0.82 -0.7
4,849,727 - -0.1
4,849,770 - +1.9
4,849,770 - +0.9
4,849,894 - rluE NIAGMN_24900 0.19 -0.4
4,849,894 - rluE NIAGMN_24900 0.19 +0.4
4,849,904 + rluE NIAGMN_24900 0.21 +1.9
4,849,905 - rluE NIAGMN_24900 0.21 -0.5
4,849,905 - rluE NIAGMN_24900 0.21 +0.0
4,849,905 - rluE NIAGMN_24900 0.21 +1.9
4,849,905 - rluE NIAGMN_24900 0.21 -1.7
4,849,907 - rluE NIAGMN_24900 0.21 -1.9
4,849,907 - rluE NIAGMN_24900 0.21 -0.1
4,849,907 - rluE NIAGMN_24900 0.21 +1.3
4,850,036 - rluE NIAGMN_24900 0.41 +0.9
4,850,279 + rluE NIAGMN_24900 0.78 -0.4
4,850,394 + -1.5
4,850,424 + +1.1

Or see this region's nucleotide sequence