Experiment: supernatant control, M9 with 0.5X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltX and uvrB are separated by 87 nucleotides uvrB and COO64_RS05735 are separated by 308 nucleotides
COO64_RS05725: gltX - glutamate--tRNA ligase, at 661,232 to 662,713
gltX
COO64_RS05730: uvrB - excinuclease ABC subunit UvrB, at 662,801 to 664,816
uvrB
COO64_RS05735: COO64_RS05735 - amino acid aminotransferase, at 665,125 to 666,321
_RS05735
Position (kb) 662
663
664
665 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 662.754 kb on - strand at 662.834 kb on - strand at 662.889 kb on + strand at 662.889 kb on + strand at 662.889 kb on + strand at 662.889 kb on + strand at 662.889 kb on + strand at 662.889 kb on + strand at 662.890 kb on - strand at 662.890 kb on - strand at 662.890 kb on - strand at 662.890 kb on - strand at 663.165 kb on + strand, within uvrB at 663.166 kb on - strand, within uvrB at 663.429 kb on + strand, within uvrB at 663.429 kb on + strand, within uvrB at 663.429 kb on + strand, within uvrB at 663.430 kb on - strand, within uvrB at 663.430 kb on - strand, within uvrB at 663.587 kb on + strand, within uvrB at 663.588 kb on - strand, within uvrB at 663.588 kb on - strand, within uvrB at 663.588 kb on - strand, within uvrB at 663.624 kb on + strand, within uvrB at 663.624 kb on + strand, within uvrB at 663.624 kb on + strand, within uvrB at 663.624 kb on + strand, within uvrB at 663.624 kb on + strand, within uvrB at 663.624 kb on + strand, within uvrB at 663.624 kb on + strand, within uvrB at 663.624 kb on + strand, within uvrB at 663.624 kb on + strand, within uvrB at 663.625 kb on - strand, within uvrB at 663.625 kb on - strand, within uvrB at 663.625 kb on - strand, within uvrB at 663.625 kb on - strand, within uvrB at 663.625 kb on - strand, within uvrB at 663.625 kb on - strand, within uvrB at 663.625 kb on - strand, within uvrB at 663.625 kb on - strand, within uvrB at 663.730 kb on - strand, within uvrB at 663.768 kb on + strand, within uvrB at 663.768 kb on + strand, within uvrB at 663.768 kb on + strand, within uvrB at 663.768 kb on + strand, within uvrB at 663.768 kb on + strand, within uvrB at 663.768 kb on + strand, within uvrB at 663.768 kb on + strand, within uvrB at 663.768 kb on + strand, within uvrB at 663.769 kb on - strand, within uvrB at 663.769 kb on - strand, within uvrB at 663.769 kb on - strand, within uvrB at 663.769 kb on - strand, within uvrB at 663.769 kb on - strand, within uvrB at 663.769 kb on - strand, within uvrB at 663.769 kb on - strand, within uvrB at 663.837 kb on + strand, within uvrB at 663.837 kb on + strand, within uvrB at 663.838 kb on - strand, within uvrB at 663.838 kb on - strand, within uvrB at 663.838 kb on - strand, within uvrB at 663.843 kb on + strand, within uvrB at 663.844 kb on - strand, within uvrB at 663.915 kb on + strand, within uvrB at 664.002 kb on + strand, within uvrB at 664.003 kb on - strand, within uvrB at 664.003 kb on - strand, within uvrB at 664.003 kb on - strand, within uvrB at 664.003 kb on - strand, within uvrB at 664.003 kb on - strand, within uvrB at 664.033 kb on + strand, within uvrB at 664.074 kb on + strand, within uvrB at 664.074 kb on + strand, within uvrB at 664.074 kb on + strand, within uvrB at 664.074 kb on + strand, within uvrB at 664.074 kb on + strand, within uvrB at 664.074 kb on + strand, within uvrB at 664.074 kb on + strand, within uvrB at 664.075 kb on - strand, within uvrB at 664.075 kb on - strand, within uvrB at 664.075 kb on - strand, within uvrB at 664.278 kb on + strand, within uvrB at 664.278 kb on + strand, within uvrB at 664.278 kb on + strand, within uvrB at 664.279 kb on - strand, within uvrB at 664.279 kb on - strand, within uvrB at 664.359 kb on + strand, within uvrB at 664.359 kb on + strand, within uvrB at 664.360 kb on - strand, within uvrB at 664.360 kb on - strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.383 kb on + strand, within uvrB at 664.384 kb on - strand, within uvrB at 664.384 kb on - strand, within uvrB at 664.384 kb on - strand, within uvrB at 664.384 kb on - strand, within uvrB at 664.384 kb on - strand, within uvrB at 664.384 kb on - strand, within uvrB at 664.384 kb on - strand, within uvrB at 664.465 kb on + strand, within uvrB at 664.466 kb on - strand, within uvrB at 664.495 kb on + strand, within uvrB at 664.518 kb on + strand, within uvrB at 664.518 kb on + strand, within uvrB at 664.518 kb on + strand, within uvrB at 664.519 kb on - strand, within uvrB at 664.519 kb on - strand, within uvrB at 664.519 kb on - strand, within uvrB at 664.519 kb on - strand, within uvrB at 664.533 kb on + strand, within uvrB at 664.533 kb on + strand, within uvrB at 664.533 kb on + strand, within uvrB at 664.533 kb on + strand, within uvrB at 664.534 kb on - strand, within uvrB at 664.534 kb on - strand, within uvrB at 664.536 kb on + strand, within uvrB at 664.536 kb on + strand, within uvrB at 664.537 kb on - strand, within uvrB at 664.542 kb on + strand, within uvrB at 664.542 kb on + strand, within uvrB at 664.542 kb on + strand, within uvrB at 664.543 kb on - strand, within uvrB at 664.543 kb on - strand, within uvrB at 664.543 kb on - strand, within uvrB at 664.543 kb on - strand, within uvrB at 664.545 kb on + strand, within uvrB at 664.545 kb on + strand, within uvrB at 664.546 kb on - strand, within uvrB at 664.546 kb on - strand, within uvrB at 664.546 kb on - strand, within uvrB at 664.557 kb on + strand, within uvrB at 664.558 kb on - strand, within uvrB at 664.558 kb on - strand, within uvrB at 664.599 kb on + strand, within uvrB at 664.600 kb on - strand, within uvrB at 664.868 kb on - strand at 664.868 kb on - strand at 664.868 kb on - strand at 664.871 kb on + strand at 664.871 kb on + strand at 664.872 kb on - strand at 664.872 kb on - strand at 664.873 kb on + strand at 664.874 kb on - strand at 664.874 kb on - strand at 664.880 kb on + strand at 664.880 kb on + strand at 664.880 kb on + strand at 664.880 kb on + strand at 664.880 kb on + strand at 664.880 kb on + strand at 664.880 kb on + strand at 664.881 kb on - strand at 664.885 kb on - strand at 664.885 kb on - strand at 665.032 kb on - strand at 665.037 kb on + strand at 665.037 kb on + strand at 665.037 kb on + strand at 665.037 kb on + strand at 665.037 kb on + strand at 665.038 kb on - strand at 665.038 kb on - strand at 665.038 kb on - strand at 665.038 kb