Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfF and hyfE are separated by 19 nucleotides hyfE and hyfD are separated by 11 nucleotides
NIAGMN_19645: hyfF - hydrogenase 4 subunit F, at 3,877,079 to 3,878,644
hyfF
NIAGMN_19650: hyfE - hydrogenase 4 membrane subunit, at 3,878,664 to 3,879,314
hyfE
NIAGMN_19655: hyfD - hydrogenase 4 subunit HyfD, at 3,879,326 to 3,880,765
hyfD
Position (kb)
3878
3879
3880 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3877.729 kb on + strand, within hyfF at 3877.730 kb on - strand, within hyfF at 3877.835 kb on - strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.842 kb on + strand, within hyfF at 3877.843 kb on - strand, within hyfF at 3878.009 kb on + strand, within hyfF at 3878.010 kb on - strand, within hyfF at 3878.144 kb on - strand, within hyfF at 3878.144 kb on - strand, within hyfF at 3878.149 kb on + strand, within hyfF at 3878.150 kb on - strand, within hyfF at 3878.158 kb on - strand, within hyfF at 3878.158 kb on - strand, within hyfF at 3878.158 kb on - strand, within hyfF at 3878.158 kb on - strand, within hyfF at 3878.193 kb on + strand, within hyfF at 3878.194 kb on - strand, within hyfF at 3878.194 kb on - strand, within hyfF at 3878.316 kb on + strand, within hyfF at 3878.370 kb on + strand, within hyfF at 3878.447 kb on - strand, within hyfF at 3878.542 kb on + strand at 3878.543 kb on - strand at 3878.546 kb on + strand at 3878.546 kb on + strand at 3878.547 kb on - strand at 3878.547 kb on - strand at 3878.547 kb on - strand at 3878.547 kb on - strand at 3878.547 kb on - strand at 3878.550 kb on - strand at 3878.652 kb on + strand at 3878.652 kb on + strand at 3878.653 kb on - strand at 3878.734 kb on - strand, within hyfE at 3878.851 kb on + strand, within hyfE at 3879.003 kb on + strand, within hyfE at 3879.004 kb on - strand, within hyfE at 3879.004 kb on - strand, within hyfE at 3879.082 kb on + strand, within hyfE at 3879.083 kb on - strand, within hyfE at 3879.083 kb on - strand, within hyfE at 3879.215 kb on - strand, within hyfE at 3879.294 kb on + strand at 3879.303 kb on - strand at 3879.303 kb on - strand at 3879.303 kb on - strand at 3879.303 kb on - strand at 3879.711 kb on + strand, within hyfD at 3879.712 kb on - strand, within hyfD at 3879.728 kb on + strand, within hyfD at 3879.729 kb on - strand, within hyfD at 3879.729 kb on - strand, within hyfD at 3879.729 kb on - strand, within hyfD at 3879.729 kb on - strand, within hyfD at 3879.772 kb on - strand, within hyfD at 3879.897 kb on + strand, within hyfD at 3879.898 kb on - strand, within hyfD at 3879.898 kb on - strand, within hyfD at 3879.898 kb on - strand, within hyfD at 3879.898 kb on - strand, within hyfD at 3880.003 kb on - strand, within hyfD at 3880.147 kb on - strand, within hyfD at 3880.147 kb on - strand, within hyfD at 3880.164 kb on - strand, within hyfD at 3880.228 kb on - strand, within hyfD at 3880.228 kb on - strand, within hyfD at 3880.269 kb on - strand, within hyfD at 3880.299 kb on + strand, within hyfD at 3880.299 kb on + strand, within hyfD at 3880.302 kb on + strand, within hyfD
