Strain Fitness in Escherichia coli ECRC102 around NIAGMN_03265

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdmsC and dmsB are separated by 1 nucleotidesdmsB and dmsA are separated by 10 nucleotidesdmsA and serS are separated by 238 nucleotides NIAGMN_03255: dmsC - dimethyl sulfoxide reductase anchor subunit DmsC, at 578,784 to 579,647 dmsC NIAGMN_03260: dmsB - dimethylsulfoxide reductase subunit B, at 579,649 to 580,266 dmsB NIAGMN_03265: dmsA - dimethylsulfoxide reductase subunit A, at 580,277 to 582,721 dmsA NIAGMN_03275: serS - serine--tRNA ligase, at 582,960 to 584,252 serS Position (kb) 580 581 582 583Strain fitness (log2 ratio) -1 0 1 2 3at 579.496 kb on - strand, within dmsCat 579.901 kb on - strand, within dmsBat 580.082 kb on + strand, within dmsBat 580.149 kb on - strand, within dmsBat 580.306 kb on + strandat 580.306 kb on + strandat 580.307 kb on - strandat 580.631 kb on - strand, within dmsAat 580.828 kb on + strand, within dmsAat 580.831 kb on - strand, within dmsAat 581.157 kb on + strand, within dmsAat 581.448 kb on + strand, within dmsAat 581.486 kb on - strand, within dmsAat 581.594 kb on - strand, within dmsAat 581.703 kb on + strand, within dmsAat 582.078 kb on + strand, within dmsAat 582.223 kb on - strand, within dmsAat 582.500 kb on - strandat 582.500 kb on - strandat 582.532 kb on + strandat 582.869 kb on + strandat 582.869 kb on + strandat 582.886 kb on + strandat 582.886 kb on + strandat 582.887 kb on - strandat 582.959 kb on - strandat 582.971 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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579,496 - dmsC NIAGMN_03255 0.82 -0.8
579,901 - dmsB NIAGMN_03260 0.41 +1.6
580,082 + dmsB NIAGMN_03260 0.70 +0.5
580,149 - dmsB NIAGMN_03260 0.81 -0.1
580,306 + -1.3
580,306 + -1.1
580,307 - +0.6
580,631 - dmsA NIAGMN_03265 0.14 +1.0
580,828 + dmsA NIAGMN_03265 0.23 +0.2
580,831 - dmsA NIAGMN_03265 0.23 +3.0
581,157 + dmsA NIAGMN_03265 0.36 +1.3
581,448 + dmsA NIAGMN_03265 0.48 +0.5
581,486 - dmsA NIAGMN_03265 0.49 +1.4
581,594 - dmsA NIAGMN_03265 0.54 +2.0
581,703 + dmsA NIAGMN_03265 0.58 -0.1
582,078 + dmsA NIAGMN_03265 0.74 +0.9
582,223 - dmsA NIAGMN_03265 0.80 -1.6
582,500 - -1.0
582,500 - -0.3
582,532 + +1.0
582,869 + +1.0
582,869 + +1.0
582,886 + +0.0
582,886 + +0.4
582,887 - +0.6
582,959 - -0.3
582,971 - +2.0

Or see this region's nucleotide sequence