on - strand at 665.038 kb on - strand at 665.038 kb on - strand at 665.039 kb on + strand at 665.039 kb on + strand at 665.039 kb on + strand at 665.039 kb on + strand at 665.039 kb on + strand at 665.039 kb on + strand at 665.039 kb on + strand at 665.040 kb on - strand at 665.040 kb on - strand at 665.066 kb on + strand at 665.066 kb on + strand at 665.066 kb on + strand at 665.066 kb on + strand at 665.120 kb on + strand at 665.120 kb on + strand at 665.120 kb on + strand at 665.120 kb on + strand at 665.120 kb on + strand at 665.120 kb on + strand at 665.120 kb on + strand at 665.121 kb on - strand at 665.121 kb on - strand at 665.123 kb on + strand at 665.123 kb on + strand at 665.123 kb on + strand at 665.124 kb on - strand at 665.124 kb on - strand at 665.124 kb on - strand at 665.236 kb on + strand at 665.237 kb on - strand at 665.237 kb on - strand at 665.257 kb on - strand, within COO64_RS05735 at 665.350 kb on + strand, within COO64_RS05735 at 665.351 kb on - strand, within COO64_RS05735 at 665.351 kb on - strand, within COO64_RS05735 at 665.576 kb on - strand, within COO64_RS05735 at 665.576 kb on - strand, within COO64_RS05735 at 665.578 kb on + strand, within COO64_RS05735 at 665.578 kb on + strand, within COO64_RS05735 at 665.579 kb on - strand, within COO64_RS05735 at 665.579 kb on - strand, within COO64_RS05735 at 665.579 kb on - strand, within COO64_RS05735 at 665.729 kb on + strand, within COO64_RS05735 at 665.729 kb on + strand, within COO64_RS05735 at 665.729 kb on + strand, within COO64_RS05735 at 665.729 kb on + strand, within COO64_RS05735 at 665.729 kb on + strand, within COO64_RS05735 at 665.729 kb on + strand, within COO64_RS05735 at 665.729 kb on + strand, within COO64_RS05735 at 665.730 kb on - strand, within COO64_RS05735 at 665.730 kb on - strand, within COO64_RS05735 at 665.730 kb on - strand, within COO64_RS05735 at 665.770 kb on + strand, within COO64_RS05735 at 665.770 kb on + strand, within COO64_RS05735 at 665.770 kb on + strand, within COO64_RS05735 at 665.771 kb on - strand, within COO64_RS05735 at 665.771 kb on - strand, within COO64_RS05735 at 665.771 kb on - strand, within COO64_RS05735 at 665.813 kb on + strand, within COO64_RS05735
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.5X LB remove 662,754 - -1.6 662,834 - +0.0 662,889 + -0.6 662,889 + -0.8 662,889 + -0.8 662,889 + -0.1 662,889 + -0.2 662,889 + +0.1 662,890 - +0.7 662,890 - -2.0 662,890 - -1.9 662,890 - -0.5 663,165 + uvrB COO64_RS05730 0.18 -1.0 663,166 - uvrB COO64_RS05730 0.18 +0.5 663,429 + uvrB COO64_RS05730 0.31 +0.4 663,429 + uvrB COO64_RS05730 0.31 -1.5 663,429 + uvrB COO64_RS05730 0.31 -0.1 663,430 - uvrB COO64_RS05730 0.31 +0.2 663,430 - uvrB COO64_RS05730 0.31 -0.4 663,587 + uvrB COO64_RS05730 0.39 -0.5 663,588 - uvrB COO64_RS05730 0.39 +0.5 663,588 - uvrB COO64_RS05730 0.39 +0.6 663,588 - uvrB COO64_RS05730 0.39 -0.0 663,624 + uvrB COO64_RS05730 0.41 +0.8 663,624 + uvrB COO64_RS05730 0.41 +0.2 663,624 + uvrB COO64_RS05730 0.41 -0.5 663,624 + uvrB COO64_RS05730 0.41 -0.2 663,624 + uvrB COO64_RS05730 0.41 -1.5 663,624 + uvrB COO64_RS05730 0.41 +0.7 663,624 + uvrB COO64_RS05730 0.41 -0.5 663,624 + uvrB COO64_RS05730 