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 3,877,729 + hyfF NIAGMN_19645 0.42 -0.7 3,877,730 - hyfF NIAGMN_19645 0.42 +0.2 3,877,835 - hyfF NIAGMN_19645 0.48 -1.2 3,877,842 + hyfF NIAGMN_19645 0.49 +0.0 3,877,842 + hyfF NIAGMN_19645 0.49 -1.9 3,877,842 + hyfF NIAGMN_19645 0.49 +0.8 3,877,842 + hyfF NIAGMN_19645 0.49 -1.8 3,877,842 + hyfF NIAGMN_19645 0.49 +1.8 3,877,843 - hyfF NIAGMN_19645 0.49 -1.0 3,878,009 + hyfF NIAGMN_19645 0.59 -0.2 3,878,010 - hyfF NIAGMN_19645 0.59 -0.5 3,878,144 - hyfF NIAGMN_19645 0.68 -0.5 3,878,144 - hyfF NIAGMN_19645 0.68 +1.7 3,878,149 + hyfF NIAGMN_19645 0.68 +0.8 3,878,150 - hyfF NIAGMN_19645 0.68 +2.8 3,878,158 - hyfF NIAGMN_19645 0.69 -0.2 3,878,158 - hyfF NIAGMN_19645 0.69 -0.3 3,878,158 - hyfF NIAGMN_19645 0.69 -0.2 3,878,158 - hyfF NIAGMN_19645 0.69 -0.6 3,878,193 + hyfF NIAGMN_19645 0.71 +0.8 3,878,194 - hyfF NIAGMN_19645 0.71 +1.5 3,878,194 - hyfF NIAGMN_19645 0.71 +0.2 3,878,316 + hyfF NIAGMN_19645 0.79 +0.8 3,878,370 + hyfF NIAGMN_19645 0.82 +0.2 3,878,447 - hyfF NIAGMN_19645 0.87 -1.9 3,878,542 + -0.2 3,878,543 - +0.8 3,878,546 + +1.2 3,878,546 + +0.4 3,878,547 - -0.2 3,878,547 - -1.2 3,878,547 - +0.3 3,878,547 - +0.7 3,878,547 - +0.8 3,878,550 - +0.8 3,878,652 + -1.6 3,878,652 + +1.2 3,878,653 - +0.8 3,878,734 - hyfE NIAGMN_19650 0.11 -1.5 3,878,851 + hyfE NIAGMN_19650 0.29 +0.8 3,879,003 + hyfE NIAGMN_19650 0.52 +0.2 3,879,004 - hyfE NIAGMN_19650 0.52 +1.1 3,879,004 - hyfE NIAGMN_19650 0.52 +1.6 3,879,082 + hyfE NIAGMN_19650 0.64 +0.8 3,879,083 - hyfE NIAGMN_19650 0.64 +0.4 3,879,083 - hyfE NIAGMN_19650 0.64 -1.0 3,879,215 - hyfE NIAGMN_19650 0.85 -0.3 3,879,294 + -0.2 3,879,303 - -0.2 3,879,303 - -1.9 3,879,303 - +0.5 3,879,303 - -1.1 3,879,711 + hyfD NIAGMN_19655 0.27 -0.3 3,879,712 - hyfD NIAGMN_19655 0.27 +0.2 3,879,728 + hyfD NIAGMN_19655 0.28 -1.4 3,879,729 - hyfD NIAGMN_19655 0.28 +0.5 3,879,729 - hyfD NIAGMN_19655 0.28 -0.5 3,879,729 - hyfD NIAGMN_19655 0.28 +1.2 3,879,729 - hyfD NIAGMN_19655 0.28 +0.4 3,879,772 - hyfD NIAGMN_19655 0.31 +0.2 3,879,897 + hyfD NIAGMN_19655 0.40 -0.2 3,879,898 - hyfD NIAGMN_19655 0.40 +0.0 3,879,898 - hyfD NIAGMN_19655 0.40 +0.4 3,879,898 - hyfD NIAGMN_19655 0.40 +1.6 3,879,898 - hyfD NIAGMN_19655 0.40 +0.2 3,880,003 - hyfD NIAGMN_19655 0.47 -1.0 3,880,147 - hyfD NIAGMN_19655 0.57 +1.8 3,880,147 - hyfD NIAGMN_19655 0.57 +0.0 3,880,164 - hyfD NIAGMN_19655 0.58 +0.2 3,880,228 - hyfD NIAGMN_19655 0.63 -0.8 3,880,228 - hyfD NIAGMN_19655 0.63 -0.5 3,880,269 - hyfD NIAGMN_19655 0.65 +0.9 3,880,299 + hyfD NIAGMN_19655 0.68 +0.6 3,880,299 + hyfD NIAGMN_19655 0.68 -1.2 3,880,302 + hyfD NIAGMN_19655 0.68 -0.8
Or see this region's nucleotide sequence