Strain Fitness in Escherichia coli ECRC102 around NIAGMN_22145

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmdtB and mdtA overlap by 1 nucleotidesmdtA and NIAGMN_22155 are separated by 279 nucleotidesNIAGMN_22155 and NIAGMN_22165 are separated by 271 nucleotidesNIAGMN_22165 and ibsA are separated by 271 nucleotides NIAGMN_22140: mdtB - multidrug efflux RND transporter permease subunit MdtB, at 4,366,554 to 4,369,676 mdtB NIAGMN_22145: mdtA - multidrug efflux RND transporter subunit MdtA, at 4,369,676 to 4,370,923 mdtA NIAGMN_22155: NIAGMN_22155 - Type I toxin-antitoxin system Ibs family toxin, at 4,371,203 to 4,371,259 _22155 NIAGMN_22165: NIAGMN_22165 - Type I toxin-antitoxin system Ibs family toxin, at 4,371,531 to 4,371,587 _22165 NIAGMN_22175: ibsA - Small toxic protein IbsA, at 4,371,859 to 4,371,918 ibsA Position (kb) 4369 4370 4371Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4368.771 kb on - strand, within mdtBat 4368.777 kb on - strand, within mdtBat 4368.864 kb on + strand, within mdtBat 4369.010 kb on + strand, within mdtBat 4369.181 kb on + strand, within mdtBat 4369.379 kb on + strandat 4369.626 kb on - strandat 4370.093 kb on + strand, within mdtAat 4370.111 kb on + strand, within mdtAat 4370.111 kb on + strand, within mdtAat 4370.386 kb on + strand, within mdtAat 4370.621 kb on + strand, within mdtAat 4370.659 kb on + strand, within mdtAat 4370.665 kb on + strand, within mdtAat 4370.668 kb on + strand, within mdtAat 4370.678 kb on - strand, within mdtAat 4370.880 kb on - strandat 4370.880 kb on - strandat 4370.909 kb on - strandat 4370.928 kb on - strandat 4370.966 kb on + strandat 4370.966 kb on + strandat 4370.967 kb on - strandat 4371.078 kb on + strandat 4371.187 kb on - strandat 4371.187 kb on - strandat 4371.190 kb on + strandat 4371.214 kb on - strand, within NIAGMN_22155at 4371.214 kb on - strand, within NIAGMN_22155at 4371.234 kb on + strand, within NIAGMN_22155at 4371.235 kb on - strand, within NIAGMN_22155at 4371.241 kb on + strand, within NIAGMN_22155at 4371.242 kb on - strand, within NIAGMN_22155at 4371.242 kb on - strand, within NIAGMN_22155at 4371.255 kb on - strandat 4371.255 kb on - strandat 4371.255 kb on - strandat 4371.258 kb on - strandat 4371.306 kb on + strandat 4371.408 kb on - strandat 4371.514 kb on + strandat 4371.562 kb on + strand, within NIAGMN_22165at 4371.562 kb on + strand, within NIAGMN_22165at 4371.563 kb on - strand, within NIAGMN_22165at 4371.568 kb on + strand, within NIAGMN_22165at 4371.569 kb on - strand, within NIAGMN_22165at 4371.583 kb on - strandat 4371.583 kb on - strandat 4371.586 kb on - strandat 4371.592 kb on - strandat 4371.888 kb on - strand, within ibsAat 4371.888 kb on - strand, within ibsAat 4371.894 kb on - strand, within ibsAat 4371.901 kb on - strand, within ibsAat 4371.916 kb on + strandat 4371.916 kb on + strandat 4371.916 kb on + strandat 4371.917 kb on - strandat 4371.917 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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4,368,771 - mdtB NIAGMN_22140 0.71 -0.0
4,368,777 - mdtB NIAGMN_22140 0.71 -0.3
4,368,864 + mdtB NIAGMN_22140 0.74 +0.7
4,369,010 + mdtB NIAGMN_22140 0.79 -0.8
4,369,181 + mdtB NIAGMN_22140 0.84 +1.6
4,369,379 + +0.8
4,369,626 - +0.2
4,370,093 + mdtA NIAGMN_22145 0.33 +0.7
4,370,111 + mdtA NIAGMN_22145 0.35 +2.3
4,370,111 + mdtA NIAGMN_22145 0.35 -1.7
4,370,386 + mdtA NIAGMN_22145 0.57 +0.7
4,370,621 + mdtA NIAGMN_22145 0.76 +2.0
4,370,659 + mdtA NIAGMN_22145 0.79 +1.0
4,370,665 + mdtA NIAGMN_22145 0.79 -0.3
4,370,668 + mdtA NIAGMN_22145 0.79 +2.0
4,370,678 - mdtA NIAGMN_22145 0.80 +0.2
4,370,880 - -0.2
4,370,880 - +0.1
4,370,909 - +3.0
4,370,928 - -0.0
4,370,966 + -0.0
4,370,966 + +0.7
4,370,967 - +0.4
4,371,078 + -0.6
4,371,187 - -0.6
4,371,187 - -1.8
4,371,190 + +0.3
4,371,214 - NIAGMN_22155 0.19 -1.1
4,371,214 - NIAGMN_22155 0.19 +1.0
4,371,234 + NIAGMN_22155 0.54 -1.5
4,371,235 - NIAGMN_22155 0.56 +1.2
4,371,241 + NIAGMN_22155 0.67 +0.4
4,371,242 - NIAGMN_22155 0.68 +0.4
4,371,242 - NIAGMN_22155 0.68 -0.1
4,371,255 - +1.2
4,371,255 - -1.0
4,371,255 - +2.0
4,371,258 - +2.0
4,371,306 + +1.2
4,371,408 - +2.0
4,371,514 + +0.1
4,371,562 + NIAGMN_22165 0.54 +1.0
4,371,562 + NIAGMN_22165 0.54 -0.8
4,371,563 - NIAGMN_22165 0.56 -0.9
4,371,568 + NIAGMN_22165 0.65 -0.5
4,371,569 - NIAGMN_22165 0.67 -0.4
4,371,583 - +0.2
4,371,583 - +0.4
4,371,586 - +0.8
4,371,592 - +1.0
4,371,888 - ibsA NIAGMN_22175 0.48 -0.7
4,371,888 - ibsA NIAGMN_22175 0.48 -0.0
4,371,894 - ibsA NIAGMN_22175 0.58 -1.0
4,371,901 - ibsA NIAGMN_22175 0.70 +0.4
4,371,916 + +1.0
4,371,916 + +0.2
4,371,916 + +2.0
4,371,917 - -0.8
4,371,917 - +1.4

Or see this region's nucleotide sequence