Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_04424

Experiment: vio MOI1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_04423 and OHPLBJKB_04424 are separated by 805 nucleotidesOHPLBJKB_04424 and OHPLBJKB_04425 overlap by 4 nucleotidesOHPLBJKB_04425 and OHPLBJKB_04426 are separated by 46 nucleotides OHPLBJKB_04423: OHPLBJKB_04423 - Plasmid-derived single-stranded DNA-binding protein, at 74,925 to 75,464 _04423 OHPLBJKB_04424: OHPLBJKB_04424 - hypothetical protein, at 76,270 to 76,461 _04424 OHPLBJKB_04425: OHPLBJKB_04425 - hypothetical protein, at 76,458 to 76,880 _04425 OHPLBJKB_04426: OHPLBJKB_04426 - Antirestriction protein KlcA, at 76,927 to 77,352 _04426 Position (kb) 76 77Strain fitness (log2 ratio) -2 -1 0 1 2at 75.397 kb on + strand, within OHPLBJKB_04423at 75.398 kb on - strand, within OHPLBJKB_04423at 75.398 kb on - strand, within OHPLBJKB_04423at 75.429 kb on + strandat 75.697 kb on + strandat 76.268 kb on + strandat 76.269 kb on - strandat 76.298 kb on + strand, within OHPLBJKB_04424at 76.298 kb on + strand, within OHPLBJKB_04424at 76.298 kb on + strand, within OHPLBJKB_04424at 76.299 kb on - strand, within OHPLBJKB_04424at 76.299 kb on - strand, within OHPLBJKB_04424at 76.299 kb on - strand, within OHPLBJKB_04424at 76.421 kb on + strand, within OHPLBJKB_04424at 76.455 kb on + strandat 76.489 kb on - strandat 76.564 kb on + strand, within OHPLBJKB_04425at 76.565 kb on - strand, within OHPLBJKB_04425at 76.702 kb on + strand, within OHPLBJKB_04425at 76.838 kb on + strandat 76.845 kb on + strandat 76.845 kb on + strandat 76.846 kb on - strandat 76.871 kb on + strandat 76.872 kb on - strandat 76.872 kb on - strandat 76.872 kb on - strandat 76.872 kb on - strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.876 kb on + strandat 76.877 kb on - strandat 76.877 kb on - strandat 76.877 kb on - strandat 76.877 kb on - strandat 76.877 kb on - strandat 76.877 kb on - strandat 76.877 kb on - strandat 76.877 kb on - strandat 76.877 kb on - strandat 76.877 kb on - strandat 77.023 kb on + strand, within OHPLBJKB_04426at 77.023 kb on + strand, within OHPLBJKB_04426at 77.023 kb on + strand, within OHPLBJKB_04426at 77.023 kb on + strand, within OHPLBJKB_04426at 77.023 kb on + strand, within OHPLBJKB_04426at 77.024 kb on - strand, within OHPLBJKB_04426at 77.024 kb on - strand, within OHPLBJKB_04426at 77.024 kb on - strand, within OHPLBJKB_04426at 77.042 kb on + strand, within OHPLBJKB_04426at 77.168 kb on + strand, within OHPLBJKB_04426at 77.169 kb on - strand, within OHPLBJKB_04426at 77.247 kb on - strand, within OHPLBJKB_04426at 77.252 kb on + strand, within OHPLBJKB_04426at 77.252 kb on + strand, within OHPLBJKB_04426at 77.252 kb on + strand, within OHPLBJKB_04426at 77.252 kb on + strand, within OHPLBJKB_04426at 77.253 kb on - strand, within OHPLBJKB_04426at 77.253 kb on - strand, within OHPLBJKB_04426at 77.253 kb on - strand, within OHPLBJKB_04426at 77.253 kb on - strand, within OHPLBJKB_04426at 77.288 kb on + strand, within OHPLBJKB_04426at 77.289 kb on - strand, within OHPLBJKB_04426at 77.289 kb on - strand, within OHPLBJKB_04426at 77.289 kb on - strand, within OHPLBJKB_04426at 77.345 kb on + strandat 77.377 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction vio MOI1
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75,397 + OHPLBJKB_04423 0.87 +1.0
75,398 - OHPLBJKB_04423 0.88 +0.9
75,398 - OHPLBJKB_04423 0.88 +0.7
75,429 + -1.2
75,697 + -0.8
76,268 + +2.0
76,269 - +0.4
76,298 + OHPLBJKB_04424 0.15 +0.3
76,298 + OHPLBJKB_04424 0.15 +1.7
76,298 + OHPLBJKB_04424 0.15 +1.7
76,299 - OHPLBJKB_04424 0.15 +0.3
76,299 - OHPLBJKB_04424 0.15 -0.3
76,299 - OHPLBJKB_04424 0.15 +0.0
76,421 + OHPLBJKB_04424 0.79 +0.9
76,455 + +0.4
76,489 - -0.6
76,564 + OHPLBJKB_04425 0.25 -0.9
76,565 - OHPLBJKB_04425 0.25 -0.3
76,702 + OHPLBJKB_04425 0.58 -0.1
76,838 + -0.5
76,845 + +0.1
76,845 + +0.5
76,846 - +1.3
76,871 + +0.9
76,872 - -0.3
76,872 - +0.7
76,872 - -0.3
76,872 - +0.2
76,876 + -1.6
76,876 + -1.5
76,876 + +1.4
76,876 + -0.1
76,876 + +0.1
76,876 + -0.2
76,876 + +1.7
76,876 + +0.7
76,876 + +1.7
76,876 + -0.6
76,876 + -0.8
76,876 + +0.4
76,876 + +1.0
76,876 + -0.7
76,876 + -0.5
76,877 - -0.4
76,877 - +1.7
76,877 - -1.8
76,877 - -0.5
76,877 - -0.3
76,877 - -1.6
76,877 - -0.0
76,877 - -0.3
76,877 - +0.1
76,877 - +1.7
77,023 + OHPLBJKB_04426 0.23 -1.1
77,023 + OHPLBJKB_04426 0.23 +1.1
77,023 + OHPLBJKB_04426 0.23 +0.5
77,023 + OHPLBJKB_04426 0.23 -0.2
77,023 + OHPLBJKB_04426 0.23 +0.9
77,024 - OHPLBJKB_04426 0.23 +1.7
77,024 - OHPLBJKB_04426 0.23 -0.2
77,024 - OHPLBJKB_04426 0.23 +1.7
77,042 + OHPLBJKB_04426 0.27 +0.1
77,168 + OHPLBJKB_04426 0.57 +0.3
77,169 - OHPLBJKB_04426 0.57 +0.1
77,247 - OHPLBJKB_04426 0.75 +0.2
77,252 + OHPLBJKB_04426 0.76 -2.4
77,252 + OHPLBJKB_04426 0.76 -0.9
77,252 + OHPLBJKB_04426 0.76 +0.5
77,252 + OHPLBJKB_04426 0.76 -1.1
77,253 - OHPLBJKB_04426 0.77 -1.1
77,253 - OHPLBJKB_04426 0.77 -0.6
77,253 - OHPLBJKB_04426 0.77 -0.2
77,253 - OHPLBJKB_04426 0.77 +1.1
77,288 + OHPLBJKB_04426 0.85 -1.1
77,289 - OHPLBJKB_04426 0.85 +0.6
77,289 - OHPLBJKB_04426 0.85 +0.6
77,289 - OHPLBJKB_04426 0.85 +0.4
77,345 + +0.7
77,377 - +1.0

Or see this region's nucleotide sequence