Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS10370 and COO64_RS10375 are separated by 211 nucleotides COO64_RS10375 and COO64_RS10380 are separated by 160 nucleotides COO64_RS10380 and COO64_RS26900 are separated by 45 nucleotides COO64_RS26900 and thrS are separated by 143 nucleotides
COO64_RS10370: COO64_RS10370 - I78 family peptidase inhibitor, at 224,429 to 224,749
_RS10370
COO64_RS10375: COO64_RS10375 - cold-shock protein, at 224,961 to 225,173
_RS10375
COO64_RS10380: COO64_RS10380 - hypothetical protein, at 225,334 to 225,630
_RS10380
COO64_RS26900: COO64_RS26900 - hypothetical protein, at 225,676 to 225,870
_RS26900
COO64_RS10385: thrS - threonine--tRNA ligase, at 226,014 to 227,936
thrS
Position (kb) 225
226 Strain fitness (log2 ratio) -2
-1
0
1 at 224.840 kb on + strand at 224.840 kb on + strand at 224.908 kb on + strand at 224.962 kb on + strand at 224.962 kb on + strand at 224.962 kb on + strand at 224.962 kb on + strand at 224.963 kb on - strand at 224.963 kb on - strand at 225.147 kb on + strand, within COO64_RS10375 at 225.148 kb on - strand, within COO64_RS10375 at 225.179 kb on + strand at 225.179 kb on + strand at 225.179 kb on + strand at 225.180 kb on - strand at 225.204 kb on + strand at 225.204 kb on + strand at 225.205 kb on - strand at 225.248 kb on + strand at 225.248 kb on + strand at 225.249 kb on - strand at 225.253 kb on + strand at 225.258 kb on - strand at 225.267 kb on + strand at 225.277 kb on + strand at 225.278 kb on - strand at 225.278 kb on - strand at 225.278 kb on - strand at 225.318 kb on + strand at 225.319 kb on - strand at 225.319 kb on - strand at 225.527 kb on - strand, within COO64_RS10380 at 225.543 kb on - strand, within COO64_RS10380 at 225.543 kb on - strand, within COO64_RS10380 at 225.665 kb on + strand at 225.665 kb on + strand at 225.665 kb on + strand at 225.665 kb on + strand at 225.666 kb on - strand at 225.722 kb on + strand, within COO64_RS26900 at 225.784 kb on + strand, within COO64_RS26900 at 225.784 kb on + strand, within COO64_RS26900 at 225.859 kb on + strand at 225.859 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 224,840 + +0.4 224,840 + +0.9 224,908 + -0.0 224,962 + -1.7 224,962 + +0.1 224,962 + -0.8 224,962 + -1.0 224,963 - +0.1 224,963 - -1.3 225,147 + COO64_RS10375 0.87 +0.5 225,148 - COO64_RS10375 0.88 -0.5 225,179 + +0.5 225,179 + +0.0 225,179 + +0.5 225,180 - -1.1 225,204 + +0.1 225,204 + -0.2 225,205 - -0.6 225,248 + -0.2 225,248 + +0.9 225,249 - -0.3 225,253 + -0.2 225,258 - +0.0 225,267 + +0.2 225,277 + -1.3 225,278 - -1.3 225,278 - -0.2 225,278 - -0.2 225,318 + +0.5 225,319 - -0.7 225,319 - +0.7 225,527 - COO64_RS10380 0.65 +1.2 225,543 - COO64_RS10380 0.70 +0.9 225,543 - COO64_RS10380 0.70 -0.8 225,665 + +0.4 225,665 + +0.2 225,665 + +0.1 225,665 + -0.6 225,666 - +0.6 225,722 + COO64_RS26900 0.24 -2.6 225,784 + COO64_RS26900 0.55 -0.6 225,784 + COO64_RS26900 0.55 +0.8 225,859 + -0.9 225,859 + -1.2
Or see this region's nucleotide sequence