Strain Fitness in Escherichia coli ECRC102 around NIAGMN_20200

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_20190 and yfdE are separated by 32 nucleotidesyfdE and evgS are separated by 55 nucleotidesevgS and evgA are separated by 4 nucleotidesevgA and evgL overlap by 4 nucleotides NIAGMN_20190: NIAGMN_20190 - CoA-transferase, at 3,984,371 to 3,984,601 _20190 NIAGMN_20195: yfdE - CoA:oxalate CoA-transferase, at 3,984,634 to 3,985,512 yfdE NIAGMN_20200: evgS - acid-sensing system histidine kinase EvgS, at 3,985,568 to 3,989,161 evgS NIAGMN_20205: evgA - acid-sensing system DNA-binding response regulator EvgA, at 3,989,166 to 3,989,780 evgA NIAGMN_20210: evgL - Protein EvgL, at 3,989,777 to 3,989,806 evgL Position (kb) 3985 3986 3987 3988 3989 3990Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3984.654 kb on - strandat 3984.656 kb on + strandat 3984.678 kb on + strandat 3984.737 kb on + strand, within yfdEat 3984.737 kb on + strand, within yfdEat 3984.737 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.765 kb on + strand, within yfdEat 3984.870 kb on + strand, within yfdEat 3984.902 kb on - strand, within yfdEat 3984.971 kb on + strand, within yfdEat 3985.021 kb on + strand, within yfdEat 3985.022 kb on - strand, within yfdEat 3985.023 kb on + strand, within yfdEat 3985.023 kb on + strand, within yfdEat 3985.069 kb on + strand, within yfdEat 3985.295 kb on + strand, within yfdEat 3985.397 kb on + strand, within yfdEat 3985.510 kb on + strandat 3985.533 kb on + strandat 3985.774 kb on + strandat 3985.791 kb on - strandat 3985.865 kb on + strandat 3985.968 kb on - strand, within evgSat 3985.997 kb on + strand, within evgSat 3986.155 kb on - strand, within evgSat 3986.215 kb on - strand, within evgSat 3986.222 kb on + strand, within evgSat 3987.152 kb on + strand, within evgSat 3987.157 kb on + strand, within evgSat 3987.449 kb on + strand, within evgSat 3987.668 kb on - strand, within evgSat 3987.757 kb on + strand, within evgSat 3987.765 kb on + strand, within evgSat 3988.769 kb on - strand, within evgSat 3989.247 kb on - strand, within evgAat 3989.249 kb on - strand, within evgAat 3989.319 kb on - strand, within evgAat 3989.319 kb on - strand, within evgAat 3989.417 kb on - strand, within evgAat 3989.457 kb on - strand, within evgAat 3989.552 kb on + strand, within evgAat 3989.648 kb on + strand, within evgAat 3989.903 kb on + strandat 3989.904 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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3,984,654 - +0.3
3,984,656 + +0.9
3,984,678 + +1.5
3,984,737 + yfdE NIAGMN_20195 0.12 +0.3
3,984,737 + yfdE NIAGMN_20195 0.12 -0.1
3,984,737 + yfdE NIAGMN_20195 0.12 +1.0
3,984,739 + yfdE NIAGMN_20195 0.12 -0.7
3,984,739 + yfdE NIAGMN_20195 0.12 +1.1
3,984,739 + yfdE NIAGMN_20195 0.12 -1.3
3,984,739 + yfdE NIAGMN_20195 0.12 -0.1
3,984,739 + yfdE NIAGMN_20195 0.12 -0.6
3,984,765 + yfdE NIAGMN_20195 0.15 -0.1
3,984,870 + yfdE NIAGMN_20195 0.27 +0.1
3,984,902 - yfdE NIAGMN_20195 0.30 +0.1
3,984,971 + yfdE NIAGMN_20195 0.38 -0.4
3,985,021 + yfdE NIAGMN_20195 0.44 +2.3
3,985,022 - yfdE NIAGMN_20195 0.44 +0.9
3,985,023 + yfdE NIAGMN_20195 0.44 -0.1
3,985,023 + yfdE NIAGMN_20195 0.44 -0.7
3,985,069 + yfdE NIAGMN_20195 0.49 -0.1
3,985,295 + yfdE NIAGMN_20195 0.75 +0.3
3,985,397 + yfdE NIAGMN_20195 0.87 -0.1
3,985,510 + -1.4
3,985,533 + +1.4
3,985,774 + +2.9
3,985,791 - -0.1
3,985,865 + +0.3
3,985,968 - evgS NIAGMN_20200 0.11 +0.1
3,985,997 + evgS NIAGMN_20200 0.12 -1.5
3,986,155 - evgS NIAGMN_20200 0.16 -0.6
3,986,215 - evgS NIAGMN_20200 0.18 -1.3
3,986,222 + evgS NIAGMN_20200 0.18 +4.7
3,987,152 + evgS NIAGMN_20200 0.44 -0.5
3,987,157 + evgS NIAGMN_20200 0.44 +0.1
3,987,449 + evgS NIAGMN_20200 0.52 +1.9
3,987,668 - evgS NIAGMN_20200 0.58 -0.4
3,987,757 + evgS NIAGMN_20200 0.61 +1.9
3,987,765 + evgS NIAGMN_20200 0.61 +1.3
3,988,769 - evgS NIAGMN_20200 0.89 +0.3
3,989,247 - evgA NIAGMN_20205 0.13 +0.3
3,989,249 - evgA NIAGMN_20205 0.13 -0.3
3,989,319 - evgA NIAGMN_20205 0.25 +0.6
3,989,319 - evgA NIAGMN_20205 0.25 -2.7
3,989,417 - evgA NIAGMN_20205 0.41 -0.6
3,989,457 - evgA NIAGMN_20205 0.47 +1.9
3,989,552 + evgA NIAGMN_20205 0.63 -0.2
3,989,648 + evgA NIAGMN_20205 0.78 -0.9
3,989,903 + -0.9
3,989,904 - +0.5

Or see this region's nucleotide sequence