Experiment: supernatant control, M9 with 0.5X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS07700 and COO64_RS07705 overlap by 25 nucleotides COO64_RS07705 and imuA are separated by 6 nucleotides imuA and lexA overlap by 1 nucleotides
COO64_RS07700: COO64_RS07700 - error-prone DNA polymerase, at 29,859 to 32,963
_RS07700
COO64_RS07705: COO64_RS07705 - DNA polymerase Y family protein, at 32,939 to 34,354
_RS07705
COO64_RS07710: imuA - translesion DNA synthesis-associated protein ImuA, at 34,361 to 34,978
imuA
COO64_RS07715: lexA - transcriptional repressor LexA, at 34,978 to 35,595
lexA
Position (kb) 32
33
34
35 Strain fitness (log2 ratio) -2
-1
0
1 at 32.008 kb on + strand, within COO64_RS07700 at 32.009 kb on - strand, within COO64_RS07700 at 32.009 kb on - strand, within COO64_RS07700 at 32.009 kb on - strand, within COO64_RS07700 at 32.017 kb on + strand, within COO64_RS07700 at 32.018 kb on - strand, within COO64_RS07700 at 32.018 kb on - strand, within COO64_RS07700 at 32.018 kb on - strand, within COO64_RS07700 at 32.623 kb on + strand, within COO64_RS07700 at 32.624 kb on - strand, within COO64_RS07700 at 32.624 kb on - strand, within COO64_RS07700 at 32.836 kb on + strand at 32.837 kb on - strand at 33.034 kb on - strand at 33.034 kb on - strand at 33.334 kb on + strand, within COO64_RS07705 at 33.334 kb on + strand, within COO64_RS07705 at 33.488 kb on - strand, within COO64_RS07705 at 33.663 kb on + strand, within COO64_RS07705 at 33.663 kb on + strand, within COO64_RS07705 at 33.663 kb on + strand, within COO64_RS07705 at 33.663 kb on + strand, within COO64_RS07705 at 33.663 kb on + strand, within COO64_RS07705 at 33.663 kb on + strand, within COO64_RS07705 at 33.663 kb on + strand, within COO64_RS07705 at 33.664 kb on - strand, within COO64_RS07705 at 33.664 kb on - strand, within COO64_RS07705 at 33.664 kb on - strand, within COO64_RS07705 at 33.664 kb on - strand, within COO64_RS07705 at 33.664 kb on - strand, within COO64_RS07705 at 33.946 kb on + strand, within COO64_RS07705 at 33.946 kb on + strand, within COO64_RS07705 at 33.946 kb on + strand, within COO64_RS07705 at 33.947 kb on - strand, within COO64_RS07705 at 33.947 kb on - strand, within COO64_RS07705 at 34.154 kb on - strand, within COO64_RS07705 at 34.339 kb on + strand at 34.339 kb on + strand at 34.340 kb on - strand at 34.736 kb on + strand, within imuA
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.5X LB remove 32,008 + COO64_RS07700 0.69 -0.9 32,009 - COO64_RS07700 0.69 -1.1 32,009 - COO64_RS07700 0.69 +0.8 32,009 - COO64_RS07700 0.69 +0.1 32,017 + COO64_RS07700 0.70 -0.9 32,018 - COO64_RS07700 0.70 -1.5 32,018 - COO64_RS07700 0.70 +0.2 32,018 - COO64_RS07700 0.70 +0.8 32,623 + COO64_RS07700 0.89 +0.7 32,624 - COO64_RS07700 0.89 -1.8 32,624 - COO64_RS07700 0.89 -0.5 32,836 + +0.9 32,837 - +1.0 33,034 - +0.0 33,034 - +0.6 33,334 + COO64_RS07705 0.28 -1.6 33,334 + COO64_RS07705 0.28 +0.6 33,488 - COO64_RS07705 0.39 -1.3 33,663 + COO64_RS07705 0.51 -1.3 33,663 + COO64_RS07705 0.51 -0.4 33,663 + COO64_RS07705 0.51 -1.0 33,663 + COO64_RS07705 0.51 -0.9 33,663 + COO64_RS07705 0.51 -0.4 33,663 + COO64_RS07705 0.51 +1.3 33,663 + COO64_RS07705 0.51 -0.0 33,664 - COO64_RS07705 0.51 -0.2 33,664 - COO64_RS07705 0.51 +0.4 33,664 - COO64_RS07705 0.51 -0.7 33,664 - COO64_RS07705 0.51 +0.1 33,664 - COO64_RS07705 0.51 -0.4 33,946 + COO64_RS07705 0.71 +0.7 33,946 + COO64_RS07705 0.71 +0.0 33,946 + COO64_RS07705 0.71 +0.7 33,947 - COO64_RS07705 0.71 +0.0 33,947 - COO64_RS07705 0.71 -0.4 34,154 - COO64_RS07705 0.86 +0.1 34,339 + -0.2 34,339 + -1.3 34,340 - -0.9 34,736 + imuA COO64_RS07710 0.61 -0.3
Or see this region's nucleotide sequence