Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_03330 and NIAGMN_03335 are separated by 293 nucleotides NIAGMN_03335 and NIAGMN_03340 overlap by 4 nucleotides NIAGMN_03340 and NIAGMN_03345 are separated by 46 nucleotides NIAGMN_03345 and NIAGMN_03350 are separated by 49 nucleotides NIAGMN_03350 and NIAGMN_03355 overlap by 1 nucleotides NIAGMN_03355 and yeeS are separated by 62 nucleotides
NIAGMN_03330: NIAGMN_03330 - restriction endonuclease subunit M, at 597,981 to 598,823
_03330
NIAGMN_03335: NIAGMN_03335 - DUF5983 domain-containing protein, at 599,117 to 599,608
_03335
NIAGMN_03340: NIAGMN_03340 - toxin, at 599,605 to 599,979
_03340
NIAGMN_03345: NIAGMN_03345 - antitoxin, at 600,026 to 600,400
_03345
NIAGMN_03350: NIAGMN_03350 - Phage-related protein, at 600,450 to 600,563
_03350
NIAGMN_03355: NIAGMN_03355 - DUF987 domain-containing protein, at 600,563 to 600,784
_03355
NIAGMN_03360: yeeS - UPF0758 protein YeeS, at 600,847 to 601,323
yeeS
Position (kb) 599
600 Strain fitness (log2 ratio) -2
-1
0
1
2 at 598.653 kb on - strand, within NIAGMN_03330 at 598.724 kb on + strand, within NIAGMN_03330 at 598.847 kb on - strand at 598.847 kb on - strand at 599.713 kb on + strand, within NIAGMN_03340 at 599.953 kb on + strand at 599.953 kb on + strand at 599.982 kb on + strand at 599.982 kb on + strand at 600.223 kb on - strand, within NIAGMN_03345 at 600.223 kb on - strand, within NIAGMN_03345 at 600.273 kb on + strand, within NIAGMN_03345 at 600.426 kb on + strand at 600.427 kb on - strand at 600.437 kb on + strand at 600.649 kb on + strand, within NIAGMN_03355 at 600.650 kb on - strand, within NIAGMN_03355 at 600.670 kb on + strand, within NIAGMN_03355 at 600.671 kb on - strand, within NIAGMN_03355 at 600.672 kb on + strand, within NIAGMN_03355 at 600.704 kb on - strand, within NIAGMN_03355 at 600.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 598,653 - NIAGMN_03330 0.80 +0.4 598,724 + NIAGMN_03330 0.88 -0.6 598,847 - -0.3 598,847 - +1.0 599,713 + NIAGMN_03340 0.29 +1.3 599,953 + -0.3 599,953 + +0.5 599,982 + -1.6 599,982 + +1.0 600,223 - NIAGMN_03345 0.53 -0.6 600,223 - NIAGMN_03345 0.53 +0.3 600,273 + NIAGMN_03345 0.66 +1.2 600,426 + -0.6 600,427 - +1.0 600,437 + +1.7 600,649 + NIAGMN_03355 0.39 -0.3 600,650 - NIAGMN_03355 0.39 +0.7 600,670 + NIAGMN_03355 0.48 +0.0 600,671 - NIAGMN_03355 0.49 -2.0 600,672 + NIAGMN_03355 0.49 +2.0 600,704 - NIAGMN_03355 0.64 -1.0 600,812 - +0.0
Or see this region's nucleotide sequence