Strain Fitness in Escherichia coli ECRC102 around NIAGMN_20205

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntevgS and evgA are separated by 4 nucleotidesevgA and evgL overlap by 4 nucleotidesevgL and emrK are separated by 389 nucleotides NIAGMN_20200: evgS - acid-sensing system histidine kinase EvgS, at 3,985,568 to 3,989,161 evgS NIAGMN_20205: evgA - acid-sensing system DNA-binding response regulator EvgA, at 3,989,166 to 3,989,780 evgA NIAGMN_20210: evgL - Protein EvgL, at 3,989,777 to 3,989,806 evgL NIAGMN_20215: emrK - multidrug efflux MFS transporter periplasmic adaptor subunit EmrK, at 3,990,196 to 3,991,359 emrK Position (kb) 3989 3990Strain fitness (log2 ratio) -2 -1 0 1 2at 3988.769 kb on - strand, within evgSat 3989.247 kb on - strand, within evgAat 3989.249 kb on - strand, within evgAat 3989.319 kb on - strand, within evgAat 3989.319 kb on - strand, within evgAat 3989.417 kb on - strand, within evgAat 3989.457 kb on - strand, within evgAat 3989.552 kb on + strand, within evgAat 3989.648 kb on + strand, within evgAat 3989.903 kb on + strandat 3989.904 kb on - strandat 3990.289 kb on + strandat 3990.324 kb on + strand, within emrKat 3990.547 kb on + strand, within emrKat 3990.602 kb on - strand, within emrKat 3990.677 kb on + strand, within emrK

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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3,988,769 - evgS NIAGMN_20200 0.89 +0.3
3,989,247 - evgA NIAGMN_20205 0.13 +0.3
3,989,249 - evgA NIAGMN_20205 0.13 -0.3
3,989,319 - evgA NIAGMN_20205 0.25 +0.6
3,989,319 - evgA NIAGMN_20205 0.25 -2.7
3,989,417 - evgA NIAGMN_20205 0.41 -0.6
3,989,457 - evgA NIAGMN_20205 0.47 +1.9
3,989,552 + evgA NIAGMN_20205 0.63 -0.2
3,989,648 + evgA NIAGMN_20205 0.78 -0.9
3,989,903 + -0.9
3,989,904 - +0.5
3,990,289 + +0.9
3,990,324 + emrK NIAGMN_20215 0.11 -1.1
3,990,547 + emrK NIAGMN_20215 0.30 -0.8
3,990,602 - emrK NIAGMN_20215 0.35 +1.3
3,990,677 + emrK NIAGMN_20215 0.41 -1.0

Or see this region's nucleotide sequence