Experiment: M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.5X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS21835 and COO64_RS21840 are separated by 6 nucleotides COO64_RS21840 and recQ are separated by 118 nucleotides recQ and COO64_RS21850 are separated by 245 nucleotides COO64_RS21850 and COO64_RS21855 are separated by 10 nucleotides
COO64_RS21835: COO64_RS21835 - patatin-like phospholipase family protein, at 132,640 to 134,829
_RS21835
COO64_RS21840: COO64_RS21840 - MarR family transcriptional regulator, at 134,836 to 135,270
_RS21840
COO64_RS21845: recQ - DNA helicase RecQ, at 135,389 to 137,527
recQ
COO64_RS21850: COO64_RS21850 - YecA family protein, at 137,773 to 138,360
_RS21850
COO64_RS21855: COO64_RS21855 - YbaN family protein, at 138,371 to 138,736
_RS21855
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 134.593 kb on + strand, within COO64_RS21835 at 134.593 kb on + strand, within COO64_RS21835 at 134.593 kb on + strand, within COO64_RS21835 at 134.593 kb on + strand, within COO64_RS21835 at 134.593 kb on + strand, within COO64_RS21835 at 134.594 kb on - strand, within COO64_RS21835 at 134.596 kb on + strand, within COO64_RS21835 at 134.597 kb on - strand, within COO64_RS21835 at 134.620 kb on + strand at 134.644 kb on + strand at 134.644 kb on + strand at 134.644 kb on + strand at 134.644 kb on + strand at 134.645 kb on - strand at 134.707 kb on + strand at 134.707 kb on + strand at 134.707 kb on + strand at 134.708 kb on - strand at 134.708 kb on - strand at 134.708 kb on - strand at 134.771 kb on - strand at 134.803 kb on + strand at 134.803 kb on + strand at 134.803 kb on + strand at 134.803 kb on + strand at 134.803 kb on + strand at 134.803 kb on + strand at 134.804 kb on - strand at 134.804 kb on - strand at 135.031 kb on + strand, within COO64_RS21840 at 135.270 kb on + strand at 135.270 kb on + strand at 135.271 kb on - strand at 135.293 kb on - strand at 135.296 kb on + strand at 135.297 kb on - strand at 135.297 kb on - strand at 135.307 kb on + strand at 135.339 kb on + strand at 135.340 kb on - strand at 135.340 kb on - strand at 135.366 kb on + strand at 135.366 kb on + strand at 135.367 kb on - strand at 135.368 kb on + strand at 135.368 kb on + strand at 135.369 kb on - strand at 135.369 kb on - strand at 135.369 kb on - strand at 135.391 kb on - strand at 135.391 kb on - strand at 135.391 kb on - strand at 135.391 kb on - strand at 135.429 kb on + strand at 135.429 kb on + strand at 135.430 kb on - strand at 135.430 kb on - strand at 135.521 kb on + strand at 135.521 kb on + strand at 135.522 kb on - strand at 135.522 kb on - strand at 135.606 kb on + strand, within recQ at 135.606 kb on + strand, within recQ at 135.606 kb on + strand, within recQ at 135.606 kb on + strand, within recQ at 135.607 kb on - strand, within recQ at 135.827 kb on + strand, within recQ at 135.827 kb on + strand, within recQ at 135.828 kb on - strand, within recQ at 135.828 kb on - strand, within recQ at 135.864 kb on + strand, within recQ at 135.864 