Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metC and exbB are separated by 251 nucleotides exbB and exbD are separated by 6 nucleotides exbD and yghA are separated by 271 nucleotides
NIAGMN_16725: metC - cystathionine beta-lyase, at 3,307,950 to 3,309,137
metC
NIAGMN_16730: exbB - tol-pal system-associated acyl-CoA thioesterase, at 3,309,389 to 3,310,123
exbB
NIAGMN_16735: exbD - TonB system transport protein ExbD, at 3,310,130 to 3,310,555
exbD
NIAGMN_16745: yghA - NADP(+)-dependent aldehyde reductase, at 3,310,827 to 3,311,711
yghA
Position (kb)
3310
3311 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3309.158 kb on - strand at 3309.185 kb on + strand at 3309.185 kb on + strand at 3309.186 kb on - strand at 3309.215 kb on + strand at 3309.215 kb on + strand at 3309.215 kb on + strand at 3309.216 kb on - strand at 3309.216 kb on - strand at 3309.290 kb on + strand at 3309.291 kb on - strand at 3309.344 kb on + strand at 3309.344 kb on + strand at 3309.347 kb on + strand at 3309.347 kb on + strand at 3309.347 kb on + strand at 3309.347 kb on + strand at 3309.347 kb on + strand at 3309.347 kb on + strand at 3309.348 kb on - strand at 3309.350 kb on + strand at 3309.351 kb on - strand at 3309.383 kb on + strand at 3309.383 kb on + strand at 3309.383 kb on + strand at 3309.397 kb on + strand at 3309.402 kb on + strand at 3309.402 kb on + strand at 3309.430 kb on + strand at 3309.430 kb on + strand at 3309.434 kb on + strand at 3309.434 kb on + strand at 3309.434 kb on + strand at 3309.434 kb on + strand at 3309.457 kb on + strand at 3309.517 kb on + strand, within exbB at 3309.518 kb on - strand, within exbB at 3309.525 kb on + strand, within exbB at 3309.525 kb on + strand, within exbB at 3309.525 kb on + strand, within exbB at 3309.525 kb on + strand, within exbB at 3309.554 kb on - strand, within exbB at 3309.636 kb on + strand, within exbB at 3309.637 kb on - strand, within exbB at 3309.706 kb on + strand, within exbB at 3309.706 kb on + strand, within exbB at 3309.710 kb on - strand, within exbB at 3309.718 kb on + strand, within exbB at 3309.719 kb on - strand, within exbB at 3309.753 kb on + strand, within exbB at 3309.754 kb on - strand, within exbB at 3309.782 kb on + strand, within exbB at 3309.924 kb on + strand, within exbB at 3309.924 kb on + strand, within exbB at 3309.971 kb on + strand, within exbB at 3309.971 kb on + strand, within exbB at 3309.971 kb on + strand, within exbB at 3309.971 kb on + strand, within exbB at 3309.971 kb on + strand, within exbB at 3309.971 kb on + strand, within exbB at 3309.971 kb on + strand, within exbB at 3309.971 kb on + strand, within exbB at 3309.972 kb on - strand, within exbB at 3309.972 kb on - strand, within exbB at 3309.972 kb on - strand, within exbB at 3310.035 