Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zapA and ygfB are separated by 167 nucleotides ygfB and pepP are separated by 25 nucleotides pepP and ubiH overlap by 4 nucleotides
NIAGMN_17260: zapA - cell division protein ZapA, at 3,408,660 to 3,408,989
zapA
NIAGMN_17265: ygfB - UPF0149 family protein YgfB, at 3,409,157 to 3,409,735
ygfB
NIAGMN_17270: pepP - Xaa-Pro aminopeptidase, at 3,409,761 to 3,411,086
pepP
NIAGMN_17275: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 3,411,083 to 3,412,261
ubiH
Position (kb)
3409
3410
3411
3412 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3408.803 kb on - strand, within zapA at 3408.843 kb on - strand, within zapA at 3408.843 kb on - strand, within zapA at 3408.843 kb on - strand, within zapA at 3408.843 kb on - strand, within zapA at 3408.855 kb on - strand, within zapA at 3408.855 kb on - strand, within zapA at 3408.855 kb on - strand, within zapA at 3409.057 kb on + strand at 3409.058 kb on - strand at 3409.058 kb on - strand at 3409.058 kb on - strand at 3409.058 kb on - strand at 3409.059 kb on + strand at 3409.059 kb on + strand at 3409.059 kb on + strand at 3409.059 kb on + strand at 3409.059 kb on + strand at 3409.059 kb on + strand at 3409.059 kb on + strand at 3409.060 kb on - strand at 3409.060 kb on - strand at 3409.060 kb on - strand at 3409.060 kb on - strand at 3409.060 kb on - strand at 3409.060 kb on - strand at 3409.063 kb on + strand at 3409.064 kb on - strand at 3409.064 kb on - strand at 3409.121 kb on + strand at 3409.121 kb on + strand at 3409.121 kb on + strand at 3409.121 kb on + strand at 3409.121 kb on + strand at 3409.121 kb on + strand at 3409.121 kb on + strand at 3409.122 kb on - strand at 3409.122 kb on - strand at 3409.122 kb on - strand at 3409.122 kb on - strand at 3409.122 kb on - strand at 3409.122 kb on - strand at 3409.131 kb on - strand at 3409.131 kb on - strand at 3409.131 kb on - strand at 3409.131 kb on - strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.156 kb on + strand at 3409.157 kb on - strand at 3409.157 kb on - strand at 3409.157 kb on - strand at 3409.157 kb on - strand at 3409.157 kb on - strand at 3409.157 kb on - strand at 3409.157 kb on - strand at 3409.157 kb on - strand at 3409.162 kb on + strand at 3409.162 kb on + strand at 3409.163 kb on - strand at 3409.163 kb on - strand at 3409.164 kb on + strand at 3409.164 kb on + strand at 3409.164 kb on + strand at 3409.165 kb on - strand at 3409.165 kb on - strand at 3409.165 kb on - strand at 3409.184 kb on + strand at 3409.184 kb on + strand at 3409.184 kb on + strand at 3409.184 kb on + strand at 3409.185 kb on - strand at 3409.202 kb on + strand at 3409.266 kb on + strand, within ygfB at 3409.266 kb on + strand, within ygfB at 3409.267 kb on - strand, within ygfB at 3409.267 kb on - strand, within ygfB at 3409.421 kb on + strand, within ygfB at 