Experiment: M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.5X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS03415 and COO64_RS03420 are separated by 118 nucleotides COO64_RS03420 and COO64_RS03425 are separated by 71 nucleotides
COO64_RS03415: COO64_RS03415 - LuxR C-terminal-related transcriptional regulator, at 158,970 to 161,690
_RS03415
COO64_RS03420: COO64_RS03420 - sigma-54-dependent Fis family transcriptional regulator, at 161,809 to 163,713
_RS03420
COO64_RS03425: COO64_RS03425 - aldehyde dehydrogenase, at 163,785 to 165,278
_RS03425
Position (kb)
161
162
163
164 Strain fitness (log2 ratio)
-2
-1
0
1 at 160.861 kb on + strand, within COO64_RS03415 at 160.862 kb on - strand, within COO64_RS03415 at 161.079 kb on + strand, within COO64_RS03415 at 161.079 kb on + strand, within COO64_RS03415 at 161.080 kb on - strand, within COO64_RS03415 at 161.080 kb on - strand, within COO64_RS03415 at 161.080 kb on - strand, within COO64_RS03415 at 161.080 kb on - strand, within COO64_RS03415 at 161.295 kb on - strand, within COO64_RS03415 at 161.688 kb on + strand at 161.688 kb on + strand at 161.689 kb on - strand at 161.727 kb on + strand at 161.727 kb on + strand at 161.728 kb on - strand at 161.728 kb on - strand at 161.850 kb on + strand at 161.851 kb on - strand at 161.851 kb on - strand at 161.859 kb on - strand at 161.870 kb on + strand at 161.871 kb on - strand at 162.011 kb on + strand, within COO64_RS03420 at 162.061 kb on + strand, within COO64_RS03420 at 162.061 kb on + strand, within COO64_RS03420 at 162.061 kb on + strand, within COO64_RS03420 at 162.061 kb on + strand, within COO64_RS03420 at 162.062 kb on - strand, within COO64_RS03420 at 162.062 kb on - strand, within COO64_RS03420 at 162.299 kb on - strand, within COO64_RS03420 at 162.455 kb on - strand, within COO64_RS03420 at 162.455 kb on - strand, within COO64_RS03420 at 162.496 kb on + strand, within COO64_RS03420 at 162.546 kb on + strand, within COO64_RS03420 at 162.546 kb on + strand, within COO64_RS03420 at 162.712 kb on + strand, within COO64_RS03420 at 162.713 kb on - strand, within COO64_RS03420 at 162.713 kb on - strand, within COO64_RS03420 at 163.095 kb on + strand, within COO64_RS03420 at 163.122 kb on + strand, within COO64_RS03420 at 163.288 kb on + strand, within COO64_RS03420 at 163.288 kb on + strand, within COO64_RS03420 at 163.293 kb on + strand, within COO64_RS03420 at 163.430 kb on - strand, within COO64_RS03420 at 163.787 kb on - strand at 163.819 kb on + strand at 163.819 kb on + strand at 163.820 kb on - strand at 163.920 kb on + strand at 164.000 kb on - strand, within COO64_RS03425 at 164.085 kb on + strand, within COO64_RS03425 at 164.086 kb on - strand, within COO64_RS03425 at 164.086 kb on - strand, within COO64_RS03425 at 164.137 kb on + strand, within COO64_RS03425 at 164.138 kb on - strand, within COO64_RS03425 at 164.203 kb on + strand, within COO64_RS03425 at 164.204 kb on - strand, within COO64_RS03425 at 164.204 kb on - strand, within COO64_RS03425 at 164.204 kb on - strand, within COO64_RS03425 at 164.204 kb on - strand, within COO64_RS03425 at 164.204 kb on - strand, within COO64_RS03425 at 164.209 kb on + strand, within COO64_RS03425 at 164.210 kb on - strand, within COO64_RS03425 at 164.210 kb on - strand, within COO64_RS03425 at 164.340 kb on + strand, within COO64_RS03425 at 164.340 kb on + strand, within COO64_RS03425 