Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX26_01427 and MPMX26_01428 overlap by 4 nucleotides MPMX26_01428 and MPMX26_01429 overlap by 4 nucleotides MPMX26_01429 and MPMX26_01430 are separated by 14 nucleotides MPMX26_01430 and MPMX26_01431 overlap by 8 nucleotides MPMX26_01431 and MPMX26_01432 are separated by 66 nucleotides MPMX26_01432 and MPMX26_01433 are separated by 13 nucleotides
MPMX26_01427: MPMX26_01427 - hypothetical protein, at 1,480,498 to 1,481,757
_01427
MPMX26_01428: MPMX26_01428 - hypothetical protein, at 1,481,754 to 1,482,065
_01428
MPMX26_01429: MPMX26_01429 - hypothetical protein, at 1,482,062 to 1,482,448
_01429
MPMX26_01430: MPMX26_01430 - hypothetical protein, at 1,482,463 to 1,484,787
_01430
MPMX26_01431: MPMX26_01431 - hypothetical protein, at 1,484,780 to 1,485,010
_01431
MPMX26_01432: MPMX26_01432 - hypothetical protein, at 1,485,077 to 1,485,475
_01432
MPMX26_01433: MPMX26_01433 - hypothetical protein, at 1,485,489 to 1,486,241
_01433
Position (kb) 1482
1483
1484
1485 Strain fitness (log2 ratio) -1
0
1 at 1482.356 kb on + strand, within MPMX26_01429 at 1482.750 kb on + strand, within MPMX26_01430 at 1483.075 kb on - strand, within MPMX26_01430 at 1483.910 kb on + strand, within MPMX26_01430 at 1485.253 kb on + strand, within MPMX26_01432
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 1,482,356 + MPMX26_01429 0.76 +0.2 1,482,750 + MPMX26_01430 0.12 -1.0 1,483,075 - MPMX26_01430 0.26 -1.2 1,483,910 + MPMX26_01430 0.62 +0.1 1,485,253 + MPMX26_01432 0.44 -0.1
Or see this region's nucleotide sequence