3409.421 kb on + strand, within ygfB at 3409.510 kb on + strand, within ygfB at 3409.510 kb on + strand, within ygfB at 3409.529 kb on - strand, within ygfB at 3409.529 kb on - strand, within ygfB at 3409.556 kb on - strand, within ygfB at 3409.571 kb on - strand, within ygfB at 3409.647 kb on - strand, within ygfB at 3409.668 kb on + strand, within ygfB at 3409.684 kb on + strand at 3409.685 kb on - strand at 3409.714 kb on - strand at 3409.728 kb on + strand at 3409.728 kb on + strand at 3409.729 kb on - strand at 3409.729 kb on - strand at 3409.729 kb on - strand at 3409.749 kb on - strand at 3409.760 kb on + strand at 3409.760 kb on + strand at 3409.861 kb on + strand at 3409.861 kb on + strand at 3409.884 kb on + strand at 3410.115 kb on + strand, within pepP at 3410.126 kb on + strand, within pepP at 3410.169 kb on + strand, within pepP at 3410.169 kb on + strand, within pepP at 3410.311 kb on + strand, within pepP at 3410.348 kb on + strand, within pepP at 3410.348 kb on + strand, within pepP at 3410.450 kb on + strand, within pepP at 3410.450 kb on + strand, within pepP at 3410.450 kb on + strand, within pepP at 3410.450 kb on + strand, within pepP at 3410.450 kb on + strand, within pepP at 3410.450 kb on + strand, within pepP at 3410.461 kb on + strand, within pepP at 3410.461 kb on + strand, within pepP at 3410.493 kb on + strand, within pepP at 3410.565 kb on + strand, within pepP at 3410.673 kb on + strand, within pepP at 3410.723 kb on + strand, within pepP at 3410.723 kb on + strand, within pepP at 3410.740 kb on + strand, within pepP at 3410.765 kb on + strand, within pepP at 3410.807 kb on + strand, within pepP at 3410.899 kb on + strand, within pepP at 3410.922 kb on + strand, within pepP at 3410.922 kb on + strand, within pepP at 3410.922 kb on + strand, within pepP at 3410.922 kb on + strand, within pepP at 3410.922 kb on + strand, within pepP at 3410.922 kb on + strand, within pepP at 3410.922 kb on + strand, within pepP at 3410.975 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 3,408,803 - zapA NIAGMN_17260 0.43 -1.2 3,408,843 - zapA NIAGMN_17260 0.55 -0.0 3,408,843 - zapA NIAGMN_17260 0.55 -0.2 3,408,843 - zapA NIAGMN_17260 0.55 -0.5 3,408,843 - zapA NIAGMN_17260 0.55 -0.1 3,408,855 - zapA NIAGMN_17260 0.59 -0.2 3,408,855 - zapA NIAGMN_17260 0.59 -0.0 3,408,855 - zapA NIAGMN_17260 0.59 -0.5 3,409,057 + -0.2 3,409,058 - +0.4 3,409,058 - +0.5 3,409,058 - -0.2 3,409,058 - +0.2 3,409,059 + -1.2 3,409,059 + -0.2 3,409,059 + -1.0 3,409,059 + -0.2 3,409,059 + -0.4 3,409,059 + -0.8 3,409,059 + -0.8 3,409,060 - +0.8 3,409,060 - -0.8 3,409,060 - -0.6 3,409,060 - -1.0 3,409,060 - +0.5 3,409,060 - -1.2 3,409,063 + -1.5 3,409,064 - +0.8 3,409,064 - -0.8 3,409,121 + +1.5 3,409,121 + -0.2 3,409,121 + +0.3 3,409,121 + +0.8 3,409,121 + -0.3 3,409,121 + -0.0 3,409,121 + -0.3 3,409,122 - +1.8 3,409,122 - +0.5 3,409,122 - +0.2 3,409,122 - +1.2 3,409,122 - -0.3 3,409,122 - -1.0 3,409,131 - -0.0 3,409,131 - +0.9 3,409,131 - +1.4 3,409,131 - +0.4 3,409,156 + -0.7 3,409,156 + +0.2 3,409,156 + -0.2 3,409,156 + +0.4 3,409,156 + +0.1 3,409,156 + -0.5 3,409,156 + -0.4 3,409,156 + -0.9 3,409,156 + -1.2 3,409,156 + -0.4 3,409,156 + +0.3 3,409,156 + -1.0 3,409,156 + -0.9 3,409,157 - -0.4 3,409,157 - -2.6 3,409,157 - +1.4 3,409,157 - -0.1 3,409,157 - +0.9 3,409,157 - +0.2 3,409,157 - +0.2 3,409,157 - -1.5 3,409,162 + -1.1 3,409,162 + -0.1 3,409,163 - +1.4 3,409,163 - +1.0 3,409,164 + +2.1 3,409,164 + -0.7 3,409,164 + -0.9 3,409,165 - -1.7 3,409,165 - +0.3 3,409,165 - +1.0 3,409,184 + -2.1 3,409,184 + +0.2 3,409,184 + +0.0 3,409,184 + -0.7 3,409,185 - -1.0 3,409,202 + -0.5 3,409,266 + ygfB NIAGMN_17265 0.19 -0.2 3,409,266 + ygfB NIAGMN_17265 0.19 +0.5 3,409,267 - ygfB NIAGMN_17265 0.19 -0.3 3,409,267 - ygfB NIAGMN_17265 0.19 -0.2 3,409,421 + ygfB NIAGMN_17265 0.46 -0.1 3,409,421 + ygfB NIAGMN_17265 0.46 +0.9 3,409,510 + ygfB NIAGMN_17265 0.61 +1.5 3,409,510 + ygfB NIAGMN_17265 0.61 +1.2 3,409,529 - ygfB NIAGMN_17265 0.64 -1.5 3,409,529 - ygfB NIAGMN_17265 0.64 +1.5 3,409,556 - ygfB NIAGMN_17265 0.69 +0.5 3,409,571 - ygfB NIAGMN_17265 0.72 -0.0 3,409,647 - ygfB NIAGMN_17265 0.85 +1.4 3,409,668 + ygfB NIAGMN_17265 0.88 -0.5 3,409,684 + -0.7 3,409,685 - -1.5 3,409,714 - -1.1 3,409,728 + +0.5 3,409,728 + -1.1 3,409,729 - +0.0 3,409,729 - -0.6 3,409,729 - +0.3 3,409,749 - +0.8 3,409,760 + -1.4 3,409,760 + -0.9 3,409,861 + -0.5 3,409,861 + -0.2 3,409,884 + -2.1 3,410,115 + pepP NIAGMN_17270 0.27 -1.2 3,410,126 + pepP NIAGMN_17270 0.28 +0.1 3,410,169 + pepP NIAGMN_17270 0.31 -0.0 3,410,169 + pepP NIAGMN_17270 0.31 +1.8 3,410,311 + pepP NIAGMN_17270 0.41 +0.9 3,410,348 + pepP NIAGMN_17270 0.44 +0.7 3,410,348 + pepP NIAGMN_17270 0.44 +1.8 3,410,450 + pepP NIAGMN_17270 0.52 -0.8 3,410,450 + pepP NIAGMN_17270 0.52 -0.2 3,410,450 + pepP NIAGMN_17270 0.52 +0.5 3,410,450 + pepP NIAGMN_17270 0.52 -0.5 3,410,450 + pepP NIAGMN_17270 0.52 -1.2 3,410,450 + pepP NIAGMN_17270 0.52 -0.5 3,410,461 + pepP NIAGMN_17270 0.53 -0.2 3,410,461 + pepP NIAGMN_17270 0.53 +1.6 3,410,493 + pepP NIAGMN_17270 0.55 -0.5 3,410,565 + pepP NIAGMN_17270 0.61 +1.0 3,410,673 + pepP NIAGMN_17270 0.69 -1.5 3,410,723 + pepP NIAGMN_17270 0.73 -0.5 3,410,723 + pepP NIAGMN_17270 0.73 -0.8 3,410,740 + pepP NIAGMN_17270 0.74 -1.2 3,410,765 + pepP NIAGMN_17270 0.76 +2.8 3,410,807 + pepP NIAGMN_17270 0.79 -0.5 3,410,899 + pepP NIAGMN_17270 0.86 -0.9 3,410,922 + pepP NIAGMN_17270 0.88 +0.4 3,410,922 + pepP NIAGMN_17270 0.88 +1.8 3,410,922 + pepP NIAGMN_17270 0.88 +1.3 3,410,922 + pepP NIAGMN_17270 0.88 +0.3 3,410,922 + pepP NIAGMN_17270 0.88 -1.0 3,410,922 + pepP NIAGMN_17270 0.88 -0.5 3,410,922 + pepP NIAGMN_17270 0.88 +0.8 3,410,975 + +0.4
Or see this region's nucleotide sequence