kb on + strand, within recQ at 135.864 kb on + strand, within recQ at 135.864 kb on + strand, within recQ at 135.864 kb on + strand, within recQ at 135.864 kb on + strand, within recQ at 135.865 kb on - strand, within recQ at 135.865 kb on - strand, within recQ at 135.865 kb on - strand, within recQ at 135.865 kb on - strand, within recQ at 135.865 kb on - strand, within recQ at 135.865 kb on - strand, within recQ at 136.084 kb on - strand, within recQ at 136.084 kb on - strand, within recQ at 136.279 kb on - strand, within recQ at 136.386 kb on + strand, within recQ at 136.386 kb on + strand, within recQ at 136.386 kb on + strand, within recQ at 136.386 kb on + strand, within recQ at 136.386 kb on + strand, within recQ at 136.387 kb on - strand, within recQ at 136.387 kb on - strand, within recQ at 136.387 kb on - strand, within recQ at 136.387 kb on - strand, within recQ at 136.387 kb on - strand, within recQ at 136.533 kb on + strand, within recQ at 136.590 kb on + strand, within recQ at 136.590 kb on + strand, within recQ at 136.590 kb on + strand, within recQ at 136.590 kb on + strand, within recQ at 136.591 kb on - strand, within recQ at 136.593 kb on + strand, within recQ at 136.593 kb on + strand, within recQ at 136.594 kb on - strand, within recQ at 136.594 kb on - strand, within recQ at 136.755 kb on + strand, within recQ at 136.755 kb on + strand, within recQ at 136.756 kb on - strand, within recQ at 136.788 kb on + strand, within recQ at 136.789 kb on - strand, within recQ at 136.789 kb on - strand, within recQ at 136.789 kb on - strand, within recQ at 136.789 kb on - strand, within recQ at 136.789 kb on - strand, within recQ at 136.789 kb on - strand, within recQ at 136.789 kb on - strand, within recQ at 136.827 kb on + strand, within recQ at 136.827 kb on + strand, within recQ at 136.828 kb on - strand, within recQ at 136.828 kb on - strand, within recQ at 136.908 kb on + strand, within recQ at 136.908 kb on + strand, within recQ at 136.908 kb on + strand, within recQ at 136.909 kb on - strand, within recQ at 136.909 kb on - strand, within recQ at 136.909 kb on - strand, within recQ at 137.067 kb on + strand, within recQ at 137.068 kb on - strand, within recQ at 137.100 kb on - strand, within recQ at 137.193 kb on + strand, within recQ at 137.194 kb on - strand, within recQ at 137.221 kb on + strand, within recQ at 137.222 kb on - strand, within recQ at 137.417 kb on + strand at 137.660 kb on + strand at 137.660 kb on + strand at 137.660 kb on + strand at 137.661 kb on - strand at 137.661 kb on - strand at 137.661 kb on - strand at 137.662 kb on + strand at 137.663 kb on - strand at 137.697 kb on + strand at 137.697 kb on + strand at 137.697 kb on + strand at 137.753 kb on - strand at 137.763 kb on + strand at 137.764 kb on - strand at 137.764 kb on - strand at 137.848 kb on + strand, within COO64_RS21850 at 137.848 kb on + strand, within COO64_RS21850 at 137.849 kb on - strand, within COO64_RS21850 at 137.947 kb on + strand, within COO64_RS21850 at 137.947 kb on + strand, within COO64_RS21850 at 137.947 kb on + strand, within COO64_RS21850 at 137.947 kb on + strand, within COO64_RS21850 at 137.947 kb on + strand, within COO64_RS21850 at 137.947 kb on + strand, within COO64_RS21850 at 137.947 kb on + strand, within COO64_RS21850 at 137.948 kb on - strand, within COO64_RS21850 at 137.948 kb on - strand, within COO64_RS21850 at 137.948 kb on - strand, within COO64_RS21850 at 138.122 kb on + strand, within COO64_RS21850 at 138.122 kb on + strand, within COO64_RS21850 at 138.122 kb on + strand, within COO64_RS21850 at 138.123 kb on - strand, within COO64_RS21850 at 138.123 kb on - strand, within COO64_RS21850 at 138.301 kb on + strand, within COO64_RS21850 at 138.301 kb on + strand, within COO64_RS21850 at 138.301 kb on + strand, within COO64_RS21850 at 138.301 kb on + strand, within COO64_RS21850 at 138.302 kb on - strand at 138.302 kb on - strand at 138.302 kb on - strand at 138.302 kb on - strand at 138.377 kb on + strand at 138.378 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.5X remove 134,593 + COO64_RS21835 0.89 +0.6 134,593 + COO64_RS21835 0.89 +0.6 134,593 + COO64_RS21835 0.89 -0.5 134,593 + COO64_RS21835 0.89 +0.3 134,593 + COO64_RS21835 0.89 -1.0 134,594 - COO64_RS21835 0.89 -0.3 134,596 + COO64_RS21835 0.89 -0.2 134,597 - COO64_RS21835 0.89 +0.7 134,620 + -0.0 134,644 + +0.3 134,644 + -0.2 134,644 + +0.3 134,644 + -0.4 134,645 - -0.8 134,707 + +0.4 134,707 + -0.7 134,707 + -0.0 134,708 - -0.8 134,708 - -0.7 134,708 - -0.0 134,771 - +1.5 134,803 + +0.2 134,803 + -0.7 134,803 + +1.3 134,803 + -0.2 134,803 + +0.5 134,803 + -0.9 134,804 - +0.4 134,804 - -0.2 135,031 + COO64_RS21840 0.45 +0.4 135,270 + +0.2 135,270 + +1.0 135,271 - +1.1 135,293 - -0.9 135,296 + +0.5 135,297 - -0.2 135,297 - +0.3 135,307 + -0.1 135,339 + -0.0 135,340 - +1.0 135,340 - -0.3 135,366 + -0.9 135,366 + -1.1 135,367 - +0.4 135,368 + -0.6 135,368 + +1.3 135,369 - +0.3 135,369 - -1.1 135,369 - -1.3 135,391 - -0.2 135,391 - -0.7 135,391 - -0.2 135,391 - +0.0 135,429 + -0.8 135,429 + -0.3 135,430 - -2.5 135,430 - +1.4 135,521 + -0.7 135,521 + -0.6 135,522 - +0.6 135,522 - -2.3 135,606 + recQ COO64_RS21845 0.10 -0.0 135,606 + recQ COO64_RS21845 0.10 -2.0 135,606 + recQ COO64_RS21845 0.10 -0.6 135,606 + recQ COO64_RS21845 0.10 +0.1 135,607 - recQ COO64_RS21845 0.10 +0.7 135,827 + recQ COO64_RS21845 0.20 -0.6 135,827 + recQ COO64_RS21845 0.20 +0.3 135,828 - recQ COO64_RS21845 0.21 -1.1 135,828 - recQ COO64_RS21845 0.21 +0.1 135,864 + recQ COO64_RS21845 0.22 -0.3 135,864 + recQ COO64_RS21845 0.22 -0.6 135,864 + recQ COO64_RS21845 0.22 -0.6 135,864 + recQ COO64_RS21845 0.22 -0.3 135,864 + recQ COO64_RS21845 0.22 -0.3 135,864 + recQ COO64_RS21845 0.22 -0.0 135,865 - recQ COO64_RS21845 0.22 -0.2 135,865 - recQ COO64_RS21845 0.22 +0.3 135,865 - recQ COO64_RS21845 0.22 +0.1 135,865 - recQ COO64_RS21845 0.22 +0.3 135,865 - recQ COO64_RS21845 0.22 +0.9 135,865 - recQ COO64_RS21845 0.22 -1.2 136,084 - recQ COO64_RS21845 0.32 +0.4 136,084 - recQ COO64_RS21845 0.32 -0.8 136,279 - recQ COO64_RS21845 0.42 -0.6 136,386 + recQ COO64_RS21845 0.47 +0.1 136,386 + recQ COO64_RS21845 0.47 -0.6 136,386 + recQ COO64_RS21845 0.47 -0.9 136,386 + recQ COO64_RS21845 0.47 -1.5 136,386 + recQ COO64_RS21845 0.47 -0.6 136,387 - recQ COO64_RS21845 0.47 -2.9 136,387 - recQ COO64_RS21845 0.47 -1.9 136,387 - recQ COO64_RS21845 0.47 -0.2 136,387 - recQ COO64_RS21845 0.47 +0.6 136,387 - recQ COO64_RS21845 0.47 -0.3 136,533 + recQ COO64_RS21845 0.53 -0.2 136,590 + recQ COO64_RS21845 0.56 -0.7 136,590 + recQ COO64_RS21845 0.56 +0.6 136,590 + recQ COO64_RS21845 0.56 +1.6 136,590 + recQ COO64_RS21845 0.56 +0.4 136,591 - recQ COO64_RS21845 0.56 -1.7 136,593 + recQ COO64_RS21845 0.56 -1.2 136,593 + recQ COO64_RS21845 0.56 +0.1 136,594 - recQ COO64_RS21845 0.56 -0.2 136,594 - recQ COO64_RS21845 0.56 +0.2 136,755 + recQ COO64_RS21845 0.64 -1.1 136,755 + recQ COO64_RS21845 0.64 -1.4 136,756 - recQ COO64_RS21845 0.64 +0.2 136,788 + recQ COO64_RS21845 0.65 -2.6 136,789 - recQ COO64_RS21845 0.65 -0.3 136,789 - recQ COO64_RS21845 0.65 -1.6 136,789 - recQ COO64_RS21845 0.65 -0.3 136,789 - recQ COO64_RS21845 0.65 +1.1 136,789 - recQ COO64_RS21845 0.65 -0.3 136,789 - recQ COO64_RS21845 0.65 +0.8 136,789 - recQ COO64_RS21845 0.65 -1.0 136,827 + recQ COO64_RS21845 0.67 -1.3 136,827 + recQ COO64_RS21845 0.67 +0.0 136,828 - recQ COO64_RS21845 0.67 +0.0 136,828 - recQ COO64_RS21845 0.67 -0.2 136,908 + recQ COO64_RS21845 0.71 -0.9 136,908 + recQ COO64_RS21845 0.71 -1.2 136,908 + recQ COO64_RS21845 0.71 -1.0 136,909 - recQ COO64_RS21845 0.71 -0.2 136,909 - recQ COO64_RS21845 0.71 +1.0 136,909 - recQ COO64_RS21845 0.71 -2.4 137,067 + recQ COO64_RS21845 0.78 -0.9 137,068 - recQ COO64_RS21845 0.78 -0.9 137,100 - recQ COO64_RS21845 0.80 -0.4 137,193 + recQ COO64_RS21845 0.84 -2.3 137,194 - recQ COO64_RS21845 0.84 -0.7 137,221 + recQ COO64_RS21845 0.86 +0.3 137,222 - recQ COO64_RS21845 0.86 +0.1 137,417 + -0.3 137,660 + -0.2 137,660 + -1.4 137,660 + +1.0 137,661 - +0.1 137,661 - +0.8 137,661 - -1.3 137,662 + +0.5 137,663 - +0.7 137,697 + -0.6 137,697 + +0.3 137,697 + -1.5 137,753 - -1.3 137,763 + -0.2 137,764 - +0.8 137,764 - -0.2 137,848 + COO64_RS21850 0.13 +0.1 137,848 + COO64_RS21850 0.13 +1.8 137,849 - COO64_RS21850 0.13 -0.2 137,947 + COO64_RS21850 0.30 +2.3 137,947 + COO64_RS21850 0.30 +1.3 137,947 + COO64_RS21850 0.30 -0.7 137,947 + COO64_RS21850 0.30 -0.6 137,947 + COO64_RS21850 0.30 +1.0 137,947 + COO64_RS21850 0.30 -0.2 137,947 + COO64_RS21850 0.30 -0.7 137,948 - COO64_RS21850 0.30 -0.0 137,948 - COO64_RS21850 0.30 -0.7 137,948 - COO64_RS21850 0.30 -0.4 138,122 + COO64_RS21850 0.59 +0.8 138,122 + COO64_RS21850 0.59 -0.3 138,122 + COO64_RS21850 0.59 -1.2 138,123 - COO64_RS21850 0.60 -0.3 138,123 - COO64_RS21850 0.60 -1.2 138,301 + COO64_RS21850 0.90 +0.8 138,301 + COO64_RS21850 0.90 +0.3 138,301 + COO64_RS21850 0.90 +1.3 138,301 + COO64_RS21850 0.90 -0.3 138,302 - +0.4 138,302 - +0.7 138,302 - +0.1 138,302 - +0.4 138,377 + +0.4 138,378 - +0.1
Or see this region's nucleotide sequence