Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadA and Lys_trna are separated by 277 nucleotides Lys_trna and Lys_trna are separated by 146 nucleotides Lys_trna and Val_trna are separated by 3 nucleotides Val_trna and Lys_trna are separated by 51 nucleotides Lys_trna and Val_trna are separated by 2 nucleotides Val_trna and Lys_trna are separated by 35 nucleotides Lys_trna and cpoB are separated by 164 nucleotides
NIAGMN_04810: nadA - quinolinate synthase NadA, at 874,048 to 875,091
nadA
NIAGMN_04815: Lys_trna - tRNA-Lys(ttt), at 875,369 to 875,444
_trna
NIAGMN_04820: Lys_trna - tRNA-Lys(ttt), at 875,591 to 875,666
_trna
NIAGMN_04825: Val_trna - tRNA-Val(tac), at 875,670 to 875,745
_trna
NIAGMN_04830: Lys_trna - tRNA-Lys(ttt), at 875,797 to 875,872
_trna
NIAGMN_04835: Val_trna - tRNA-Val(tac), at 875,875 to 875,950
_trna
NIAGMN_04840: Lys_trna - tRNA-Lys(ttt), at 875,986 to 876,061
_trna
NIAGMN_04845: cpoB - cell division protein CpoB, at 876,226 to 877,017
cpoB
Position (kb)
875
876 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 874.396 kb on - strand, within nadA at 874.510 kb on + strand, within nadA at 874.841 kb on - strand, within nadA at 874.899 kb on + strand, within nadA at 875.220 kb on + strand at 875.264 kb on + strand at 875.293 kb on - strand at 875.335 kb on - strand at 875.352 kb on - strand at 875.365 kb on - strand at 875.471 kb on - strand at 875.567 kb on - strand at 875.567 kb on - strand at 875.749 kb on - strand at 875.761 kb on - strand at 875.761 kb on - strand at 875.764 kb on - strand at 875.771 kb on - strand at 875.771 kb on - strand at 875.791 kb on - strand at 875.791 kb on - strand at 875.795 kb on - strand at 875.795 kb on - strand at 875.957 kb on - strand at 875.957 kb on - strand at 875.957 kb on - strand at 875.969 kb on - strand at 875.969 kb on - strand at 875.969 kb on - strand at 875.975 kb on - strand at 875.975 kb on - strand at 875.975 kb on - strand at 875.975 kb on - strand at 875.979 kb on - strand at 875.979 kb on - strand at 875.979 kb on - strand at 875.979 kb on - strand at 876.155 kb on - strand at 876.155 kb on - strand at 876.172 kb on - strand at 876.221 kb on - strand at 876.221 kb on - strand at 876.221 kb on - strand at 876.227 kb on + strand at 876.228 kb on - strand at 876.228 kb on - strand at 876.228 kb on - strand at 876.228 kb on - strand at 876.228 kb on - strand at 876.270 kb on + strand at 876.271 kb on - strand at 876.271 kb on - strand at 876.279 kb on - strand at 876.279 kb on - strand at 876.390 kb on - strand, within cpoB at 876.390 kb on - strand, within cpoB at 876.414 kb on - strand, within cpoB at 876.414 kb on - strand, within cpoB
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 874,396 - nadA NIAGMN_04810 0.33 +0.6 874,510 + nadA NIAGMN_04810 0.44 -0.2 874,841 - nadA NIAGMN_04810 0.76 -0.3 874,899 + nadA NIAGMN_04810 0.82 -0.3 875,220 + +2.0 875,264 + -0.6 875,293 - +1.2 875,335 - +0.1 875,352 - -0.1 875,365 - -1.2 875,471 - -0.0 875,567 - +0.2 875,567 - +1.6 875,749 - -3.7 875,761 - -0.7 875,761 - -0.9 875,764 - -0.7 875,771 - +1.2 875,771 - -1.4 875,791 - +0.5 875,791 - -1.0 875,795 - -0.4 875,795 - +0.1 875,957 - -0.1 875,957 - +0.4 875,957 - -2.9 875,969 - -2.2 875,969 - -0.0 875,969 - -1.9 875,975 - -1.2 875,975 - -0.4 875,975 - -1.8 875,975 - -0.9 875,979 - -0.7 875,979 - +0.2 875,979 - -0.0 875,979 - +0.4 876,155 - +0.1 876,155 - +1.4 876,172 - +2.0 876,221 - -0.2 876,221 - +0.0 876,221 - -0.6 876,227 + +1.0 876,228 - -1.0 876,228 - -0.0 876,228 - +0.1 876,228 - +0.8 876,228 - +0.4 876,270 + +0.4 876,271 - -0.7 876,271 - -0.9 876,279 - +0.7 876,279 - +1.5 876,390 - cpoB NIAGMN_04845 0.21 -0.2 876,390 - cpoB NIAGMN_04845 0.21 +1.3 876,414 - cpoB NIAGMN_04845 0.24 -0.5 876,414 - cpoB NIAGMN_04845 0.24 -0.1
Or see this region's nucleotide sequence