kb on + strand, within exbB at 3310.035 kb on + strand, within exbB at 3310.035 kb on + strand, within exbB at 3310.036 kb on - strand, within exbB at 3310.144 kb on + strand at 3310.180 kb on + strand, within exbD at 3310.181 kb on - strand, within exbD at 3310.225 kb on + strand, within exbD at 3310.231 kb on + strand, within exbD at 3310.257 kb on + strand, within exbD at 3310.257 kb on + strand, within exbD at 3310.270 kb on + strand, within exbD at 3310.271 kb on - strand, within exbD at 3310.285 kb on + strand, within exbD at 3310.322 kb on + strand, within exbD at 3310.322 kb on + strand, within exbD at 3310.323 kb on - strand, within exbD at 3310.355 kb on - strand, within exbD at 3310.451 kb on - strand, within exbD at 3310.482 kb on + strand, within exbD at 3310.482 kb on + strand, within exbD at 3310.518 kb on + strand at 3310.518 kb on + strand at 3310.518 kb on + strand at 3310.553 kb on + strand at 3310.554 kb on - strand at 3310.576 kb on + strand at 3310.592 kb on - strand at 3310.695 kb on + strand at 3310.745 kb on - strand at 3310.775 kb on + strand at 3310.833 kb on + strand at 3310.834 kb on - strand at 3310.834 kb on - strand at 3310.876 kb on + strand at 3310.876 kb on + strand at 3310.877 kb on - strand at 3310.877 kb on - strand at 3310.900 kb on + strand at 3310.906 kb on + strand at 3310.906 kb on + strand at 3310.907 kb on - strand at 3310.907 kb on - strand at 3310.907 kb on - strand at 3310.907 kb on - strand at 3310.907 kb on - strand at 3310.907 kb on - strand at 3310.907 kb on - strand at 3310.907 kb on - strand at 3310.908 kb on + strand at 3310.908 kb on + strand at 3310.908 kb on + strand at 3310.908 kb on + strand at 3310.908 kb on + strand at 3310.908 kb on + strand at 3310.908 kb on + strand at 3310.908 kb on + strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3310.909 kb on - strand at 3311.037 kb on - strand, within yghA at 3311.037 kb on - strand, within yghA at 3311.037 kb on - strand, within yghA at 3311.049 kb on - strand, within yghA at 3311.049 kb on - strand, within yghA at 3311.049 kb on - strand, within yghA at 3311.086 kb on + strand, within yghA at 3311.087 kb on - strand, within yghA at 3311.087 kb on - strand, within yghA at 3311.109 kb on - strand, within yghA at 3311.116 kb on + strand, within yghA at 3311.116 kb on + strand, within yghA at 3311.116 kb on + strand, within yghA at 3311.117 kb on - strand, within yghA at 3311.117 kb on - strand, within yghA at 3311.117 kb on - strand, within yghA at 3311.124 kb on + strand, within yghA at 3311.125 kb on - strand, within yghA at 3311.171 kb on + strand, within yghA at 3311.171 kb on + strand, within yghA at 3311.171 kb on + strand, within yghA at 3311.171 kb on + strand, within yghA at 3311.171 kb on + strand, within