Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheA and motB are separated by 4 nucleotides motB and motA overlap by 4 nucleotides motA and flhC are separated by 126 nucleotides flhC and flhD are separated by 2 nucleotides
NIAGMN_01940: cheA - chemotaxis protein CheA, at 350,870 to 352,834
cheA
NIAGMN_01945: motB - flagellar motor protein MotB, at 352,839 to 353,765
motB
NIAGMN_01950: motA - flagellar motor stator protein MotA, at 353,762 to 354,649
motA
NIAGMN_01955: flhC - flagellar transcriptional regulator FlhC, at 354,776 to 355,354
flhC
NIAGMN_01960: flhD - flagellar transcriptional regulator FlhD, at 355,357 to 355,707
flhD
Position (kb) 353
354
355 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 352.821 kb on - strand at 352.821 kb on - strand at 353.196 kb on - strand, within motB at 354.250 kb on + strand, within motA at 354.424 kb on + strand, within motA at 354.426 kb on + strand, within motA at 354.565 kb on - strand at 354.631 kb on - strand at 354.636 kb on - strand at 354.636 kb on - strand at 354.644 kb on - strand at 354.644 kb on - strand at 354.821 kb on - strand at 354.844 kb on + strand, within flhC at 354.963 kb on - strand, within flhC at 355.051 kb on - strand, within flhC at 355.087 kb on + strand, within flhC at 355.232 kb on + strand, within flhC at 355.396 kb on - strand, within flhD at 355.483 kb on - strand, within flhD at 355.594 kb on + strand, within flhD
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 352,821 - +1.0 352,821 - -0.0 353,196 - motB NIAGMN_01945 0.39 -0.8 354,250 + motA NIAGMN_01950 0.55 +3.0 354,424 + motA NIAGMN_01950 0.75 -1.0 354,426 + motA NIAGMN_01950 0.75 -1.1 354,565 - +1.0 354,631 - +2.0 354,636 - +4.5 354,636 - -0.0 354,644 - -1.9 354,644 - +2.0 354,821 - +0.6 354,844 + flhC NIAGMN_01955 0.12 +0.6 354,963 - flhC NIAGMN_01955 0.32 +1.2 355,051 - flhC NIAGMN_01955 0.47 +0.4 355,087 + flhC NIAGMN_01955 0.54 -0.5 355,232 + flhC NIAGMN_01955 0.79 -0.4 355,396 - flhD NIAGMN_01960 0.11 +2.0 355,483 - flhD NIAGMN_01960 0.36 +0.2 355,594 + flhD NIAGMN_01960 0.68 +0.5
Or see this region's nucleotide sequence