Experiment: supernatant control, M9 with 0.5X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murI and COO64_RS03250 are separated by 116 nucleotides COO64_RS03250 and COO64_RS03255 are separated by 24 nucleotides COO64_RS03255 and phrB overlap by 4 nucleotides
COO64_RS03245: murI - glutamate racemase, at 126,636 to 127,436
murI
COO64_RS03250: COO64_RS03250 - acyloxyacyl hydrolase, at 127,553 to 128,071
_RS03250
COO64_RS03255: COO64_RS03255 - SDR family NAD(P)-dependent oxidoreductase, at 128,096 to 128,818
_RS03255
COO64_RS03260: phrB - deoxyribodipyrimidine photo-lyase, at 128,815 to 130,257
phrB
Position (kb)
128
129 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 127.403 kb on + strand at 127.404 kb on - strand at 127.404 kb on - strand at 127.435 kb on - strand at 127.471 kb on + strand at 127.471 kb on + strand at 127.498 kb on - strand at 127.533 kb on + strand at 127.533 kb on + strand at 127.533 kb on + strand at 127.533 kb on + strand at 127.534 kb on - strand at 127.534 kb on - strand at 127.609 kb on + strand, within COO64_RS03250 at 127.609 kb on + strand, within COO64_RS03250 at 127.629 kb on + strand, within COO64_RS03250 at 127.629 kb on + strand, within COO64_RS03250 at 127.629 kb on + strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.630 kb on - strand, within COO64_RS03250 at 127.671 kb on - strand, within COO64_RS03250 at 127.671 kb on - strand, within COO64_RS03250 at 127.671 kb on - strand, within COO64_RS03250 at 127.717 kb on + strand, within COO64_RS03250 at 127.717 kb on + strand, within COO64_RS03250 at 127.717 kb on + strand, within COO64_RS03250 at 127.718 kb on - strand, within COO64_RS03250 at 127.718 kb on - strand, within COO64_RS03250 at 127.718 kb on - strand, within COO64_RS03250 at 127.718 kb on - strand, within COO64_RS03250 at 127.722 kb on + strand, within COO64_RS03250 at 127.722 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.754 kb on + strand, within COO64_RS03250 at 127.755 kb on - strand, within COO64_RS03250 at 127.755 kb on - strand, within COO64_RS03250 at 127.755 kb on - strand, within COO64_RS03250 at 127.755 kb on - strand, within COO64_RS03250 at 127.760 kb on + strand, within COO64_RS03250 at 127.760 kb on + strand, within COO64_RS03250 at 127.761 kb on - strand, within COO64_RS03250 at 127.761 kb on - strand, within COO64_RS03250 at 127.761 kb on - strand, within COO64_RS03250 at 127.761 kb on - strand, within COO64_RS03250 at 127.850 kb on + strand, within COO64_RS03250 at 127.850 kb on + strand, within COO64_RS03250 at 127.851 kb on - strand, within COO64_RS03250 at 127.851 kb on - strand, within COO64_RS03250 at 127.904 kb on - strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.977 kb on + strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.978 kb on - strand, within COO64_RS03250 at 127.983 kb on + strand, within COO64_RS03250 at 127.983 kb on + strand, within COO64_RS03250 at 127.983 kb on + strand, within COO64_RS03250 at 127.984 kb on - strand, within COO64_RS03250 at 127.984 kb on - strand, within COO64_RS03250 at 127.997 kb on + strand, within COO64_RS03250 at 128.054 kb on + strand at 128.054 kb on + strand at 128.055 kb on - strand at 128.059 kb on + strand