0.41 +1.1 663,624 + uvrB COO64_RS05730 0.41 +0.7 663,625 - uvrB COO64_RS05730 0.41 -0.7 663,625 - uvrB COO64_RS05730 0.41 +0.2 663,625 - uvrB COO64_RS05730 0.41 +0.3 663,625 - uvrB COO64_RS05730 0.41 -1.5 663,625 - uvrB COO64_RS05730 0.41 -1.8 663,625 - uvrB COO64_RS05730 0.41 +0.1 663,625 - uvrB COO64_RS05730 0.41 -0.7 663,625 - uvrB COO64_RS05730 0.41 +0.0 663,730 - uvrB COO64_RS05730 0.46 -0.6 663,768 + uvrB COO64_RS05730 0.48 +0.4 663,768 + uvrB COO64_RS05730 0.48 +0.1 663,768 + uvrB COO64_RS05730 0.48 +0.2 663,768 + uvrB COO64_RS05730 0.48 +0.5 663,768 + uvrB COO64_RS05730 0.48 -0.0 663,768 + uvrB COO64_RS05730 0.48 -0.4 663,768 + uvrB COO64_RS05730 0.48 -0.6 663,768 + uvrB COO64_RS05730 0.48 +0.0 663,769 - uvrB COO64_RS05730 0.48 +0.1 663,769 - uvrB COO64_RS05730 0.48 +0.0 663,769 - uvrB COO64_RS05730 0.48 -1.2 663,769 - uvrB COO64_RS05730 0.48 -0.0 663,769 - uvrB COO64_RS05730 0.48 -0.7 663,769 - uvrB COO64_RS05730 0.48 +0.7 663,769 - uvrB COO64_RS05730 0.48 +0.2 663,837 + uvrB COO64_RS05730 0.51 +0.5 663,837 + uvrB COO64_RS05730 0.51 -2.4 663,838 - uvrB COO64_RS05730 0.51 -0.9 663,838 - uvrB COO64_RS05730 0.51 +0.1 663,838 - uvrB COO64_RS05730 0.51 +0.0 663,843 + uvrB COO64_RS05730 0.52 -1.5 663,844 - uvrB COO64_RS05730 0.52 +0.5 663,915 + uvrB COO64_RS05730 0.55 -0.5 664,002 + uvrB COO64_RS05730 0.60 +0.2 664,003 - uvrB COO64_RS05730 0.60 +0.2 664,003 - uvrB COO64_RS05730 0.60 -0.1 664,003 - uvrB COO64_RS05730 0.60 -0.5 664,003 - uvrB COO64_RS05730 0.60 +0.1 664,003 - uvrB COO64_RS05730 0.60 -0.2 664,033 + uvrB COO64_RS05730 0.61 -1.0 664,074 + uvrB COO64_RS05730 0.63 +0.7 664,074 + uvrB COO64_RS05730 0.63 +0.5 664,074 + uvrB COO64_RS05730 0.63 -0.6 664,074 + uvrB COO64_RS05730 0.63 -1.5 664,074 + uvrB COO64_RS05730 0.63 +0.4 664,074 + uvrB COO64_RS05730 0.63 -0.6 664,074 + uvrB COO64_RS05730 0.63 -0.2 664,075 - uvrB COO64_RS05730 0.63 -0.9 664,075 - uvrB COO64_RS05730 0.63 -0.3 664,075 - uvrB COO64_RS05730 0.63 +0.1 664,278 + uvrB COO64_RS05730 0.73 -0.4 664,278 + uvrB COO64_RS05730 0.73 -0.7 664,278 + uvrB COO64_RS05730 0.73 -0.0 664,279 - uvrB COO64_RS05730 0.73 +1.1 664,279 - uvrB COO64_RS05730 0.73 +1.5 664,359 + uvrB COO64_RS05730 0.77 -0.2 664,359 + uvrB COO64_RS05730 0.77 -1.1 664,360 - uvrB COO64_RS05730 0.77 +0.7 664,360 - uvrB COO64_RS05730 0.77 +0.0 664,383 + uvrB COO64_RS05730 0.78 +0.7 664,383 + uvrB COO64_RS05730 0.78 +0.6 664,383 + uvrB COO64_RS05730 0.78 -1.5 664,383 + uvrB COO64_RS05730 0.78 -1.2 664,383 + uvrB COO64_RS05730 0.78 +0.3 664,383 + uvrB COO64_RS05730 0.78 +1.0 664,383 + uvrB COO64_RS05730 0.78 -0.3 664,383 + uvrB COO64_RS05730 0.78 -0.2 664,383 + uvrB COO64_RS05730 0.78 -0.4 664,383 + uvrB COO64_RS05730 0.78 +0.5 664,383 + uvrB COO64_RS05730 0.78 -0.2 664,383 + uvrB COO64_RS05730 0.78 -1.1 664,384 - uvrB COO64_RS05730 0.79 +2.0 664,384 - uvrB COO64_RS05730 0.79 +0.3 664,384 - uvrB COO64_RS05730 0.79 -0.5 664,384 - uvrB COO64_RS05730 0.79 +0.4 664,384 - uvrB COO64_RS05730 0.79 +1.2 664,384 - uvrB COO64_RS05730 0.79 -0.3 664,384 - uvrB COO64_RS05730 0.79 +0.3 664,465 + uvrB COO64_RS05730 0.83 +0.5 664,466 - uvrB COO64_RS05730 0.83 +0.0 664,495 + uvrB COO64_RS05730 0.84 +0.5 664,518 + uvrB COO64_RS05730 