Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00485

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmalI and NIAGMN_00485 are separated by 174 nucleotidesNIAGMN_00485 and ydgJ are separated by 35 nucleotides NIAGMN_00480: malI - mal regulon transcriptional regulator MalI, at 76,059 to 77,087 malI NIAGMN_00485: NIAGMN_00485 - hypothetical protein, at 77,262 to 78,434 _00485 NIAGMN_00490: ydgJ - Uncharacterized oxidoreductase YdgJ, at 78,470 to 79,510 ydgJ Position (kb) 77 78 79Strain fitness (log2 ratio) -1 0 1 2 3at 76.624 kb on + strand, within malIat 77.363 kb on + strandat 77.378 kb on + strandat 77.660 kb on + strand, within NIAGMN_00485at 78.303 kb on + strand, within NIAGMN_00485at 78.603 kb on + strand, within ydgJat 78.604 kb on - strand, within ydgJat 78.604 kb on - strand, within ydgJat 78.604 kb on - strand, within ydgJat 78.678 kb on - strand, within ydgJat 78.763 kb on - strand, within ydgJat 78.797 kb on - strand, within ydgJat 78.799 kb on - strand, within ydgJat 78.799 kb on - strand, within ydgJat 78.920 kb on + strand, within ydgJat 78.948 kb on - strand, within ydgJ

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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76,624 + malI NIAGMN_00480 0.55 -1.5
77,363 + +2.9
77,378 + -0.4
77,660 + NIAGMN_00485 0.34 +0.6
78,303 + NIAGMN_00485 0.89 +0.6
78,603 + ydgJ NIAGMN_00490 0.13 -0.7
78,604 - ydgJ NIAGMN_00490 0.13 +2.2
78,604 - ydgJ NIAGMN_00490 0.13 -0.1
78,604 - ydgJ NIAGMN_00490 0.13 -0.1
78,678 - ydgJ NIAGMN_00490 0.20 -0.1
78,763 - ydgJ NIAGMN_00490 0.28 +0.6
78,797 - ydgJ NIAGMN_00490 0.31 -1.5
78,799 - ydgJ NIAGMN_00490 0.32 -0.1
78,799 - ydgJ NIAGMN_00490 0.32 +0.4
78,920 + ydgJ NIAGMN_00490 0.43 -0.5
78,948 - ydgJ NIAGMN_00490 0.46 +0.5

Or see this region's nucleotide sequence