at 128.059 kb on + strand at 128.060 kb on - strand at 128.060 kb on - strand at 128.060 kb on - strand at 128.060 kb on - strand at 128.060 kb on - strand at 128.097 kb on + strand at 128.097 kb on + strand at 128.098 kb on - strand at 128.098 kb on - strand at 128.296 kb on + strand, within COO64_RS03255 at 128.397 kb on + strand, within COO64_RS03255 at 128.541 kb on + strand, within COO64_RS03255 at 128.542 kb on - strand, within COO64_RS03255 at 128.654 kb on + strand, within COO64_RS03255 at 128.654 kb on + strand, within COO64_RS03255 at 128.654 kb on + strand, within COO64_RS03255 at 128.655 kb on - strand, within COO64_RS03255 at 128.655 kb on - strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.690 kb on + strand, within COO64_RS03255 at 128.691 kb on - strand, within COO64_RS03255 at 128.691 kb on - strand, within COO64_RS03255 at 128.822 kb on - strand at 128.901 kb on + strand at 128.901 kb on + strand at 128.901 kb on + strand at 128.901 kb on + strand at 128.902 kb on - strand at 128.902 kb on - strand at 128.902 kb on - strand at 128.902 kb on - strand at 128.902 kb on - strand at 128.919 kb on + strand at 128.919 kb on + strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.920 kb on - strand at 128.923 kb on - strand at 128.923 kb on - strand at 129.063 kb on - strand, within phrB at 129.404 kb on + strand, within phrB at 129.404 kb on + strand, within phrB at 129.404 kb on + strand, within phrB at 129.533 kb on + strand, within phrB at 129.533 kb on + strand, within phrB at 129.533 kb on + strand, within phrB at 129.534 kb on - strand, within phrB at 129.587 kb on + strand, within phrB at 129.587 kb on + strand, within phrB at 129.587 kb on + strand, within phrB at 129.587 kb on + strand, within phrB at 129.588 kb on - strand, within phrB at 129.664 kb on + strand, within phrB at 129.773 kb on - strand, within phrB at 129.773 kb on - strand, within phrB at 129.788 kb on + strand, within phrB at 129.788 kb on + strand, within phrB at 129.788 kb on + strand, within phrB at 129.788 kb on + strand, within phrB at 129.788 kb on + strand, within phrB
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.5X LB remove 127,403 + -0.1 127,404 - -0.5 127,404 - +0.4 127,435 - +0.6 127,471 + -0.8 127,471 + -0.9 127,498 - -0.2 127,533 + +0.3 127,533 + -0.6 127,533 + +0.4 127,533 + +0.0 127,534 - +0.4 127,534 - -0.6 127,609 + COO64_RS03250 0.11 +0.0 127,609 + COO64_RS03250 0.11 +0.1 127,629 + COO64_RS03250 0.15 -0.7 127,629 + COO64_RS03250 0.15 -1.3 127,629 + COO64_RS03250 0.15 -0.1 127,630 - COO64_RS03250 0.15 -0.3 127,630 - COO64_RS03250 0.15 -1.0 127,630 - COO64_RS03250 0.15 -0.7 127,630 - COO64_RS03250 0.15 -1.3 127,630 - COO64_RS03250 0.15 -0.9 127,671 - COO64_RS03250 0.23 +0.3 127,671 - COO64_RS03250 0.23 -0.7 127,671 - COO64_RS03250 0.23 +0.1 127,717 + COO64_RS03250 0.32 +0.4 127,717 + COO64_RS03250 0.32 +1.2 127,717 + COO64_RS03250 0.32 -1.8 127,718 - COO64_RS03250 0.32 -0.0 127,718 - COO64_RS03250 0.32 -0.0 127,718 - COO64_RS03250 0.32 -0.2 127,718 - COO64_RS03250 0.32 -0.3 127,722 + COO64_RS03250 0.33 +0.7 127,722 + COO64_RS03250 0.33 -0.6 127,754 + COO64_RS03250 0.39 -0.1 127,754 + COO64_RS03250 0.39 +0.1 127,754 + COO64_RS03250 