0.85 -1.2 664,518 + uvrB COO64_RS05730 0.85 -0.4 664,518 + uvrB COO64_RS05730 0.85 +0.5 664,519 - uvrB COO64_RS05730 0.85 -2.8 664,519 - uvrB COO64_RS05730 0.85 +0.2 664,519 - uvrB COO64_RS05730 0.85 -1.1 664,519 - uvrB COO64_RS05730 0.85 -0.0 664,533 + uvrB COO64_RS05730 0.86 -0.7 664,533 + uvrB COO64_RS05730 0.86 -0.2 664,533 + uvrB COO64_RS05730 0.86 -0.9 664,533 + uvrB COO64_RS05730 0.86 +0.4 664,534 - uvrB COO64_RS05730 0.86 +1.2 664,534 - uvrB COO64_RS05730 0.86 +0.0 664,536 + uvrB COO64_RS05730 0.86 -0.7 664,536 + uvrB COO64_RS05730 0.86 +0.4 664,537 - uvrB COO64_RS05730 0.86 +0.5 664,542 + uvrB COO64_RS05730 0.86 +0.1 664,542 + uvrB COO64_RS05730 0.86 -1.3 664,542 + uvrB COO64_RS05730 0.86 -0.2 664,543 - uvrB COO64_RS05730 0.86 -2.4 664,543 - uvrB COO64_RS05730 0.86 -0.5 664,543 - uvrB COO64_RS05730 0.86 -1.1 664,543 - uvrB COO64_RS05730 0.86 -0.2 664,545 + uvrB COO64_RS05730 0.87 -0.3 664,545 + uvrB COO64_RS05730 0.87 +0.1 664,546 - uvrB COO64_RS05730 0.87 -0.1 664,546 - uvrB COO64_RS05730 0.87 -0.5 664,546 - uvrB COO64_RS05730 0.87 -0.0 664,557 + uvrB COO64_RS05730 0.87 -0.2 664,558 - uvrB COO64_RS05730 0.87 +0.9 664,558 - uvrB COO64_RS05730 0.87 -0.8 664,599 + uvrB COO64_RS05730 0.89 -0.5 664,600 - uvrB COO64_RS05730 0.89 +2.0 664,868 - -0.2 664,868 - -0.6 664,868 - +0.2 664,871 + -0.3 664,871 + +1.5 664,872 - +0.1 664,872 - +0.1 664,873 + +0.7 664,874 - -1.1 664,874 - +0.2 664,880 + -1.3 664,880 + -0.5 664,880 + +0.8 664,880 + +0.1 664,880 + -0.3 664,880 + +0.3 664,880 + +0.1 664,881 - -0.9 664,885 - -1.1 664,885 - -1.3 665,032 - -1.3 665,037 + +0.5 665,037 + +0.4 665,037 + -0.6 665,037 + +0.3 665,037 + +1.0 665,038 - -0.1 665,038 - +0.8 665,038 - +0.6 665,038 - +0.1 665,038 - -0.4 665,038 - +0.2 665,039 + -0.5 665,039 + +1.2 665,039 + +0.7 665,039 + +0.5 665,039 + -0.7 665,039 + +0.6 665,039 + +1.5 665,040 - -0.2 665,040 - -0.4 665,066 + +0.2 665,066 + +0.3 665,066 + +1.3 665,066 + +0.7 665,120 + +0.2 665,120 + +0.3 665,120 + +0.0 665,120 + +0.0 665,120 + -0.6 665,120 + +0.0 665,120 + -0.4 665,121 - +0.7 665,121 - -0.6 665,123 + +0.1 665,123 + +0.7 665,123 + -0.0 665,124 - -0.4 665,124 - -1.0 665,124 - +0.3 665,236 + -0.3 665,237 - -1.5 665,237 - +0.3 665,257 - COO64_RS05735 0.11 +0.1 665,350 + COO64_RS05735 0.19 +1.0 665,351 - COO64_RS05735 0.19 +0.7 665,351 - COO64_RS05735 0.19 -0.2 665,576 - COO64_RS05735 0.38 -0.6 665,576 - COO64_RS05735 0.38 +1.0 665,578 + COO64_RS05735 0.38 -0.9 665,578 + COO64_RS05735 0.38 +0.6 665,579 - COO64_RS05735 0.38 +0.3 665,579 - COO64_RS05735 0.38 -0.5 665,579 - COO64_RS05735 0.38 +0.4 665,729 + COO64_RS05735 0.50 -0.3 665,729 + COO64_RS05735 0.50 +0.4 665,729 + COO64_RS05735 0.50 +0.8 665,729 + COO64_RS05735 0.50 -1.1 665,729 + COO64_RS05735 0.50 -0.1 665,729 + COO64_RS05735 0.50 -0.3 665,729 + COO64_RS05735 0.50 +0.2 665,730 - COO64_RS05735 0.51 -0.5 665,730 - COO64_RS05735 0.51 +0.4 665,730 - COO64_RS05735 0.51 -1.5 665,770 + COO64_RS05735 0.54 +1.3 665,770 + COO64_RS05735 0.54 -0.9 665,770 + COO64_RS05735 0.54 +0.3 665,771 - COO64_RS05735 0.54 -0.4 665,771 - COO64_RS05735 0.54 -1.0 665,771 - COO64_RS05735 0.54 -0.2 665,813 + COO64_RS05735 0.57 +0.7
Or see this region's nucleotide sequence