Experiment: M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.5X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gdhA and COO64_RS03015 overlap by 4 nucleotides COO64_RS03015 and COO64_RS03020 are separated by 66 nucleotides COO64_RS03020 and COO64_RS03025 are separated by 184 nucleotides
COO64_RS03010: gdhA - NADP-specific glutamate dehydrogenase, at 83,553 to 84,890
gdhA
COO64_RS03015: COO64_RS03015 - GreA/GreB family elongation factor, at 84,887 to 85,384
_RS03015
COO64_RS03020: COO64_RS03020 - hypothetical protein, at 85,451 to 85,807
_RS03020
COO64_RS03025: COO64_RS03025 - DUF4105 domain-containing protein, at 85,992 to 87,845
_RS03025
Position (kb) 84
85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 84.093 kb on + strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.147 kb on + strand, within gdhA at 84.147 kb on + strand, within gdhA at 84.148 kb on - strand, within gdhA at 84.195 kb on + strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.282 kb on + strand, within gdhA at 84.282 kb on + strand, within gdhA at 84.283 kb on - strand, within gdhA at 84.283 kb on - strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.352 kb on - strand, within gdhA at 84.617 kb on + strand, within gdhA at 84.617 kb on + strand, within gdhA at 84.792 kb on + strand at 84.793 kb on - strand at 84.793 kb on - strand at 84.819 kb on + strand at 84.820 kb on - strand at 84.820 kb on - strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.889 kb on - strand at 84.889 kb on - strand at 84.889 kb on - strand at 85.026 kb on + strand, within COO64_RS03015 at 85.026 kb on + strand, within COO64_RS03015 at 85.027 kb on - strand, within COO64_RS03015 at 85.168 kb on - strand, within COO64_RS03015 at 85.168 kb on - strand, within COO64_RS03015 at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.537 kb on - strand, within COO64_RS03020 at 85.605 kb on + strand, within COO64_RS03020 at 85.606 kb on - strand, within COO64_RS03020 at 85.606 kb on - strand, within COO64_RS03020 at 85.624 kb on - strand, within COO64_RS03020 at 85.624 kb on - strand, within COO64_RS03020 at 85.778 kb on + strand at 85.779 kb on - strand at 85.838 kb on + strand at 85.839 kb on - strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.940 kb on + strand at 85.941 kb on - strand at 85.941 kb on - strand at 85.941 kb on - strand at 85.941 kb on - strand at 85.993 kb on + strand at 85.993 kb on + strand at 85.993 kb on + strand at 85.993 kb on + strand at 85.994 kb on - strand at 85.994 kb on - strand at 85.994 kb on - strand at 85.994 kb on - strand at 85.994 kb on - strand at 85.996 kb on + strand at 85.996 kb on + strand at 85.997 kb on - strand at 85.997 kb on - strand at 85.997 kb on - strand at 85.997 kb on - strand at 86.002 kb on + strand at 86.149 kb on + strand at 86.150 kb on - strand at 86.150 kb on - strand at 86.312 kb on + strand, within COO64_RS03025 at 86.346 kb on + strand, within COO64_RS03025 at 86.347 kb on - strand, within COO64_RS03025 at 86.347 kb on - strand, within COO64_RS03025 at 86.347 kb on - strand, within COO64_RS03025
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.5X remove 84,093 + gdhA COO64_RS03010 0.40 +0.2 84,093 + gdhA COO64_RS03010 0.40 -0.3 84,093 + gdhA COO64_RS03010 0.40 +0.4 84,094 - gdhA COO64_RS03010 0.40 -1.0 84,094 - gdhA COO64_RS03010 0.40 +0.2 84,094 - gdhA COO64_RS03010 0.40 -0.1 84,147 + gdhA COO64_RS03010 0.44 +0.7 84,147 + gdhA COO64_RS03010 0.44 +0.2 84,148 - gdhA COO64_RS03010 0.44 -0.1 84,195 + gdhA COO64_RS03010 0.48 -1.0 84,196 - gdhA COO64_RS03010 0.48 +1.1 84,196 - gdhA COO64_RS03010 0.48 -1.0 84,196 - gdhA COO64_RS03010 0.48 +0.0 84,282 + gdhA COO64_RS03010 0.54 -0.6 84,282 + gdhA COO64_RS03010 0.54 +0.6 84,283 - gdhA COO64_RS03010 0.55 -0.4 84,283 - gdhA COO64_RS03010 0.55 +0.4 84,351 + gdhA COO64_RS03010 0.60 +1.3 84,351 + gdhA COO64_RS03010 0.60 -0.8 84,351 + gdhA COO64_RS03010 0.60 +0.9 84,351 + gdhA COO64_RS03010 0.60 +0.7 84,352 - gdhA COO64_RS03010 0.60 +0.2 84,617 + gdhA COO64_RS03010 0.80 +0.8 84,617 + gdhA COO64_RS03010 0.80 +0.2 84,792 + -1.2 84,793 - -2.3 84,793 - -0.1 84,819 + -0.1 84,820 - -0.0 84,820 - -0.8 84,888 + +0.6 84,888 + +0.0 84,888 + -0.4 84,888 + +0.8 84,889 - -0.5 84,889 - +0.2 84,889 - -0.7 85,026 + COO64_RS03015 0.28 +0.4 85,026 + COO64_RS03015 0.28 -0.2 85,027 - COO64_RS03015 0.28 +0.5 85,168 - COO64_RS03015 0.56 -0.2 85,168 - COO64_RS03015 0.56 +1.3 85,452 + +1.0 85,452 + +0.8 85,452 + -0.6 85,452 + +0.1 85,452 + -0.8 85,452 + -0.1 85,452 + -0.3 85,453 - -0.7 85,453 - +0.0 85,453 - +0.6 85,453 - +0.6 85,537 - COO64_RS03020 0.24 +0.1 85,605 + COO64_RS03020 0.43 -0.0 85,606 - COO64_RS03020 0.43 -0.3 85,606 - COO64_RS03020 0.43 -0.7 85,624 - COO64_RS03020 0.48 +0.8 85,624 - COO64_RS03020 0.48 +1.2 85,778 + -0.2 85,779 - -0.2 85,838 + -0.4 85,839 - +0.1 85,940 + -2.8 85,940 + +0.8 85,940 + -0.2 85,940 + -0.4 85,940 + +0.0 85,940 + +0.2 85,940 + -0.8 85,940 + +0.2 85,941 - -1.0 85,941 - -0.4 85,941 - +1.5 85,941 - +0.7 85,993 + -0.4 85,993 + -0.3 85,993 + -0.3 85,993 + +0.0 85,994 - -0.7 85,994 - +1.1 85,994 - +0.2 85,994 - -0.7 85,994 - +0.8 85,996 + +0.4 85,996 + +0.4 85,997 - -0.8 85,997 - -0.6 85,997 - -0.2 85,997 - +0.5 86,002 + +0.1 86,149 + -0.0 86,150 - -0.8 86,150 - +0.2 86,312 + COO64_RS03025 0.17 +0.3 86,346 + COO64_RS03025 0.19 -0.6 86,347 - COO64_RS03025 0.19 -0.0 86,347 - COO64_RS03025 0.19 +0.1 86,347 - COO64_RS03025 0.19 -0.7
Or see this region's nucleotide sequence