Strain Fitness in Escherichia coli ECRC102 around NIAGMN_24220

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrluC and yceF are separated by 100 nucleotidesyceF and yceD are separated by 159 nucleotidesyceD and rpmF are separated by 51 nucleotides NIAGMN_24215: rluC - 23S rRNA pseudouridine(955/2504/2580) synthase RluC, at 4,737,788 to 4,738,747 rluC NIAGMN_24220: yceF - 7-methyl-GTP pyrophosphatase, at 4,738,848 to 4,739,471 yceF NIAGMN_24230: yceD - 23S rRNA accumulation protein YceD, at 4,739,631 to 4,740,152 yceD NIAGMN_24235: rpmF - 50S ribosomal protein L32, at 4,740,204 to 4,740,377 rpmF Position (kb) 4738 4739 4740Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4737.850 kb on + strandat 4737.949 kb on + strand, within rluCat 4738.074 kb on + strand, within rluCat 4738.074 kb on + strand, within rluCat 4738.076 kb on + strand, within rluCat 4738.125 kb on + strand, within rluCat 4738.489 kb on + strand, within rluCat 4738.528 kb on + strand, within rluCat 4738.535 kb on + strand, within rluCat 4738.756 kb on + strandat 4738.756 kb on + strandat 4738.756 kb on + strandat 4738.756 kb on + strandat 4738.757 kb on - strandat 4738.758 kb on + strandat 4738.758 kb on + strandat 4738.758 kb on + strandat 4738.758 kb on + strandat 4738.758 kb on + strandat 4738.758 kb on + strandat 4738.758 kb on + strandat 4738.758 kb on + strandat 4738.759 kb on - strandat 4738.759 kb on - strandat 4738.784 kb on + strandat 4738.882 kb on - strandat 4738.926 kb on - strand, within yceFat 4738.980 kb on - strand, within yceFat 4739.021 kb on - strand, within yceFat 4739.024 kb on + strand, within yceFat 4739.221 kb on - strand, within yceFat 4739.432 kb on + strandat 4739.449 kb on + strandat 4739.451 kb on + strandat 4739.451 kb on + strandat 4739.475 kb on - strandat 4739.480 kb on - strandat 4739.647 kb on + strandat 4739.702 kb on + strand, within yceDat 4739.702 kb on + strand, within yceDat 4739.702 kb on + strand, within yceDat 4739.706 kb on + strand, within yceDat 4739.706 kb on + strand, within yceDat 4739.706 kb on + strand, within yceDat 4739.706 kb on + strand, within yceDat 4739.870 kb on + strand, within yceDat 4739.883 kb on + strand, within yceDat 4739.883 kb on + strand, within yceDat 4739.883 kb on + strand, within yceDat 4739.892 kb on + strand, within yceDat 4739.892 kb on + strand, within yceDat 4739.892 kb on + strand, within yceDat 4739.946 kb on + strand, within yceDat 4739.946 kb on + strand, within yceDat 4740.028 kb on + strand, within yceDat 4740.127 kb on + strandat 4740.139 kb on + strandat 4740.146 kb on + strandat 4740.150 kb on + strandat 4740.150 kb on + strandat 4740.150 kb on + strandat 4740.185 kb on + strandat 4740.345 kb on + strand, within rpmFat 4740.348 kb on + strand, within rpmFat 4740.371 kb on + strandat 4740.371 kb on + strandat 4740.375 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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4,737,850 + +0.8
4,737,949 + rluC NIAGMN_24215 0.17 +2.0
4,738,074 + rluC NIAGMN_24215 0.30 +2.0
4,738,074 + rluC NIAGMN_24215 0.30 +0.8
4,738,076 + rluC NIAGMN_24215 0.30 +1.0
4,738,125 + rluC NIAGMN_24215 0.35 +3.0
4,738,489 + rluC NIAGMN_24215 0.73 +0.4
4,738,528 + rluC NIAGMN_24215 0.77 +2.0
4,738,535 + rluC NIAGMN_24215 0.78 -1.5
4,738,756 + +0.4
4,738,756 + -0.0
4,738,756 + -0.8
4,738,756 + +0.2
4,738,757 - +0.6
4,738,758 + -2.7
4,738,758 + +0.4
4,738,758 + +0.1
4,738,758 + -1.0
4,738,758 + -1.0
4,738,758 + -2.5
4,738,758 + -0.8
4,738,758 + +1.0
4,738,759 - -0.8
4,738,759 - +0.5
4,738,784 + +3.0
4,738,882 - +0.4
4,738,926 - yceF NIAGMN_24220 0.12 +1.2
4,738,980 - yceF NIAGMN_24220 0.21 +1.4
4,739,021 - yceF NIAGMN_24220 0.28 -2.7
4,739,024 + yceF NIAGMN_24220 0.28 +0.2
4,739,221 - yceF NIAGMN_24220 0.60 -0.0
4,739,432 + -1.1
4,739,449 + -1.0
4,739,451 + -0.6
4,739,451 + -1.7
4,739,475 - -0.0
4,739,480 - -1.6
4,739,647 + -0.4
4,739,702 + yceD NIAGMN_24230 0.14 -0.5
4,739,702 + yceD NIAGMN_24230 0.14 +2.7
4,739,702 + yceD NIAGMN_24230 0.14 +1.1
4,739,706 + yceD NIAGMN_24230 0.14 -1.4
4,739,706 + yceD NIAGMN_24230 0.14 +0.4
4,739,706 + yceD NIAGMN_24230 0.14 -0.6
4,739,706 + yceD NIAGMN_24230 0.14 -0.6
4,739,870 + yceD NIAGMN_24230 0.46 -0.4
4,739,883 + yceD NIAGMN_24230 0.48 -0.6
4,739,883 + yceD NIAGMN_24230 0.48 +2.0
4,739,883 + yceD NIAGMN_24230 0.48 -1.5
4,739,892 + yceD NIAGMN_24230 0.50 -0.0
4,739,892 + yceD NIAGMN_24230 0.50 -1.0
4,739,892 + yceD NIAGMN_24230 0.50 +0.3
4,739,946 + yceD NIAGMN_24230 0.60 +0.0
4,739,946 + yceD NIAGMN_24230 0.60 -0.4
4,740,028 + yceD NIAGMN_24230 0.76 -1.9
4,740,127 + -1.4
4,740,139 + -0.4
4,740,146 + +2.0
4,740,150 + +2.0
4,740,150 + -0.6
4,740,150 + -0.7
4,740,185 + -1.4
4,740,345 + rpmF NIAGMN_24235 0.81 +1.0
4,740,348 + rpmF NIAGMN_24235 0.83 +2.0
4,740,371 + -0.9
4,740,371 + +0.4
4,740,375 + -0.4

Or see this region's nucleotide sequence