Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafV and NIAGMN_07655 are separated by 117 nucleotides NIAGMN_07655 and NIAGMN_07660 are separated by 180 nucleotides NIAGMN_07660 and NIAGMN_07665 are separated by 47 nucleotides NIAGMN_07665 and rhsA overlap by 17 nucleotides
NIAGMN_07650: yafV - 2-oxoglutaramate amidase, at 1,463,787 to 1,464,557
yafV
NIAGMN_07655: NIAGMN_07655 - ISAs1-like element ISEc26 family transposase, at 1,464,675 to 1,465,847
_07655
NIAGMN_07660: NIAGMN_07660 - dCTP deaminase, at 1,466,028 to 1,466,291
_07660
NIAGMN_07665: NIAGMN_07665 - YdcD, at 1,466,339 to 1,466,509
_07665
NIAGMN_07670: rhsA - RHS repeat protein, at 1,466,493 to 1,468,253
rhsA
Position (kb)
1464
1465
1466 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1463.685 kb on - strand at 1463.693 kb on + strand at 1463.740 kb on + strand at 1463.748 kb on + strand at 1463.757 kb on - strand at 1463.781 kb on + strand at 1463.781 kb on + strand at 1463.781 kb on + strand at 1463.782 kb on - strand at 1463.782 kb on - strand at 1463.879 kb on + strand, within yafV at 1463.879 kb on + strand, within yafV at 1463.879 kb on + strand, within yafV at 1463.880 kb on - strand, within yafV at 1463.880 kb on - strand, within yafV at 1463.883 kb on - strand, within yafV at 1463.971 kb on + strand, within yafV at 1463.971 kb on + strand, within yafV at 1463.971 kb on + strand, within yafV at 1463.971 kb on + strand, within yafV at 1463.972 kb on - strand, within yafV at 1464.105 kb on - strand, within yafV at 1464.144 kb on + strand, within yafV at 1464.144 kb on + strand, within yafV at 1464.146 kb on + strand, within yafV at 1464.146 kb on + strand, within yafV at 1464.147 kb on - strand, within yafV at 1464.191 kb on + strand, within yafV at 1464.191 kb on + strand, within yafV at 1464.210 kb on + strand, within yafV at 1464.217 kb on + strand, within yafV at 1464.218 kb on - strand, within yafV at 1464.218 kb on - strand, within yafV at 1464.218 kb on - strand, within yafV at 1464.267 kb on + strand, within yafV at 1464.267 kb on + strand, within yafV at 1464.267 kb on + strand, within yafV at 1464.268 kb on - strand, within yafV at 1464.268 kb on - strand, within yafV at 1464.345 kb on + strand, within yafV at 1464.346 kb on - strand, within yafV at 1464.346 kb on - strand, within yafV at 1464.346 kb on - strand, within yafV at 1464.346 kb on - strand, within yafV at 1464.346 kb on - strand, within yafV at 1464.505 kb on - strand at 1464.507 kb on - strand at 1464.507 kb on - strand at 1464.568 kb on + strand at 1464.568 kb on + strand at 1464.569 kb on - strand at 1464.752 kb on - strand at 1464.752 kb on - strand at 1464.856 kb on - strand, within NIAGMN_07655 at 1464.906 kb on + strand, within NIAGMN_07655 at 1464.957 kb on - strand, within NIAGMN_07655 at 1464.999 kb on - strand, within NIAGMN_07655 at 1465.015 kb on - strand, within NIAGMN_07655 at 1465.032 kb on - strand, within NIAGMN_07655 at 1465.277 kb on - strand, within NIAGMN_07655 at 1465.746 kb on + strand at 1465.801 kb on - strand at 1465.902 kb on + strand at 1465.934 kb on + strand at 1465.996 kb on - strand at 1465.996 kb on - strand at 1466.184 kb on - strand, within NIAGMN_07660 at 1466.371 kb on - strand, within NIAGMN_07665 at 1466.371 kb on - strand, within NIAGMN_07665 at 1466.584 kb on + strand at 1466.722 kb on - strand, within rhsA at 1466.733 kb on + strand, within rhsA at 1466.773 kb on + strand, within rhsA at 1466.779 kb on + strand, within rhsA at 1466.822 kb on + strand, within rhsA at 1466.823 kb on - strand, within rhsA
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 1,463,685 - +1.1 1,463,693 + +0.6 1,463,740 + -1.3 1,463,748 + -0.5 1,463,757 - -1.1 1,463,781 + -0.2 1,463,781 + +0.6 1,463,781 + +1.6 1,463,782 - -0.5 1,463,782 - -0.0 1,463,879 + yafV NIAGMN_07650 0.12 +1.4 1,463,879 + yafV NIAGMN_07650 0.12 -2.3 1,463,879 + yafV NIAGMN_07650 0.12 -0.1 1,463,880 - yafV NIAGMN_07650 0.12 -0.8 1,463,880 - yafV NIAGMN_07650 0.12 +1.3 1,463,883 - yafV NIAGMN_07650 0.12 -0.8 1,463,971 + yafV NIAGMN_07650 0.24 +0.6 1,463,971 + yafV NIAGMN_07650 0.24 -1.4 1,463,971 + yafV NIAGMN_07650 0.24 +0.0 1,463,971 + yafV NIAGMN_07650 0.24 -1.0 1,463,972 - yafV NIAGMN_07650 0.24 +3.8 1,464,105 - yafV NIAGMN_07650 0.41 -0.8 1,464,144 + yafV NIAGMN_07650 0.46 -2.3 1,464,144 + yafV NIAGMN_07650 0.46 +0.5 1,464,146 + yafV NIAGMN_07650 0.47 +0.6 1,464,146 + yafV NIAGMN_07650 0.47 -0.0 1,464,147 - yafV NIAGMN_07650 0.47 +1.6 1,464,191 + yafV NIAGMN_07650 0.52 -1.0 1,464,191 + yafV NIAGMN_07650 0.52 +0.3 1,464,210 + yafV NIAGMN_07650 0.55 -0.9 1,464,217 + yafV NIAGMN_07650 0.56 +0.2 1,464,218 - yafV NIAGMN_07650 0.56 +0.8 1,464,218 - yafV NIAGMN_07650 0.56 +0.1 1,464,218 - yafV NIAGMN_07650 0.56 -0.7 1,464,267 + yafV NIAGMN_07650 0.62 +0.0 1,464,267 + yafV NIAGMN_07650 0.62 -1.4 1,464,267 + yafV NIAGMN_07650 0.62 -0.5 1,464,268 - yafV NIAGMN_07650 0.62 -1.8 1,464,268 - yafV NIAGMN_07650 0.62 -0.2 1,464,345 + yafV NIAGMN_07650 0.72 +1.1 1,464,346 - yafV NIAGMN_07650 0.73 +1.1 1,464,346 - yafV NIAGMN_07650 0.73 +1.0 1,464,346 - yafV NIAGMN_07650 0.73 +1.1 1,464,346 - yafV NIAGMN_07650 0.73 +0.8 1,464,346 - yafV NIAGMN_07650 0.73 +1.5 1,464,505 - +0.1 1,464,507 - -2.3 1,464,507 - -1.2 1,464,568 + +0.8 1,464,568 + -0.5 1,464,569 - -0.2 1,464,752 - -0.8 1,464,752 - -1.0 1,464,856 - NIAGMN_07655 0.15 -0.2 1,464,906 + NIAGMN_07655 0.20 -0.5 1,464,957 - NIAGMN_07655 0.24 -0.5 1,464,999 - NIAGMN_07655 0.28 +0.5 1,465,015 - NIAGMN_07655 0.29 +0.4 1,465,032 - NIAGMN_07655 0.30 +0.7 1,465,277 - NIAGMN_07655 0.51 +0.2 1,465,746 + +1.8 1,465,801 - +0.5 1,465,902 + +0.6 1,465,934 + +0.3 1,465,996 - -0.4 1,465,996 - -0.4 1,466,184 - NIAGMN_07660 0.59 +0.1 1,466,371 - NIAGMN_07665 0.19 -0.7 1,466,371 - NIAGMN_07665 0.19 -1.4 1,466,584 + +2.2 1,466,722 - rhsA NIAGMN_07670 0.13 +0.3 1,466,733 + rhsA NIAGMN_07670 0.14 -0.5 1,466,773 + rhsA NIAGMN_07670 0.16 +0.8 1,466,779 + rhsA NIAGMN_07670 0.16 +0.2 1,466,822 + rhsA NIAGMN_07670 0.19 -0.8 1,466,823 - rhsA NIAGMN_07670 0.19 +0.8
Or see this region's nucleotide sequence