Strain Fitness in Escherichia coli ECRC102 around NIAGMN_04035

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnfsA and NIAGMN_04025 overlap by 17 nucleotidesNIAGMN_04025 and grxA are separated by 159 nucleotidesgrxA and ybjM are separated by 29 nucleotidesybjM and ybjL are separated by 269 nucleotides NIAGMN_04020: nfsA - nitroreductase NfsA, at 723,847 to 724,569 nfsA NIAGMN_04025: NIAGMN_04025 - DUF1418 domain-containing protein, at 724,553 to 724,840 _04025 NIAGMN_04030: grxA - glutaredoxin 1, at 725,000 to 725,257 grxA NIAGMN_04035: ybjM - Inner membrane protein YbjM, at 725,287 to 725,664 ybjM NIAGMN_04040: ybjL - aspartate:alanine antiporter, at 725,934 to 727,619 ybjL Position (kb) 725 726Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 724.309 kb on + strand, within nfsAat 724.477 kb on - strand, within nfsAat 724.548 kb on + strandat 724.549 kb on - strandat 724.570 kb on + strandat 724.596 kb on + strand, within NIAGMN_04025at 724.652 kb on - strand, within NIAGMN_04025at 724.826 kb on - strandat 724.826 kb on - strandat 724.826 kb on - strandat 724.840 kb on + strandat 724.887 kb on + strandat 724.887 kb on + strandat 724.888 kb on - strandat 724.888 kb on - strandat 724.888 kb on - strandat 724.978 kb on + strandat 724.978 kb on + strandat 724.978 kb on + strandat 725.036 kb on + strand, within grxAat 725.036 kb on + strand, within grxAat 725.096 kb on + strand, within grxAat 725.102 kb on + strand, within grxAat 725.102 kb on + strand, within grxAat 725.102 kb on + strand, within grxAat 725.106 kb on + strand, within grxAat 725.106 kb on + strand, within grxAat 725.106 kb on + strand, within grxAat 725.106 kb on + strand, within grxAat 725.110 kb on + strand, within grxAat 725.131 kb on + strand, within grxAat 725.142 kb on + strand, within grxAat 725.142 kb on + strand, within grxAat 725.166 kb on + strand, within grxAat 725.203 kb on + strand, within grxAat 725.329 kb on - strand, within ybjMat 725.375 kb on - strand, within ybjMat 725.377 kb on + strand, within ybjMat 725.378 kb on - strand, within ybjMat 725.433 kb on + strand, within ybjMat 725.433 kb on + strand, within ybjMat 725.434 kb on - strand, within ybjMat 725.434 kb on - strand, within ybjMat 725.687 kb on + strandat 725.749 kb on + strandat 725.755 kb on - strandat 725.755 kb on - strandat 725.772 kb on - strandat 725.774 kb on - strandat 725.774 kb on - strandat 725.810 kb on - strandat 725.811 kb on + strandat 725.812 kb on - strandat 725.823 kb on - strandat 726.082 kb on + strandat 726.211 kb on + strand, within ybjLat 726.211 kb on + strand, within ybjLat 726.262 kb on + strand, within ybjLat 726.262 kb on + strand, within ybjLat 726.263 kb on - strand, within ybjLat 726.266 kb on + strand, within ybjLat 726.307 kb on + strand, within ybjLat 726.318 kb on - strand, within ybjLat 726.432 kb on + strand, within ybjLat 726.436 kb on + strand, within ybjLat 726.436 kb on + strand, within ybjLat 726.436 kb on + strand, within ybjL

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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724,309 + nfsA NIAGMN_04020 0.64 -1.3
724,477 - nfsA NIAGMN_04020 0.87 +0.1
724,548 + -1.0
724,549 - +0.2
724,570 + +0.4
724,596 + NIAGMN_04025 0.15 -2.1
724,652 - NIAGMN_04025 0.34 -0.2
724,826 - +0.7
724,826 - +0.4
724,826 - +1.0
724,840 + +0.9
724,887 + +1.0
724,887 + -2.5
724,888 - -0.0
724,888 - -0.2
724,888 - +1.0
724,978 + +0.2
724,978 + -0.2
724,978 + -0.5
725,036 + grxA NIAGMN_04030 0.14 +0.4
725,036 + grxA NIAGMN_04030 0.14 -3.0
725,096 + grxA NIAGMN_04030 0.37 -0.7
725,102 + grxA NIAGMN_04030 0.40 -0.3
725,102 + grxA NIAGMN_04030 0.40 +0.4
725,102 + grxA NIAGMN_04030 0.40 -2.1
725,106 + grxA NIAGMN_04030 0.41 -2.5
725,106 + grxA NIAGMN_04030 0.41 -1.6
725,106 + grxA NIAGMN_04030 0.41 +0.4
725,106 + grxA NIAGMN_04030 0.41 +0.9
725,110 + grxA NIAGMN_04030 0.43 -0.8
725,131 + grxA NIAGMN_04030 0.51 +1.0
725,142 + grxA NIAGMN_04030 0.55 -0.3
725,142 + grxA NIAGMN_04030 0.55 -2.0
725,166 + grxA NIAGMN_04030 0.64 -0.6
725,203 + grxA NIAGMN_04030 0.79 +0.2
725,329 - ybjM NIAGMN_04035 0.11 -0.0
725,375 - ybjM NIAGMN_04035 0.23 -0.9
725,377 + ybjM NIAGMN_04035 0.24 -0.7
725,378 - ybjM NIAGMN_04035 0.24 +0.9
725,433 + ybjM NIAGMN_04035 0.39 +0.8
725,433 + ybjM NIAGMN_04035 0.39 +3.0
725,434 - ybjM NIAGMN_04035 0.39 +0.7
725,434 - ybjM NIAGMN_04035 0.39 +2.0
725,687 + -0.3
725,749 + -0.0
725,755 - -0.3
725,755 - -0.3
725,772 - +0.2
725,774 - +0.4
725,774 - -1.7
725,810 - -0.3
725,811 + +2.0
725,812 - +0.3
725,823 - +2.0
726,082 + +1.0
726,211 + ybjL NIAGMN_04040 0.16 +1.0
726,211 + ybjL NIAGMN_04040 0.16 +0.1
726,262 + ybjL NIAGMN_04040 0.19 -0.4
726,262 + ybjL NIAGMN_04040 0.19 +0.7
726,263 - ybjL NIAGMN_04040 0.20 -0.1
726,266 + ybjL NIAGMN_04040 0.20 +0.1
726,307 + ybjL NIAGMN_04040 0.22 -0.6
726,318 - ybjL NIAGMN_04040 0.23 -0.2
726,432 + ybjL NIAGMN_04040 0.30 -0.1
726,436 + ybjL NIAGMN_04040 0.30 -2.7
726,436 + ybjL NIAGMN_04040 0.30 +3.0
726,436 + ybjL NIAGMN_04040 0.30 -0.0

Or see this region's nucleotide sequence