yghA at 3311.171 kb on + strand, within yghA at 3311.171 kb on + strand, within yghA at 3311.171 kb on + strand, within yghA at 3311.172 kb on - strand, within yghA at 3311.172 kb on - strand, within yghA at 3311.172 kb on - strand, within yghA at 3311.172 kb on - strand, within yghA at 3311.279 kb on + strand, within yghA at 3311.318 kb on + strand, within yghA at 3311.319 kb on - strand, within yghA at 3311.334 kb on + strand, within yghA at 3311.335 kb on - strand, within yghA at 3311.335 kb on - strand, within yghA at 3311.506 kb on + strand, within yghA
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 3,309,158 - -0.3 3,309,185 + +0.3 3,309,185 + +0.8 3,309,186 - -0.4 3,309,215 + -0.2 3,309,215 + +0.7 3,309,215 + -1.6 3,309,216 - +0.2 3,309,216 - -1.5 3,309,290 + -0.2 3,309,291 - +2.8 3,309,344 + +0.8 3,309,344 + +0.8 3,309,347 + -0.2 3,309,347 + +0.1 3,309,347 + -0.6 3,309,347 + -0.4 3,309,347 + -2.0 3,309,347 + +0.6 3,309,348 - +1.4 3,309,350 + -0.0 3,309,351 - +1.8 3,309,383 + -1.0 3,309,383 + -1.4 3,309,383 + -3.1 3,309,397 + -0.2 3,309,402 + -2.6 3,309,402 + +1.8 3,309,430 + +0.2 3,309,430 + +0.3 3,309,434 + -1.1 3,309,434 + +0.2 3,309,434 + -1.8 3,309,434 + +0.8 3,309,457 + -1.2 3,309,517 + exbB NIAGMN_16730 0.17 -1.6 3,309,518 - exbB NIAGMN_16730 0.18 +1.4 3,309,525 + exbB NIAGMN_16730 0.19 -0.0 3,309,525 + exbB NIAGMN_16730 0.19 -0.7 3,309,525 + exbB NIAGMN_16730 0.19 -1.4 3,309,525 + exbB NIAGMN_16730 0.19 +0.2 3,309,554 - exbB NIAGMN_16730 0.22 +0.2 3,309,636 + exbB NIAGMN_16730 0.34 -0.7 3,309,637 - exbB NIAGMN_16730 0.34 +1.8 3,309,706 + exbB NIAGMN_16730 0.43 -0.4 3,309,706 + exbB NIAGMN_16730 0.43 -1.6 3,309,710 - exbB NIAGMN_16730 0.44 -0.6 3,309,718 + exbB NIAGMN_16730 0.45 +2.2 3,309,719 - exbB NIAGMN_16730 0.45 +0.2 3,309,753 + exbB NIAGMN_16730 0.50 -0.8 3,309,754 - exbB NIAGMN_16730 0.50 -0.6 3,309,782 + exbB NIAGMN_16730 0.53 -0.8 3,309,924 + exbB NIAGMN_16730 0.73 -2.0 3,309,924 + exbB NIAGMN_16730 0.73 +1.1 3,309,971 + exbB NIAGMN_16730 0.79 -0.8 3,309,971 + exbB NIAGMN_16730 0.79 +2.2 3,309,971 + exbB NIAGMN_16730 0.79 +1.2 3,309,971 + exbB NIAGMN_16730 0.79 -0.3 3,309,971 + exbB NIAGMN_16730 0.79 +0.5 3,309,971 + exbB NIAGMN_16730 0.79 -1.3 3,309,971 + exbB NIAGMN_16730 0.79 -0.1 3,309,971 + exbB NIAGMN_16730 0.79 +0.2 3,309,972 - exbB NIAGMN_16730 0.79 +0.8 3,309,972 - exbB NIAGMN_16730 0.79 +1.8 3,309,972 - exbB NIAGMN_16730 0.79 +0.8 3,310,035 + exbB NIAGMN_16730 0.88 +0.6 3,310,035 + exbB NIAGMN_16730 0.88 +1.8 3,310,035 + exbB NIAGMN_16730 0.88 -0.8 3,310,036 - exbB NIAGMN_16730 0.88 +0.8 3,310,144 + -0.6 3,310,180 + exbD NIAGMN_16735 0.12 -0.8 3,310,181 - exbD NIAGMN_16735 0.12 +0.8 3,310,225 + exbD NIAGMN_16735 0.22 -0.2 3,310,231 + exbD NIAGMN_16735 0.24 -1.0 3,310,257 + exbD