Strain Fitness in Pseudomonas sp. SVBP6 around COO64_RS21470

Experiment: supernatant control, M9 with 0.5X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCOO64_RS21465 and lpdA are separated by 2 nucleotideslpdA and COO64_RS21475 are separated by 259 nucleotides COO64_RS21465: COO64_RS21465 - dihydrolipoamide acetyltransferase family protein, at 57,762 to 59,033 _RS21465 COO64_RS21470: lpdA - dihydrolipoyl dehydrogenase, at 59,036 to 60,421 lpdA COO64_RS21475: COO64_RS21475 - hypothetical protein, at 60,681 to 61,259 _RS21475 Position (kb) 59 60 61Strain fitness (log2 ratio) -2 -1 0 1at 58.224 kb on + strand, within COO64_RS21465at 58.224 kb on + strand, within COO64_RS21465at 58.224 kb on + strand, within COO64_RS21465at 58.224 kb on + strand, within COO64_RS21465at 58.225 kb on - strand, within COO64_RS21465at 58.225 kb on - strand, within COO64_RS21465at 58.225 kb on - strand, within COO64_RS21465at 58.225 kb on - strand, within COO64_RS21465at 58.225 kb on - strand, within COO64_RS21465at 58.225 kb on - strand, within COO64_RS21465at 58.225 kb on - strand, within COO64_RS21465at 58.225 kb on - strand, within COO64_RS21465at 58.228 kb on + strand, within COO64_RS21465at 58.228 kb on + strand, within COO64_RS21465at 58.228 kb on + strand, within COO64_RS21465at 58.228 kb on + strand, within COO64_RS21465at 58.228 kb on + strand, within COO64_RS21465at 58.228 kb on + strand, within COO64_RS21465at 58.228 kb on + strand, within COO64_RS21465at 58.228 kb on + strand, within COO64_RS21465at 58.228 kb on + strand, within COO64_RS21465at 58.229 kb on - strand, within COO64_RS21465at 58.229 kb on - strand, within COO64_RS21465at 58.229 kb on - strand, within COO64_RS21465at 58.229 kb on - strand, within COO64_RS21465at 58.425 kb on + strand, within COO64_RS21465at 58.425 kb on + strand, within COO64_RS21465at 58.425 kb on + strand, within COO64_RS21465at 58.426 kb on - strand, within COO64_RS21465at 58.426 kb on - strand, within COO64_RS21465at 58.578 kb on + strand, within COO64_RS21465at 58.579 kb on - strand, within COO64_RS21465at 58.579 kb on - strand, within COO64_RS21465at 58.579 kb on - strand, within COO64_RS21465at 58.818 kb on - strand, within COO64_RS21465at 59.056 kb on + strandat 59.057 kb on - strandat 59.090 kb on + strandat 59.091 kb on - strandat 59.091 kb on - strandat 59.407 kb on + strand, within lpdAat 59.407 kb on + strand, within lpdAat 59.408 kb on - strand, within lpdAat 59.435 kb on - strand, within lpdAat 59.435 kb on - strand, within lpdAat 59.451 kb on + strand, within lpdAat 59.451 kb on + strand, within lpdAat 59.451 kb on + strand, within lpdAat 59.451 kb on + strand, within lpdAat 59.451 kb on + strand, within lpdAat 59.451 kb on + strand, within lpdAat 59.451 kb on + strand, within lpdAat 59.451 kb on + strand, within lpdAat 59.452 kb on - strand, within lpdAat 59.452 kb on - strand, within lpdAat 59.452 kb on - strand, within lpdAat 59.452 kb on - strand, within lpdAat 59.452 kb on - strand, within lpdAat 59.580 kb on - strand, within lpdAat 59.666 kb on + strand, within lpdAat 59.666 kb on + strand, within lpdAat 59.667 kb on - strand, within lpdAat 59.667 kb on - strand, within lpdAat 59.727 kb on - strand, within lpdAat 59.778 kb on + strand, within lpdAat 59.778 kb on + strand, within lpdAat 59.779 kb on - strand, within lpdAat 59.812 kb on + strand, within lpdAat 59.823 kb on + strand, within lpdAat 59.823 kb on + strand, within lpdAat 59.824 kb on - strand, within lpdAat 60.344 kb on - strandat 60.419 kb on + strandat 60.573 kb on + strandat 60.574 kb on - strandat 60.574 kb on - strandat 60.597 kb on + strandat 60.686 kb on + strandat 60.686 kb on + strandat 60.686 kb on + strandat 60.686 kb on + strandat 60.686 kb on + strandat 60.686 kb on + strandat 60.687 kb on - strandat 60.687 kb on - strandat 60.687 kb on - strandat 60.975 kb on + strand, within COO64_RS21475at 60.975 kb on + strand, within COO64_RS21475at 60.975 kb on + strand, within COO64_RS21475at 60.975 kb on + strand, within COO64_RS21475at 60.975 kb on + strand, within COO64_RS21475at 60.976 kb on - strand, within COO64_RS21475at 60.976 kb on - strand, within COO64_RS21475at 61.339 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.5X LB
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58,224 + COO64_RS21465 0.36 +0.8
58,224 + COO64_RS21465 0.36 -0.7
58,224 + COO64_RS21465 0.36 +0.1
58,224 + COO64_RS21465 0.36 +0.3
58,225 - COO64_RS21465 0.36 -0.6
58,225 - COO64_RS21465 0.36 -1.4
58,225 - COO64_RS21465 0.36 -0.6
58,225 - COO64_RS21465 0.36 -0.1
58,225 - COO64_RS21465 0.36 -0.2
58,225 - COO64_RS21465 0.36 +0.4
58,225 - COO64_RS21465 0.36 +0.5
58,225 - COO64_RS21465 0.36 -0.4
58,228 + COO64_RS21465 0.37 -0.2
58,228 + COO64_RS21465 0.37 +1.2
58,228 + COO64_RS21465 0.37 -0.2
58,228 + COO64_RS21465 0.37 -0.3
58,228 + COO64_RS21465 0.37 +0.8
58,228 + COO64_RS21465 0.37 -0.3
58,228 + COO64_RS21465 0.37 +0.1
58,228 + COO64_RS21465 0.37 -0.5
58,228 + COO64_RS21465 0.37 -0.1
58,229 - COO64_RS21465 0.37 +0.2
58,229 - COO64_RS21465 0.37 +0.2
58,229 - COO64_RS21465 0.37 +0.6
58,229 - COO64_RS21465 0.37 -0.7
58,425 + COO64_RS21465 0.52 -0.8
58,425 + COO64_RS21465 0.52 -0.1
58,425 + COO64_RS21465 0.52 +0.6
58,426 - COO64_RS21465 0.52 -0.3
58,426 - COO64_RS21465 0.52 +0.5
58,578 + COO64_RS21465 0.64 -0.3
58,579 - COO64_RS21465 0.64 -0.2
58,579 - COO64_RS21465 0.64 +0.5
58,579 - COO64_RS21465 0.64 -0.6
58,818 - COO64_RS21465 0.83 -0.4
59,056 + -0.3
59,057 - +0.1
59,090 + -0.4
59,091 - +0.2
59,091 - +1.5
59,407 + lpdA COO64_RS21470 0.27 +0.3
59,407 + lpdA COO64_RS21470 0.27 -0.7
59,408 - lpdA COO64_RS21470 0.27 -0.0
59,435 - lpdA COO64_RS21470 0.29 +0.4
59,435 - lpdA COO64_RS21470 0.29 +0.7
59,451 + lpdA COO64_RS21470 0.30 +0.5
59,451 + lpdA COO64_RS21470 0.30 -0.2
59,451 + lpdA COO64_RS21470 0.30 -1.6
59,451 + lpdA COO64_RS21470 0.30 -0.2
59,451 + lpdA COO64_RS21470 0.30 +0.4
59,451 + lpdA COO64_RS21470 0.30 -0.6
59,451 + lpdA COO64_RS21470 0.30 +0.7
59,451 + lpdA COO64_RS21470 0.30 -0.7
59,452 - lpdA COO64_RS21470 0.30 +0.7
59,452 - lpdA COO64_RS21470 0.30 +0.1
59,452 - lpdA COO64_RS21470 0.30 -0.6
59,452 - lpdA COO64_RS21470 0.30 -0.8
59,452 - lpdA COO64_RS21470 0.30 -0.6
59,580 - lpdA COO64_RS21470 0.39 -1.3
59,666 + lpdA COO64_RS21470 0.45 +0.3
59,666 + lpdA COO64_RS21470 0.45 +0.5
59,667 - lpdA COO64_RS21470 0.46 -0.2
59,667 - lpdA COO64_RS21470 0.46 -1.9
59,727 - lpdA COO64_RS21470 0.50 -0.6
59,778 + lpdA COO64_RS21470 0.54 -1.0
59,778 + lpdA COO64_RS21470 0.54 -0.8
59,779 - lpdA COO64_RS21470 0.54 +0.6
59,812 + lpdA COO64_RS21470 0.56 -0.7
59,823 + lpdA COO64_RS21470 0.57 -0.6
59,823 + lpdA COO64_RS21470 0.57 +1.0
59,824 - lpdA COO64_RS21470 0.57 +0.2
60,344 - -0.0
60,419 + -0.2
60,573 + +0.7
60,574 - +0.2
60,574 - +0.1
60,597 + -0.2
60,686 + -1.9
60,686 + +0.4
60,686 + +0.5
60,686 + +0.1
60,686 + -0.3
60,686 + +0.4
60,687 - -0.5
60,687 - -0.9
60,687 - -1.1
60,975 + COO64_RS21475 0.51 -0.8
60,975 + COO64_RS21475 0.51 -1.0
60,975 + COO64_RS21475 0.51 -0.1
60,975 + COO64_RS21475 0.51 +0.2
60,975 + COO64_RS21475 0.51 -0.1
60,976 - COO64_RS21475 0.51 -0.3
60,976 - COO64_RS21475 0.51 -0.3
61,339 - +0.7

Or see this region's nucleotide sequence