0.39 +0.6 127,754 + COO64_RS03250 0.39 +0.8 127,754 + COO64_RS03250 0.39 -0.7 127,754 + COO64_RS03250 0.39 -1.1 127,754 + COO64_RS03250 0.39 +0.9 127,754 + COO64_RS03250 0.39 -0.6 127,755 - COO64_RS03250 0.39 -2.5 127,755 - COO64_RS03250 0.39 +1.9 127,755 - COO64_RS03250 0.39 +0.9 127,755 - COO64_RS03250 0.39 +0.2 127,760 + COO64_RS03250 0.40 -2.6 127,760 + COO64_RS03250 0.40 +0.2 127,761 - COO64_RS03250 0.40 +1.0 127,761 - COO64_RS03250 0.40 +0.1 127,761 - COO64_RS03250 0.40 -0.4 127,761 - COO64_RS03250 0.40 -0.6 127,850 + COO64_RS03250 0.57 +0.4 127,850 + COO64_RS03250 0.57 -1.2 127,851 - COO64_RS03250 0.57 +0.0 127,851 - COO64_RS03250 0.57 -0.7 127,904 - COO64_RS03250 0.68 -0.6 127,977 + COO64_RS03250 0.82 -0.0 127,977 + COO64_RS03250 0.82 +0.2 127,977 + COO64_RS03250 0.82 +0.2 127,977 + COO64_RS03250 0.82 -0.7 127,977 + COO64_RS03250 0.82 +0.1 127,978 - COO64_RS03250 0.82 -0.3 127,978 - COO64_RS03250 0.82 +0.2 127,978 - COO64_RS03250 0.82 +0.4 127,978 - COO64_RS03250 0.82 +0.8 127,978 - COO64_RS03250 0.82 +0.9 127,983 + COO64_RS03250 0.83 -1.1 127,983 + COO64_RS03250 0.83 -0.4 127,983 + COO64_RS03250 0.83 -1.0 127,984 - COO64_RS03250 0.83 -0.4 127,984 - COO64_RS03250 0.83 +0.1 127,997 + COO64_RS03250 0.86 -1.0 128,054 + +0.2 128,054 + -1.1 128,055 - -1.5 128,059 + -0.2 128,059 + -0.5 128,060 - +1.9 128,060 - -1.6 128,060 - +2.9 128,060 - -0.6 128,060 - -0.2 128,097 + +0.4 128,097 + -0.1 128,098 - -0.8 128,098 - +0.2 128,296 + COO64_RS03255 0.28 +0.7 128,397 + COO64_RS03255 0.42 -0.1 128,541 + COO64_RS03255 0.62 -0.2 128,542 - COO64_RS03255 0.62 -0.1 128,654 + COO64_RS03255 0.77 -0.0 128,654 + COO64_RS03255 0.77 +1.4 128,654 + COO64_RS03255 0.77 +0.3 128,655 - COO64_RS03255 0.77 -1.1 128,655 - COO64_RS03255 0.77 -0.4 128,690 + COO64_RS03255 0.82 -0.3 128,690 + COO64_RS03255 0.82 -0.2 128,690 + COO64_RS03255 0.82 -0.3 128,690 + COO64_RS03255 0.82 +0.2 128,690 + COO64_RS03255 0.82 +0.9 128,690 + COO64_RS03255 0.82 +0.0 128,690 + COO64_RS03255 0.82 +0.7 128,691 - COO64_RS03255 0.82 +0.3 128,691 - COO64_RS03255 0.82 +0.3 128,822 - -1.0 128,901 + -0.0 128,901 + +1.0 128,901 + +0.7 128,901 + +0.3 128,902 - +0.3 128,902 - +1.1 128,902 - +0.2 128,902 - -1.5 128,902 - -0.4 128,919 + +1.0 128,919 + +0.7 128,920 - +0.4 128,920 - +0.1 128,920 - -0.5 128,920 - -1.0 128,920 - +0.6 128,920 - +0.9 128,923 - -0.2 128,923 - -1.6 129,063 - phrB COO64_RS03260 0.17 -0.6 129,404 + phrB COO64_RS03260 0.41 +0.2 129,404 + phrB COO64_RS03260 0.41 +0.8 129,404 + phrB COO64_RS03260 0.41 -0.5 129,533 + phrB COO64_RS03260 0.50 +0.4 129,533 + phrB COO64_RS03260 0.50 -1.3 129,533 + phrB COO64_RS03260 0.50 -3.7 129,534 - phrB COO64_RS03260 0.50 -0.1 129,587 + phrB COO64_RS03260 0.53 +0.5 129,587 + phrB COO64_RS03260 0.53 -1.7 129,587 + phrB COO64_RS03260 0.53 -0.5 129,587 + phrB COO64_RS03260 0.53 -0.5 129,588 - phrB COO64_RS03260 0.54 -0.0 129,664 + phrB COO64_RS03260 0.59 -0.2 129,773 - phrB COO64_RS03260 0.66 -1.1 129,773 - phrB COO64_RS03260 0.66 +0.4 129,788 + phrB COO64_RS03260 0.67 +0.0 129,788 + phrB COO64_RS03260 0.67 +0.9 129,788 + phrB COO64_RS03260 0.67 +1.9 129,788 + phrB COO64_RS03260 0.67 +0.1 129,788 + phrB COO64_RS03260 0.67 +0.3
Or see this region's nucleotide sequence