NIAGMN_16735 0.30 -1.3 3,310,257 + exbD NIAGMN_16735 0.30 -0.6 3,310,270 + exbD NIAGMN_16735 0.33 +0.6 3,310,271 - exbD NIAGMN_16735 0.33 -0.8 3,310,285 + exbD NIAGMN_16735 0.36 +0.4 3,310,322 + exbD NIAGMN_16735 0.45 -1.2 3,310,322 + exbD NIAGMN_16735 0.45 -0.0 3,310,323 - exbD NIAGMN_16735 0.45 -2.5 3,310,355 - exbD NIAGMN_16735 0.53 -1.6 3,310,451 - exbD NIAGMN_16735 0.75 +0.5 3,310,482 + exbD NIAGMN_16735 0.83 +1.8 3,310,482 + exbD NIAGMN_16735 0.83 +0.9 3,310,518 + -1.0 3,310,518 + +0.2 3,310,518 + -2.0 3,310,553 + -1.7 3,310,554 - -0.4 3,310,576 + -0.2 3,310,592 - -1.1 3,310,695 + +1.0 3,310,745 - -1.2 3,310,775 + -1.8 3,310,833 + -2.8 3,310,834 - -1.1 3,310,834 - +1.1 3,310,876 + +0.2 3,310,876 + -2.8 3,310,877 - -1.4 3,310,877 - +0.4 3,310,900 + +1.3 3,310,906 + +0.7 3,310,906 + +0.4 3,310,907 - -0.0 3,310,907 - +1.8 3,310,907 - -0.2 3,310,907 - -0.9 3,310,907 - -0.6 3,310,907 - +0.0 3,310,907 - -0.5 3,310,907 - -1.7 3,310,908 + +0.8 3,310,908 + -0.4 3,310,908 + -0.6 3,310,908 + -2.1 3,310,908 + -1.1 3,310,908 + -1.0 3,310,908 + +0.5 3,310,908 + -0.6 3,310,909 - -2.0 3,310,909 - -0.9 3,310,909 - -1.0 3,310,909 - -0.0 3,310,909 - +0.8 3,310,909 - -0.2 3,310,909 - -1.1 3,310,909 - -1.2 3,310,909 - -3.1 3,310,909 - -0.6 3,310,909 - -0.6 3,311,037 - yghA NIAGMN_16745 0.24 -1.4 3,311,037 - yghA NIAGMN_16745 0.24 -0.6 3,311,037 - yghA NIAGMN_16745 0.24 -0.7 3,311,049 - yghA NIAGMN_16745 0.25 +0.8 3,311,049 - yghA NIAGMN_16745 0.25 -0.6 3,311,049 - yghA NIAGMN_16745 0.25 -0.6 3,311,086 + yghA NIAGMN_16745 0.29 -0.0 3,311,087 - yghA NIAGMN_16745 0.29 +0.5 3,311,087 - yghA NIAGMN_16745 0.29 +0.5 3,311,109 - yghA NIAGMN_16745 0.32 -0.2 3,311,116 + yghA NIAGMN_16745 0.33 -0.7 3,311,116 + yghA NIAGMN_16745 0.33 +0.5 3,311,116 + yghA NIAGMN_16745 0.33 -1.4 3,311,117 - yghA NIAGMN_16745 0.33 +0.2 3,311,117 - yghA NIAGMN_16745 0.33 +0.3 3,311,117 - yghA NIAGMN_16745 0.33 +0.1 3,311,124 + yghA NIAGMN_16745 0.34 -0.5 3,311,125 - yghA NIAGMN_16745 0.34 -0.7 3,311,171 + yghA NIAGMN_16745 0.39 -0.8 3,311,171 + yghA NIAGMN_16745 0.39 +0.4 3,311,171 + yghA NIAGMN_16745 0.39 +1.6 3,311,171 + yghA NIAGMN_16745 0.39 -0.5 3,311,171 + yghA NIAGMN_16745 0.39 -1.0 3,311,171 + yghA NIAGMN_16745 0.39 -0.0 3,311,171 + yghA NIAGMN_16745 0.39 +0.2 3,311,171 + yghA NIAGMN_16745 0.39 -2.2 3,311,172 - yghA NIAGMN_16745 0.39 +0.2 3,311,172 - yghA NIAGMN_16745 0.39 +0.0 3,311,172 - yghA NIAGMN_16745 0.39 +1.5 3,311,172 - yghA NIAGMN_16745 0.39 -0.4 3,311,279 + yghA NIAGMN_16745 0.51 +2.0 3,311,318 + yghA NIAGMN_16745 0.55 -1.1 3,311,319 - yghA NIAGMN_16745 0.56 +0.8 3,311,334 + yghA NIAGMN_16745 0.57 +0.2 3,311,335 - yghA NIAGMN_16745 0.57 +0.9 3,311,335 - yghA NIAGMN_16745 0.57 +1.1 3,311,506 + yghA NIAGMN_16745 0.77 +1.5
Or see this region's nucleotide sequence