at 164.340 kb on + strand, within COO64_RS03425 at 164.340 kb on + strand, within COO64_RS03425 at 164.340 kb on + strand, within COO64_RS03425 at 164.340 kb on + strand, within COO64_RS03425 at 164.341 kb on - strand, within COO64_RS03425 at 164.341 kb on - strand, within COO64_RS03425 at 164.341 kb on - strand, within COO64_RS03425 at 164.341 kb on - strand, within COO64_RS03425 at 164.344 kb on + strand, within COO64_RS03425 at 164.344 kb on + strand, within COO64_RS03425 at 164.345 kb on - strand, within COO64_RS03425 at 164.345 kb on - strand, within COO64_RS03425 at 164.345 kb on - strand, within COO64_RS03425 at 164.345 kb on - strand, within COO64_RS03425 at 164.345 kb on - strand, within COO64_RS03425 at 164.345 kb on - strand, within COO64_RS03425 at 164.644 kb on - strand, within COO64_RS03425
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.5X remove 160,861 + COO64_RS03415 0.69 -0.6 160,862 - COO64_RS03415 0.70 -0.7 161,079 + COO64_RS03415 0.78 -0.4 161,079 + COO64_RS03415 0.78 +0.4 161,080 - COO64_RS03415 0.78 +1.0 161,080 - COO64_RS03415 0.78 +0.2 161,080 - COO64_RS03415 0.78 +0.6 161,080 - COO64_RS03415 0.78 +0.4 161,295 - COO64_RS03415 0.85 -0.2 161,688 + +0.6 161,688 + -0.6 161,689 - +0.2 161,727 + +0.8 161,727 + -0.8 161,728 - -0.2 161,728 - -0.4 161,850 + -0.6 161,851 - -0.0 161,851 - +0.5 161,859 - -0.5 161,870 + +0.6 161,871 - +0.4 162,011 + COO64_RS03420 0.11 +0.6 162,061 + COO64_RS03420 0.13 +1.1 162,061 + COO64_RS03420 0.13 +0.4 162,061 + COO64_RS03420 0.13 -0.5 162,061 + COO64_RS03420 0.13 -0.2 162,062 - COO64_RS03420 0.13 -1.0 162,062 - COO64_RS03420 0.13 -0.2 162,299 - COO64_RS03420 0.26 +0.2 162,455 - COO64_RS03420 0.34 -1.1 162,455 - COO64_RS03420 0.34 +1.0 162,496 + COO64_RS03420 0.36 +0.0 162,546 + COO64_RS03420 0.39 +0.2 162,546 + COO64_RS03420 0.39 +0.9 162,712 + COO64_RS03420 0.47 -0.1 162,713 - COO64_RS03420 0.47 +1.2 162,713 - COO64_RS03420 0.47 +0.9 163,095 + COO64_RS03420 0.68 -1.3 163,122 + COO64_RS03420 0.69 -0.9 163,288 + COO64_RS03420 0.78 -0.2 163,288 + COO64_RS03420 0.78 +0.4 163,293 + COO64_RS03420 0.78 -0.6 163,430 - COO64_RS03420 0.85 -0.1 163,787 - -1.9 163,819 + +0.1 163,819 + -0.7 163,820 - -0.5 163,920 + -0.2 164,000 - COO64_RS03425 0.14 +0.8 164,085 + COO64_RS03425 0.20 -0.0 164,086 - COO64_RS03425 0.20 -0.8 164,086 - COO64_RS03425 0.20 -0.2 164,137 + COO64_RS03425 0.24 -0.5 164,138 - COO64_RS03425 0.24 +0.1 164,203 + COO64_RS03425 0.28 -0.3 164,204 - COO64_RS03425 0.28 +0.3 164,204 - COO64_RS03425 0.28 -1.1 164,204 - COO64_RS03425 0.28 -0.5 164,204 - COO64_RS03425 0.28 +0.3 164,204 - COO64_RS03425 0.28 -0.2 164,209 + COO64_RS03425 0.28 +0.1 164,210 - COO64_RS03425 0.28 -0.8 164,210 - COO64_RS03425 0.28 -0.5 164,340 + COO64_RS03425 0.37 +0.5 164,340 + COO64_RS03425 0.37 +0.3 164,340 + COO64_RS03425 0.37 -0.7 164,340 + COO64_RS03425 0.37 -0.5 164,340 + COO64_RS03425 0.37 +0.8 164,340 + COO64_RS03425 0.37 +0.4 164,341 - COO64_RS03425 0.37 +0.0 164,341 - COO64_RS03425 0.37 +0.4 164,341 - COO64_RS03425 0.37 -0.9 164,341 - COO64_RS03425 0.37 -0.7 164,344 + COO64_RS03425 0.37 -1.9 164,344 + COO64_RS03425 0.37 -0.3 164,345 - COO64_RS03425 0.37 +1.2 164,345 - COO64_RS03425 0.37 -0.6 164,345 - COO64_RS03425 0.37 -0.2 164,345 - COO64_RS03425 0.37 +0.2 164,345 - COO64_RS03425 0.37 -0.2 164,345 - COO64_RS03425 0.37 -1.0 164,644 - COO64_RS03425 0.57 -0.3
Or see this region's nucleotide sequence