Experiment: supernatant control, M9 with 0.5X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbB and rfbA overlap by 4 nucleotides rfbA and rfbC overlap by 4 nucleotides rfbC and rfbD overlap by 4 nucleotides
COO64_RS00570: rfbB - dTDP-glucose 4,6-dehydratase, at 124,322 to 125,404
rfbB
COO64_RS00575: rfbA - glucose-1-phosphate thymidylyltransferase RfbA, at 125,401 to 126,276
rfbA
COO64_RS00580: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 126,273 to 126,818
rfbC
COO64_RS00585: rfbD - dTDP-4-dehydrorhamnose reductase, at 126,815 to 127,687
rfbD
Position (kb) 125
126
127 Strain fitness (log2 ratio) -2
-1
0
1
2 at 125.301 kb on + strand at 125.301 kb on + strand at 125.301 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.354 kb on + strand at 125.355 kb on - strand at 125.355 kb on - strand at 125.451 kb on + strand at 125.477 kb on + strand at 125.632 kb on + strand, within rfbA at 125.730 kb on + strand, within rfbA at 125.798 kb on + strand, within rfbA at 125.929 kb on + strand, within rfbA at 125.929 kb on + strand, within rfbA at 125.929 kb on + strand, within rfbA at 125.929 kb on + strand, within rfbA at 126.013 kb on + strand, within rfbA at 126.013 kb on + strand, within rfbA at 126.109 kb on + strand, within rfbA at 126.244 kb on + strand at 126.244 kb on + strand at 126.244 kb on + strand at 126.244 kb on + strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.245 kb on - strand at 126.280 kb on + strand at 126.280 kb on + strand at 126.280 kb on + strand at 126.280 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.5X LB remove 125,301 + -0.9 125,301 + -0.7 125,301 + +0.1 125,354 + +0.5 125,354 + -0.5 125,354 + +0.2 125,354 + -0.5 125,354 + +0.6 125,354 + +1.7 125,354 + +0.5 125,354 + +1.3 125,355 - +0.1 125,355 - +0.8 125,451 + -0.7 125,477 + -0.5 125,632 + rfbA COO64_RS00575 0.26 +1.1 125,730 + rfbA COO64_RS00575 0.38 +0.1 125,798 + rfbA COO64_RS00575 0.45 -1.6 125,929 + rfbA COO64_RS00575 0.60 -0.1 125,929 + rfbA COO64_RS00575 0.60 +0.1 125,929 + rfbA COO64_RS00575 0.60 +0.1 125,929 + rfbA COO64_RS00575 0.60 +1.3 126,013 + rfbA COO64_RS00575 0.70 -0.7 126,013 + rfbA COO64_RS00575 0.70 -0.1 126,109 + rfbA COO64_RS00575 0.81 -0.1 126,244 + +0.1 126,244 + -0.5 126,244 + -0.6 126,244 + +0.1 126,245 - -0.7 126,245 - -0.4 126,245 - +0.9 126,245 - +0.0 126,245 - -2.2 126,245 - +0.5 126,245 - +0.4 126,280 + +0.1 126,280 + +1.9 126,280 + -1.1 126,280 + +1.3
Or see this region's nucleotide sequence